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Volumn 70, Issue 16, 2005, Pages 6274-6281

Preparation of de novo globular proteins based on proline dendrimers

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CONFORMATIONS; DENDRIMERS; PLASTICITY; SYNTHESIS (CHEMICAL);

EID: 23044440151     PISSN: 00223263     EISSN: None     Source Type: Journal    
DOI: 10.1021/jo050720u     Document Type: Article
Times cited : (24)

References (41)
  • 1
    • 33544455890 scopus 로고    scopus 로고
    • note
    • 1Abbreviations: AB, 3-(4-hydroxymethylphenoxy)propionic acid; AM, p{(R,S)-amino-2,4-dimethoxybenzyl}-phenoxyacetic acid; Boc, t-butoxycarbonyl; CD, circular dichroism; DBU, 1,8-diazabicyclo[5.4.0]-undec-7- ene; DCM, dichloromethane; DIEA, N,N′-diisopropylethylamine; DIPCDI, N,N′-diisopropylcarbodiimide; DMAP, 4-dimethylaminopiridine; DMF, N,N-dimethylformamide; EtOH, ethanol; ESI-MS, electrospray ionization mass spectrometry; Fmoc, 9-fluorenylmethoxycarbonyl; G2-4, second, third and fourth generation; AcOH, acetic acid; HOBt, 1-hydroxybenzotriazole; Imd, imidazolidine-2-carboxylic acid; MBHA resin, 4-methylbenzhydrylamine resin; MALDI-TOF, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry; MeOH, methanol; NMP, 1-methyl-2-pyrrolidone; PyAOP, 7-aza-benzotriazol-1-yl-N-oxy-tris(pyrrolidino)-phosphonium hexafluorophosphate; RP-HPLC, reverse-phase high performance liquid chromatography; SEC, size exclusion chromatography; SPPS, solid-phase peptide synthesis; TFA, trifluoroacetic acid.
  • 25
    • 33544461652 scopus 로고    scopus 로고
    • note
    • Although MS is considered a qualitative analytical method, in this case it is useful to determine small amount of deletion peptides, which are formed from the same residue, and therefore should have a response very similar to MS. Other techniques such as HPLC are not useful due to structural similarity of impurities.
  • 28
    • 0032567305 scopus 로고    scopus 로고
    • For these kind couplings where excess of coupling reagent is present, phosphonium salts such as PyAOP are preferred to aminum/ uronium salts, because the latter can give guanidinium formation. Albericio, F.; Bofill, J. M.; El-Faham, A.; Kates, S. A. J. Org. Chem. 1998, 26, 9678-9683.
    • (1998) J. Org. Chem , vol.26 , pp. 9678-9683
    • Albericio, F.1    Bofill, J.M.2    El-Faham, A.3    Kates, S.A.4
  • 31
    • 33544474286 scopus 로고    scopus 로고
    • note
    • These signals came from the low percentage of Pro deletion in building block 1, and its intensity was amplified due the dendritic architecture itself.
  • 32
    • 33544468300 scopus 로고    scopus 로고
    • note
    • Several buffers were studied as mobile phases.
  • 35
    • 33544463529 scopus 로고    scopus 로고
    • note
    • This was taken as a reference to show that the presence of an additional residue of Gly does not disrupt the expected polyproline secondary structure.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.