Field-emission scanning electron microscopy analysis of morphology and enzyme distribution within an industrial biocatalytic particle

Scanning

Volumn 27, Issue 4, 2005, Pages 181-189

  Van Roon, J L ^a   Van Aelst, A C ^a   Schroen C G P H ^a   Tramper, J ^a   Beeftink, H H ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Biocatalytic particle; Distribution; Enzyme; Morphology; Scanning electron microscopy

Indexed keywords

CHEMICAL ANALYSIS; ENZYME IMMOBILIZATION; ENZYMES; INFORMATION ANALYSIS; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY;

BIOCATALYSIS; BIOCATALYTIC PARTICLES; MICROSCOPIC TECHNIQUES;

MORPHOLOGY;

ASSEMBLASE; EPITOPE; PENICILLIN AMIDASE; PENICILLIN G ACYLASE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BIOCATALYST; EMBEDDING; ENZYME LOCALIZATION; ENZYME STRUCTURE; FIELD EMISSION SCANNING ELECTRON MICROSCOPY; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; TECHNIQUE;

ANATOMY; SCANNING ELECTRON MICROSCOPY;

EID: 22944432898     PISSN: 01610457     EISSN: None     Source Type: Journal    
DOI: 10.1002/sca.4950270405     Document Type: Article

References (14)

1. Baskin TI, Busby CH, Fowke LC, Sammut M, Gubler F: Improvements in immunostaining samples embedded in methacrylate: Localization of microtubules and other antigens throughout developing organs in plants of diverse taxa. Planta 405-413 (1992)
2. De Vroom E: An improved immobilized penicillin G acylase. International Patent Application WO 97/04086 (1997)
3. Harder A, De Haan BR, Van Der Plaat JB: Novel immobilized biocatalysts and their preparation and use. International Patent Application Gist-brocades EP0222462B1 (1987)
4. Hossain MdM, Do DD: General theory of determining intraparticle active immobilized enzyme distribution and rate parameters. Biotech Bioeng 33, 963-975 (1989)
5. Kaasgaard SG, Karlsen LG, Schneider I: Process for separation of two solid components. International Patent Application WO 92/12782 (1992)
6. Scharer A, Hossain MdM, Do DD: Determination of total and active immobilized enzyme distribution in porous solid supports. Biotech Bioeng 39, 679-687 (1992)
7. Schroën CGPH, Fretz CB, De Bruin VH, Berendsen W, Moody HM, Roos EC, Van Roon JL, Kroon PJ, Strubel M, Janssen AE, Tramper J: Modeling of the enzymatic kinetically controlled synthesis of cephalexin: influence of diffusion limitation. Biotech Bioeng 80, 331-340 (2002)
8. Schroën CGPH, Nierstrasz VA, Moody HM, Hoogschagen MJ, Kroon PJ, Bosma R, Beeftink HH, Janssen AEM, Tramper J: Modeling of the enzymatic kinetic synthesis of cephalexin Influence of substrate concentration and temperature. Biotech Bioeng 73, 171-178 (2001)
9. Straathof AJJ, Panke S, Schmid A: The production of fine chemicals by biotransformations. Curr Opin Biotech 13, 548-556 (2002)
10. Tramper J, Beeftink HH, Jansen AEM, Ooijkaas LP, Van Roon JL, Strubel M, Schroën CGPH: Biocatalytic production of semi-synthetic cephalosporins: Process technology and integration. In Synthesis of Lactam Antibiotics: Chemistry, Biocatalysis & Process Integration (Ed. Bruggink A). Kluwer Academic Publishers, Dordrecht (2001)
11. Van Roon JL, Beeftink HH, Schroën CGPH, Tramper J: Assessment of intraparticle biocatalytic distributions as a tool in rational formulation. Curr Opin Biotech 13, 398-405 (2002)
12. Van Roon JL, Boom RM, Paasman M, Tramper J, Schroën CGPH, Beeftink HH: Enzyme distribution and matrix characteristics in biocatalytic particles. J Biotechnol (Accepted for publication)
13. Van Roon JL, Groenendijk E, Kieft H, Schroën CGPH, Tramper J, Beeftink HH: Novel approach to quantify immobilized-enzyme distributions. Biotechnol Bioeng 89, 660-669 (2005)
14. Yin YJ, Yao KD, Cheng GX, Ma JB: Properties of polyclectrolyte complex films of chitosan and gelatin. Polymer Internat 48, 429-432 (1999)