Regulation of L-Alanine Dehydrogenase in Rhizobium leguminosarum bv. viciae and Its Role in Pea Nodules

Journal of Bacteriology

Volumn 186, Issue 3, 2004, Pages 842-849

  Lodwig, Emma ^a   Kumar, Shalini ^a   Allaway, David ^a   Bourdes, Alex ^a   Prell, Jürgen ^b   Priefer, Ursula ^b   Poole, Philip ^a  

^a UNIVERSITY OF READING   (United Kingdom)

^b RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE DEHYDROGENASE; CARBOXYLIC ACID;

ARTICLE; AUTOREGULATION; BACTERIAL METABOLISM; ENZYME ACTIVITY; ENZYME REGULATION; ENZYME SYNTHESIS; GENE FUSION; GENE MUTATION; GENE OVEREXPRESSION; NITROGEN FIXATION; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PEA; PRIORITY JOURNAL; RHIZOBIUM LEGUMINOSARUM;

ALANINE; ALANINE DEHYDROGENASE; AMINO ACID OXIDOREDUCTASES; ENZYME INDUCTION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, REGULATOR; MUTATION; PEAS; RHIZOBIUM LEGUMINOSARUM; SYMBIOSIS;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PISUM SATIVUM; RHIZOBIUM; RHIZOBIUM LEGUMINOSARUM; RHIZOBIUM LEGUMINOSARUM BV. VICIAE;

EID: 1642459138     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.3.842-849.2004     Document Type: Article

References (40)

1. Allaway, D., E. Lodwig, L. A. Crompton, M. Wood, T. R. Parsons, T. Wheeler, and P. S. Poole. 2000. Identification of alanine dehydrogenase and its role in mixed secretion of ammonium and alanine by pea bacteroids. Mol. Microbiol. 36:508-515.
2. Bergersen, F. J., and G. L. Turner. 1967. Nitrogen fixation by the bacteroid fraction of breis of soybean root nodules. Biochim. Biophys. Acta 141:507-515.
3. Beringer, J. E. 1974. R factor transfer in Rhizobium leguminosarum. J. Gen. Microbiol. 84:188-198.
4. Boesten, B., J. Batut, and P. Boistard. 1998. DctBD-dependent and independent expression of the Sinorhizobium (Rhizobium) meliloti C4-dicarboxylate transport gene (dctA) during symbiosis. Mol. Plant-Microbe Interact. 11:878-886.
5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
6. Brown, C. M., and M. J. Dilworth. 1975. Ammonia assimilation by Rhizobium cultures and bacteroids. J. Gen. Microbiol. 86:39-48.
7. Cabanes, D., P. Boistard, and J. Batut. 2000. Symbiotic induction of pyruvate dehydrogenase genes from Sinorhizobium meliloti. Mol. Plant-Microbe Interact. 13:483-493.
8. Cullimore, J. V., and M. J. Bennett. 1991. Nitrogen assimilation in the legume root nodule: current status of the molecular biology of the plant enzymes. Can. J. Microbiol. 38:461-466.
9. DelVecchio, V. G., V. Kapatral, R. J. Redkar, G. Patra, C. Mujer, T. Los, N. Ivanova, I. Anderson, A. Bhattacharyya, A. Lykidis, G. Reznik, L. Jablonski, N. Larsen, M. D'Souza, A. Bernal, M. Mazur, E. Goltsman, E. Selkov, P. H. Elzer, S. Hagius, D. O'Callaghan, J.-J. Letesson, R. Haselkorn, N. Kyrpides, and R. Overbeek. 2002. The genome sequence of the facultative intracellular pathogen Brucella melitensis. Proc. Natl. Acad. Sci. USA 99:443-448.
10. Dilworth, M. J., and A. R. Glenn. 1982. Movements of ammonia in Rhizobium leguminosarum. J. Gen. Microbiol. 128:29-37.
11. +-dependent malic enzyme of Rhizobium meliloti is required for symbiotic nitrogen fixation. Mol. Microbiol. 7:865-873.
12. Galibert, F., T. M. Finan, S. R. Long, A. Puhler, P. Abola, F. Ampe, F. Barloy-Hubler, M. J. Barnett, A. Becker, P. Boistard, G. Bothe, M. Boutry, L. Bowser, J. Buhrmester, E. Cadieu, D. Capela, P. Chain, A. Cowie, R. W. Davis, S. Dreano, N. A. Federspiel, R. F. Fisher, S. Gloux, T. Godrie, A. Goffeau, B. Golding, J. Gouzy, M. Gurjal, I. Hernandez-Lucas, A. Hong, L. Huizar, R. W. Hyman, T. Jones, D. Kahn, M. L. Kahn, S. Kalman, D. H. Keating, E. Kiss, C. Komp, V. Lalaure, D. Masuy, C. Palm, M. C. Peck, T. M. Pohl, D. Portetelle, B. Purnelle, U. Ramsperger, R. Surzycki, P. Thebault, M. Vandenbol, F. J. Vorholter, S. Weidner, D. H. Wells, K. Wong, K. C. Yeh, and J. Batut. 2001. The composite genome of the legume symbiont Sinorhizobium meliloti. Science 293:668-672.
13. Goodner, B., G. Hinkle, S. Gattung, N. Miller, M. Blanchard, B. Qurollo, B. S. Goldman, Y. Cao, M. Askenazi, C. Halling, L. Mullin, K. Houmiel, J. Gordon, M. Vaudin, O. Iartchouk, A. Epp, F. Liu, C. Wollam, M. Allinger, D. Doughty, C. Scott, C. Lappas, B. Markelz, C. Flanagan, C. Crowell, J. Gurson, C. Lomo, C. Sear, G. Strub, C. Cielo, and S. Slater. 2001. Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58. Science 294:2323-2328.
14. Gottfert, M., S. Rothlisberger, C. Kundig, C. Beck, R. Marty, and H. Hennecke. 2001. Potential symbiosis-specific genes uncovered by sequencing a 410-kilobase DNA region of the Bradyrhizobium japonicum chromosome. J. Bacteriol. 183:1405-1412.
15. Johnston, A. W. B., and J. E. Beringer. 1975. Identification of the Rhizobium strains in pea root nodules using genetic markers. J. Gen. Microbiol. 87:343-350.
16. Kaneko, T., Y. Nakamura, S. Sato, E. Asamizu, T. Kato, S. Sasamoto, A. Watanabe, K. Idesawa, A. Ishikawa, K. Kawashima, T. Kimura, Y. Kishida, C. Kiyokawa, M. Kohara, M. Matsumoto, A. Matsuno, Y. Mochizuki, S. Nakayama, N. Nakazaki, S. Shimpo, M. Sugimoto, C. Takeuchi, M. Yamada, and S. Tabata. 2000. Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti. DNA Res. 7:331-338.
17. Kurz, W. G. W., D. A. Rokosh, and T. A. LaRue. 1975. Enzymes of ammonia assimilation in Rhizobium leguminosarum bacteroids. Can. J. Microbiol. 21:1009-1012.
18. 2 fixation by bacteroids from soybean root nodules. Microbiology 148:1959-1966.
19. Lodwig, E. M., A. H. F. Hosie, A. Bourdes, K. Findlay, D. Allaway, R. Karunakaran, J. A. Downie, and P. S. Poole. 2003. Amino-acid cycling drives nitrogen fixation in the legume-Rhizobium symbiosis. Nature 422:722-726.
20. Madhusudhan, K. T., K. L. Hester, V. Friend, and J. R. Sokatch. 1997. Transcriptional activation of the bkd operon of Pseudomonas putida by BkdR. J. Bacteriol. 179:1992-1997.
21. Madhusudhan, K. T., J. Luo, and J. R. Sokatch. 1999. In vitro transcriptional studies of the bkd operon of Pseudomonas putida: L-branched-chain amino acids and D-leucine are the inducers. J. Bacteriol. 181:2889-2894.
22. Mathew, E., J. Zhi, and M. Freundlich. 1996. Lrp is a direct repressor of the dad operon in Escherichia coli. J. Bacteriol. 178:7234-7240.
23. Mendoza, A., B. Valderrama, A. Leija, and J. Mora. 1998. NifA-dependent expression of glutamate dehydrogenase in Rhizobium etli modifies nitrogen partitioning during symbiosis. Mol. Plant-Microbe Interact. 11:83-90.
24. Poole, P. S., A. Blyth, C. J. Reid, and K. Walters. 1994. myo-Inositol catabolism and catabolite regulation in Rhizobium leguminosarum bv. viciae. Microbiology 140:2787-2795.
25. Poole, P. S., N. A. Schofield, C. J. Reid, E. M. Drew, and D. L. Walshaw. 1994. Identification of chromosomal genes located downstream of dctD that affect the requirement for calcium and the lipopolysaccharide layer of Rhizobium leguminosarum. Microbiology 140:2797-2809.
26. Prell, J., B. Boesten, P. Poole, and U. B. Priefer. 2002. The Rhizobium leguminosarum bv. vicia VF39 gamma aminobutyrate (GABA) aminotransferase gene (gabT) is induced by GABA and highly expressed in bacteroids. Microbiology 148:615-623.
27. Prentki, P., and H. M. Krisch. 1984. In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29:303-313.
28. Quandt, J., and M. F. Hynes. 1993. Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria. Gene 127:15-21.
29. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.
30. Schafer, A., A. Tauch, W. Jager, J. Kalinowski, G. Thierbach, and A. Puhler. 1994. Small mobilizable multipurpose cloning vectors derived from the Escherichia coli plasmids pk18 and pk19-selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69-73.
31. 15N nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectrometry. Plant Physiol. 131:367-378.
32. Smith, M. T., and D. W. Emerich. 1993. Alanine dehydrogenase from soybean nodule bacteroids-kinetic mechanism and pH studies. J. Biol. Chem. 268:10746-10753.
33. Smith, M. T., and D. W. Emerich. 1993. Alaninc dehydrogenase from soybean nodule bacteroids: purification and properties. Arch. Biochem. Biophys. 304:379-385.
34. Streeter, J. G. 1989. Estimation of ammonium concentration in the cytosol of soybean nodules. Plant Physiol. 90:779-782.
35. Temple, S. J., C. P. Vance, and J. S. Gantt. 1998. Glutamate synthase and nitrogen assimilation. Trends Plant Sci. 3:51-56.
36. 2-fixing soybean nodule bacteroids. Proc. Natl. Acad. Sci. USA 95:12038-12042.
37. Watson, R. J., and V. K. Rastogi. 1993. Cloning and nucleotide sequencing of Rhizobium meliloti aminotransferase genes: an aspartate aminotransferase required for symbiotic nitrogen fixation is atypical. J. Bacteriol. 175:1919-1928.
38. Weinstein, M., R. C. Roberts, and D. R. Helinski. 1992. A region of the broad-host-range plasmid RK2 causes stable in planta inheritance of plasmids in Rhizobium meliloti cells isolated from alfalfa root nodules. J. Bacteriol. 174:7486-7489.
39. Wild, J., and T. Klopotowski. 1981. D-Amino acid dehydrogenase of Escherichia coli K12: positive selection of mutants defective in the enzyme activity and localization of the structural gene. Mol. Gen. Genet. 181:373-378.
40. Willins, D. A., C. Ryan,J. V. Platko, and J. M. Calvo. 1991. Characterization of Lrp, an Escherichia coli regulatory protein that mediates a global response to leucine. J. Biol. Chem. 266:10768-10774.