Efficient expression, purification, and characterization of C-terminally tagged, recombinant human asparagine synthetase

Archives of Biochemistry and Biophysics

Volumn 440, Issue 1, 2005, Pages 18-27

  Ciustea, Mihai ^a   Gutierrez, Jemy A ^a   Abbatiello, Susan E ^a   Eyler, John R ^a   Richards, Nigel G J ^a  

^a University of Florida ^*  (United States)

Author keywords

Acute lymphoblastic leukemia; Asparagine synthetase; Baculovirus; Fourier transform ion cyclotron resonance mass spectrometry; Inhibitor discovery; Protein expression; Proteomics

Indexed keywords

ASPARTATE AMMONIA LIGASE; RECOMBINANT ENZYME;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTROLLED STUDY; ENZYME ANALYSIS; HUMAN; KINETICS; MAMMAL; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; WILD TYPE;

AMINO ACID SEQUENCE; ASPARTATE-AMMONIA LIGASE; CATALYSIS; DRUG DESIGN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME INHIBITORS; HUMANS; KINETICS; LEUKEMIA, LYMPHOCYTIC, ACUTE; MOLECULAR SEQUENCE DATA; MUTATION; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; T-LYMPHOCYTES; TUMOR CELLS, CULTURED;

MAMMALIA;

EID: 22744438689     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.abb.2005.05.023     Document Type: Article

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