Isolation, purification, and characterization of bovine heart and yeast cytochromes c: An integrated biochemistry laboratory experience

Biochemistry and Molecular Biology Education

Volumn 31, Issue 4, 2003, Pages 242-248

  Hunnes, Cristi H ^a  

^a Rocky Mountain College   (United States)

Author keywords

Chromatography; Cytochrome c; Electrochemistry; Electrophoresis; Laboratory instruction; Protein purification

Indexed keywords

AMMONIUM SULFATE; CYTOCHROME C; PROTEIN;

ARTICLE; CHROMATOGRAPHY; ELECTROCHEMISTRY; ELECTROPHORESIS; GEL FILTRATION CHROMATOGRAPHY; HEART; LABORATORY; MOLECULAR WEIGHT; NONHUMAN; PROTEIN PURIFICATION; SPECTROSCOPY; ULTRAFILTRATION; ULTRAVIOLET RADIATION; YEAST;

BOS TAURUS;

EID: 22944455002     PISSN: 14708175     EISSN: None     Source Type: Journal    
DOI: 10.1002/bmb.2003.494031040248     Document Type: Article

References (24)

1. J. G. Harman, J. A. Anderson, R. A. Nakashima, R. W. Shaw (1995) An integrated approach to the undergraduate biochemistry laboratory, J. Chem. Educ. 72, 641-645.
2. A. J. Wolfson, M. L. Hall, T. R. Branham (1996) An integrated biochemistry laboratory, including molecular modeling, J. Chem. Educ. 73, 1026-1028.
3. B. R. Singh (1999) A single protein research integrated advanced biochemistry laboratory course: design and general outline, Biochem. Educ. 27, 41-44.
4. P. A. Craig (1999) A project-oriented biochemistry laboratory course, J. Chem. Educ. 76, 1130-1135.
5. R. R. Peterson, J. R. Cox (2001) Integrating computational chemistry into a project-oriented biochemistry laboratory experience: a new twist on the lysozyme experiment, J. Chem. Educ. 78, 1551-1555.
6. J. A. Smiley (2002) The most proficient enzyme as the central theme in an integrated, research-based biochemistry laboratory course, Biochem. Mol. Biol. Educ. 30, 45-50.
7. Z. Abdullaev, M. E. Bodrova, B. V. Chernyak, D. A. Dolgikh, R. M. Kluck, M. O. Pereverzev, A. S. Arseniev, R. G. Efremov, M. P. Kirpichnikov, E. N. Mokhova, D. D. Newmeyer, H. Roder, V. P. Skulachev (2002) A cytochrome c mutant with high electron transfer and antioxidant activities but devoid of apoptogenic effect, Biochem. J. 362, 749-754.
8. S. Akiyama, S. Takahashi, T. Kimura, K. Ishimori, I. Morishima, Y. Nishikawa, T. Fujisawa (2002) Conformational landscape of cytochrome c folding studied by microsecond-resolved small-angle x-ray scattering, Proc. Natl. Acad. Sci. U. S. A. 99, 1329-1334.
9. J. K. Chin, R. Jimenez, F. E. Romesberg (2002) Protein dynamics and cytochrome c: correlations between ligand vibrations and redox activity, J. Am. Chem. Soc. 124, 1846-1847.
10. J. G. Pastorino, N. Shulga, J. B. Hoek (2002) Mitochondrial binding of hexokinase II inhibits Bax-induced cytochrome c release and apoptosis, J. Biol. Chem. 277, 7610-7618.
11. P. Hildebrandt, D. H. Murgida (2002) Electron transfer dynamics of cytochrome c bound to self-assembled monolayers on silver electrodes, Bioelectrochemistry 55, 139-143.
12. E. Margoliash, O. F. Walasek (1967) Cytochrome c from vertebrate and invertebrate sources, Methods Enzymol. 10, 339-348.
13. D. L. Brautigan, S. Ferguson-Miller, E. Margoliash (1978) Mitochondrial cytochrome c: preparation and activity of native and chemically modified cytochromes c, Methods Enzymol. 53, 128-164.
14. D. O. Lambeth (1979) The purification of cytochrome c from bakers' yeast, J. Chem. Educ. 56, 270-272.
15. M. P. Deutscher, Ed. (1990) Methods in Enzymology, Vol. 182, Academic Press, New York.
16. P. A. Mabrouk (1996) The purification of horse heart cytochrome c by ion exchange chromatography, J. Chem. Educ. 73, A149-A152.
17. E. L. V. Harris, S. Angal (1995) Protein Purification Methods: A Practical Approach, Oxford University Press, New York.
18. M. J. Eddowes, H. A. O. Hill (1977) Novel method for the investigation of the electrochemistry of metalloproteins: cytochrome c, J. Chem. Soc., Chem. Commun. 771-772.
19. P. M. Allen, H. A. O. Hill, N. J. Walton (1984) Surface modifiers for the promotion of direct electrochemistry of cytochrome c, J. Electroanal. Chem. 178, 69-86.
20. R. A. Scott, A. G. Mauk, Eds. (1996) Cytochrome c: A Multidisciplinary Approach, University Science Books, Sausalito, CA.
21. D. H. Evans, K. M. O'Connell, R. A. Petersen, M. J. Kelly (1983) Cyclic voltammetry, J. Chem. Educ. 60, 290-293.
22. J. Osteryoung (1983) Pulse voltammetry, J. Chem. Educ. 60, 296-298.
23. P. T. Kissinger, W. R. Heineman (1996) Laboratory Techniques in Electroanalytical Chemistry, Marcel Dekker, Inc., New York.
24. P. H. Rieger (1994) Electrochemistry, Chapman & Hall, New York.