De novo alanine synthesis by bacteroids of Mesorhizobium loti is not required for nitrogen transfer in the determinate nodules of Lotus corniculatus

Journal of Bacteriology

Volumn 187, Issue 15, 2005, Pages 5493-5495

  Kumar, Shalini ^a   Bourdès, Alexandre ^a   Poole, Philip ^a  

^a UNIVERSITY OF READING   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; ALANINE DEHYDROGENASE;

ALDA GENE; AMINO ACID SYNTHESIS; AMINO ACID TRANSPORT; ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; BACTEROIDACEAE; ENZYME ACTIVITY; GENE DELETION; LOTUS; LOTUS CORNICULATUS; MESORHIZOBIUM; MESORHIZOBIUM LOTI; NITROGEN DYNAMICS; NITROGEN TRANSFER; NODULATION; NONHUMAN; PRIORITY JOURNAL; SYMBIOSIS;

ALANINE; ALANINE DEHYDROGENASE; AMINO ACID OXIDOREDUCTASES; BACTEROIDES; CULTURE MEDIA; GENE DELETION; GENES, BACTERIAL; LOTUS; NITROGEN; PLANT ROOTS; RHIZOBIUM;

LOTUS CORNICULATUS; MESORHIZOBIUM LOTI; PISUM SATIVUM;

EID: 22544466118     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.187.15.5493-5495.2005     Document Type: Article

References (21)

1. Allaway, D., E. Lodwig, L. A. Crompton, M. Wood, T. R. Parsons, T. Wheeler, and P. S. Poole. 2000. Identification of alanine dehydrogenase and its role in mixed secretion of ammonium and alanine by pea bacteroids. Mol. Microbiol. 36:508-515.
2. Appels, M. A., and H. Haaker. 1991. Glutamate oxaloacetate transaminase in pea root nodules-participation in a malate/aspartate shuttle between plant and bacteroid. Plant Physiol. 95:740-747.
3. Bergersen, F. J., and G. L. Turner. 1967. Nitrogen fixation by the bacteroid fraction of breis of soybean root nodules. Biochim. Biophys. Acta 141:507-515.
4. Brown, C. M., and M. J. Dilworth. 1975. Ammonia assimilation by Rhizobium cultures and bacteroids. J. Gen. Microbiol. 86:39-48.
5. Cullimore, J. V., and M. J. Bennett. 1991. Nitrogen assimilation in the legume root nodule: current status of the molecular biology of the plant enzymes. Can. J. Microbiol. 38:461-466.
6. Fellay, R., J. Frey, and H. Krisch. 1987. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. Gene 52:147-154.
7. Horton, R. M., H. D. Hunt, S. N. Ho, J. K. Pullen, and L. R. Pease. 1989. Engineering hybrid genes without the use of restriction enzymes-gene-splicing by overlap extension. Gene 77:61-68.
8. Kaneko, T., Y. Nakamura, S. Sato, E. Asamizu, T. Kato, S. Sasamoto, A. Watanabe, K. Idesawa, A. Ishikawa, K. Kawashima, T. Kimura, Y. Kishida, C. Kiyokawa, M. Kohara, M. Matsumoto, A. Matsuno, Y. Mochizuki, S. Nakayama, N. Nakazaki, S. Shimpo, M. Sugimoto, C. Takeuchi, M. Yamada, and S. Tabata. 2000. Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti. DNA Res. 7:331-338.
9. Kurz, W. G. W., D. A. Rokosh, and T. A. LaRue. 1975. Enzymes of ammonia assimilation in Rhizobium leguminosarum bacteroids. Can. J. Microbiol. 21: 1009-1012.
10. 2 fixation by bacteroids from soybean root nodules. Microbiology 148:1959-1966.
11. Lodwig, E., S. Kumar, D. Allaway, A. Bourdès, J. Prell, U. Priefer, and P. Poole. 2004. Regulation of L-alanine dehydrogenase in Rhizobium leguminosarum bv. viciae and its role in pea nodules. J. Bacteriol. 186:842-849.
12. Lodwig, E. M., A. H. F. Hosie, A. Bourdes, K. Findlay, D. Allaway, R. Karunakaran, J. A. Downie, and P. S. Poole. 2003. Amino-acid cycling drives nitrogen fixation in the legume-Rhizobium symbiosis. Nature 422:722-726.
13. Poole, P. S., A. Blyth, C. J. Reid, and K. Walters. 1994. myo-Inositol catabolism and catabolite regulation in Rhizobium leguminosarum bv. viciae. Microbiology 140:2787-2795.
14. Quandt, J., and M. F. Hynes. 1993. Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria. Gene 127: 15-21.
15. Rosendahl, L., M. J. Dilworth, and A. R. Glenn. 1992. Exchange of metabolites across the peribacteroid membrane in pea root nodules. J. Plant Physiol. 139:635-638.
16. Smith, M. T., and D. W. Emerich. 1993. Alanine dehydrogenase from soybean nodule bacteroids-kinetic mechanism and pH studies. J. Biol. Chem. 268:10746-10753.
17. Smith, M. T., and D. W. Emerich. 1993. Alanine dehydrogenase from soybean nodule bacteroids: purification and properties. Arch. Biochem. Biophys. 304:379-385.
18. Streeter, J. G. 1989. Estimation of ammonium concentration in the cytosol of soybean nodules. Plant Physiol. 90:779-782.
19. Stripf, R., and D. Werner. 1978. Differentiation of Rhizobium japonicum. II. Enzymatic activities in bacteroids and plant cytoplasm during the development of nodules of Glycine max. Z. Naturforsch. Teil C 33:373-381.
20. Temple, S. J., C. P. Vance, and J. S. Gantt. 1998. Glutamate synthase and nitrogen assimilation. Trends Plant Sci. 3:51-56.
21. 2-fixing soybean nodule bacteroids. Proc. Natl. Acad. Sci. USA 95:12038-12042.