Functional characterization of two human olfactory receptors expressed in the baculovirus Sf9 insect cell system

Chemical Senses

Volumn 30, Issue 3, 2005, Pages 195-207

  Matarazzo, Valéry ^a   Clot Faybesse, Olivier ^a   Marcet, Brice ^a   Guiraudie Capraz, Gaëlle ^a   Atanasova, Boriana ^b   Devauchelle, Gérard ^c   Cerutti, Martine ^c   Etiévant, Patrick ^b   Ronin, Catherine ^a  

^a AIX MARSEILLE UNIVERSITÉ   (France)

^b CEMAGREF   (France)

^c CNRS   (France)

Author keywords

Baculovirus; Calcium imaging; Confocal microscopy; GPCR; Insect cell system; Olfactory receptor

Indexed keywords

CALCIUM; ESTER; KETONE; RECEPTOR PROTEIN; VIRUS VECTOR; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; OLFACTORY G PROTEIN SUBUNIT ALPHA OLF;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CALCIUM SIGNALING; CONFOCAL MICROSCOPY; HETEROLOGOUS EXPRESSION; HUMAN; INSECT CELL; LIGAND BINDING; NONHUMAN; OLFACTORY DISCRIMINATION; OLFACTORY RECEPTOR; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SMELLING; ANIMAL; BACULOVIRUS; CELL LINE; COMPARATIVE STUDY; CYTOLOGY; GENETICS; GENOMICS; INSECT; METABOLISM; ODOR;

INSECTA; MAMMALIA;

ANIMALS; BACULOVIRIDAE; CALCIUM; CELL LINE; GENOMICS; GTP-BINDING PROTEIN ALPHA SUBUNITS; HUMANS; INSECTS; MICROSCOPY, CONFOCAL; ODORS; OLFACTORY RECEPTOR NEURONS; RECEPTORS, G-PROTEIN-COUPLED;

EID: 22844434585     PISSN: 0379864X     EISSN: None     Source Type: Journal    
DOI: 10.1093/chemse/bji015     Document Type: Article

References (42)

1. Barr, A.J., Brass, L.F. and Manning, D.R. (1997) Reconstitution of receptors and GTP-binding regulatory proteins (G proteins) in Sf9 cells. A direct evaluation of selectivity in receptorG protein coupling. J. Biol. Chem., 272, 2223-2229.
2. Beets, J. (1971) Olfactory response and molecular structure. Handbook Sens. Physiol., 4, 257-321.
3. Ben-Arie, N., Lancet, D., Taylor, C., Khen, M., Walker, N., Ledbetter, D.H., Carrozzo, R., Patel, K., Sheer, D. and Lehrach, H. (1994) Olfactory receptor gene cluster on human chromosome 17: possible duplication of an ancestral receptor repertoire. Hum. Mol. Genet., 3, 229-235.
4. Blanc, S., Cerutti, M., Usmany, M., Vlak, J.M. and Hull, R. (1993) Biological activity of cauliflower mosaic virus aphid transmission factor expressed in a heterologous system. Virology, 192, 643-650.
5. Breer, H. (1994) Odor recognition and second messenger signaling in olfactory receptor neurons. Semin. Cell Biol., 5, 25-32.
6. Butkerait, P., Zheng, Y., Hallak, H., Graham, T.E., Miller, H.A., Burris, K.D., Molinoff, P.B. and Manning, D.R. (1995) Expression of the human 5-hydroxytryptamine1A receptor in Sf9 cells. Reconstitution of a coupled phenotype by co-expression of mammalian G protein subunits. J. Biol. Chem., 270, 18691-18699.
7. Chastrette, M., Thomas-Danguin, T. and Rallet, E. (1998) Modelling the human olfactory stimulus-response function. Chem. Senses, 23, 181-196.
8. Crowe, M.L., Perry, B.N. and Connerton, I.F. (1996) Olfactory receptorencoding genes and pseudogenes are expressed in humans. Gene, 169, 247-249.
9. Duchamp-Viret, P., Chaput, M.A. and Duchamp, A. (1999) Odor response properties of rat olfactory receptor neurons. Science, 284, 2171-2174.
10. Gaillard, I., Rouquier, S., Pin, J.-P., Mollard, P., Richard, S., Barnabé, C., Demaille, J. and Giorgi, D. (2002) A single olfactory receptor specifically binds a set of odorant molecules. Eur. J. Neurosci., 15, 409-418.
11. Gaymard, F., Cérutti M., Horeau, C., Lemaillet, G., Urbach, S., Ravallec, M., Devauchelle, G., Sentenac, H. and Thibaud, J.B. (1996) The channel from Arabidopsis thaliana baculovirus/insect cell system as an alternative to Xenopus oocytes. First characterization of the AKT1 K+. J. Biol. Chem., 271, 22863-22870.
12. Gilad, Y., Man, O., Paabo, S. and Lancet, D. (2003) Human specific loss of olfactory receptor genes. Proc. Natl Acad. Sci. USA, 100, 3324-3327.
13. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem., 25, 3440-3450.
14. Hatt, H., Gisselmann, G. and Wetzel, C. (1999) Cloning, functional expression and characterization of a human olfactory receptor. Cell. Mol. Biol., 45, 285-291.
15. 2+ signaling in Sf9 insect cells and the functional expression of a rat brain M5 muscarinic receptor. Am. J. Physiol., 266, 1736-1743.
16. Ivic, L., Zhang, C., Zhang, X., Yoon, S.O. and Firenstein, S.. (2002) Intracellular trafficking of a tagged and functional mammalian olfactory receptor. J. Neurobiol., 50, 56-58.
17. olf: An olfactory neuron specific-G protein involved in odorant signal transduction. Science, 244, 790-795.
18. Kajiya, K., Inaki, K., Tanaka, M., Haga, T., Kataoka, H. and Touhara, K. (2001) Molecular bases of odor discrimination: reconstitution of olfactory receptors that recognize overlapping set of odorants. J. Neurosci., 16, 6018-6025.
19. Katada, S., Nakagawa, T., Katoaka, H. and Touhara, K. (2003) Odorant response assays for a heterologously expressed olfactory receptor. Biochem. Biophys. Res. Commun., 305, 964-969.
20. Krautwurst D., Yau, K.W. and Reed R. (1998) Identification of ligands for olfactory receptors by functional expression of a receptor library. Cell, 95, 917-926.
21. Leopoldt, D., Harteneck, C. and Nurnberg, B. (1997) G-proteins endogenously expressed in Sf9 cells:interactions with mammalian histamine receptors. Naunyn Schmiedebergs Arch. Pharmacol., 356, 216-224.
22. Levasseur, G., Persuy, M.A., Grebert, D., Remy, J.J., Salesse, R. and Pajot-Augy, E. (2003) Ligand-specific dose-response of heterologously expressed olfactory receptors. Eur. J. Biochem., 270, 2905-2912.
23. Lu, M., Echeverri, F. and Moyer, B.D. (2003) Endoplasmic reticulum retention, degradation and aggregation of olfactory G-protein coupled receptors. Traffic, 6, 416-433.
24. Malnic, B., Hirono, J., Sato, T. and Buck, L.B. (1999) Combinatorial receptor codes for odors. Cell 96, 713-723.
25. Malnic, B., Godfrey, P.A. and Buck, L.B. (2004) The human olfactory receptor gene family. Proc. Natl Acad. Sci. USA, 101, 2584-2589.
26. Marcet, B., Chappe, V., Delmas, P. Gola, M. and Verrier, B. (2003) Negative regulation of CFTR activity by extracellular ATP involves P2Y2 receptors in CFTR-expressing CHO cells. J. Membr. Biol., 194, 21-32.
27. Marchal, I., Cérutti, M., Mir, A.M., Juliant, S., Devauchelle, G., Cacan, R. and Verbert, A. (2001) Expression of a membrane-bound form of Trypanosoma cruzei trans-sialidase in baculovirus-infected cells: A potential tool for sialylation of glycoproteins produced in the baculovirus-insect cells system. Glycobiology, 11, 593-603.
28. Matarazzo, V., Zsürger, N., Guillemot, J.C., Clot-Faybesse, O., Botto, J.M., Dal Farra, D., Crowe, M., Demaille, J., Vincent, P., Mazella, J. and Ronin, C. (2002) Porcine odorant-binding protein selectively binds to a human olfactory receptor. Chem. Senses, 27, 691-701.
29. McClintock, T.S., Landers, T.M., Gimelbrant, A.A., Fuller, L.Z., Jackson, B.A., Jayawickreme, C.K. and Lerner, M.R. (1997) Functional expression of olfactory-adrenergic receptor chimeras and intracellular retention of heterolously expressed olfactory receptors. Brain Res. Mol. Brain Res., 48, 270-278.
30. Menashe, I., Man, O., Lancet, D. and Gilad, Y. (2003) Different noses different people. Nat. Genet., 34, 143-144.
31. Mombaerts, P. (2001) How smell develops. Nat. Neurosci. Suppl., 4, 1192-1198.
32. Parker, E.M., Kameyama, K., Higashijima, T. and Ross, E.M. (1991) Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells. J. Biol. Chem., 266, 519-527.
33. Poul, M.A., Cerutti, M., Chaabihi, H., Devauchelle, G., Kaczorek, M.. and Lefranc, M.P. (1995) Design of cassette baculovirus vectors for the production of therapeutic antibodies in insect cells. Immunotechnology, 1, 189-196.
34. Raming, K., Krieger, J., Strotmann, J., Boekhoff, I., Kubick, S., Baumstark, C. and Breer, H. (1993) Cloning and expression of odorant receptors. Nature, 361, 353-356.
35. Reed, R. (1998) Opening the window to odor space. Science, 279, 193.
36. Riegel, C.I., Lanner-Herrera, C. and Slavicek, J.M. (1994) Identification and characterization of the ecdysteroid UDP-glucosyltransferase gene of the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus. J. Gen. Virol., 75, 829-838.
37. Rouquier, S., Taviaux, S., Trask, B.J., Brand-Arpon, V., Van den Engh, G., Demaille, J. and Giorgi, D. (1998) Distribution of olfactory receptor genes in the human genome. Nat. Genet., 18, 243-250.
38. Sharon, D., Glusman, G., Pilpel, Y., Khen, M., Gruetzner, F., Haaf, T. and Lancet, D. (1999) Primate evolution of an olfactory receptor cluster: Diversification by gene conversion and recent emergence of pseudogenes. Genomics, 61, 24-36.
39. Vanti, W.B., Nguyen, T., Cheng, R., Lynch, K.R., George, S.R. and O'Dowd B.F. (2003) Novel human G-protein-coupled receptors. Biochem. Biophys. Res. Commun., 23, 67-71.
40. Volz, A., Ehlers, A., Younger, R., Forbes, S., Trowsdale, J., Schnorr, D., Beck, S. and Ziegler, A. (2003) Complex transcription and splicing of odorant receptor genes. J. Biol. Chem., 278, 19691-19701.
41. Wetzel, C.H., Oles, M., Wellerdieck, C., Kuckkowiak, M., Gisselmann, G. and Hatt, H. (1999) Specificity and sensitivity of a human olfactory receptor functionally expressed in human embryonic kidney 293 cells and Xenopus laevis oocytes. J. Neurosci., 19, 7426-7433.
42. Zozulya, S., Echeverri, F. and Nguyen, T. (2001) The human olfactory repertoire. Genome Biol., 2, 18.1-18.12.