Acetaldehyde disrupts tight junctions and adherens junctions in human colonic mucosa: Protection by EGF and L-glutamine

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 289, Issue 2 52-2, 2005, Pages

  Basuroy, S ^a   Sheth, P ^a   Mansbach, C M ^a,b   Rao, R K ^a  

^a UNIVERSITY OF TENNESSEE HEALTH SCIENCE CENTER   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

Alcohol; Barrier function; Epidermal growth factor; Human colon

Indexed keywords

ACETALDEHYDE; ALCOHOL; BETA CATENIN; DETERGENT; EPIDERMAL GROWTH FACTOR; GAP JUNCTION PROTEIN; GLUTAMINE; OCCLUDIN; PROTEIN TYROSINE PHOSPHATASE; UNCLASSIFIED DRUG; UVOMORULIN; ZONULA OCCLUDENS 1;

ALCOHOL OXIDATION; ARTICLE; CELL JUNCTION; COLON MUCOSA; COLON PROTECTION; DIGESTIVE SYSTEM CANCER; ENZYME ACTIVITY; FEMALE; HUMAN; HUMAN TISSUE; IMMUNOFLUORESCENCE; MALE; METABOLITE; PERMEABILITY BARRIER; PRIORITY JOURNAL; PROTECTION; PROTEIN PHOSPHORYLATION; TIGHT JUNCTION;

ACETALDEHYDE; ADHERENS JUNCTIONS; COLON; DETERGENTS; DRUG INTERACTIONS; EPIDERMAL GROWTH FACTOR; FEMALE; GLUTAMINE; HUMANS; INTESTINAL MUCOSA; MALE; PHOSPHORYLATION; SOLUBILITY; TIGHT JUNCTIONS; TYROSINE;

EID: 22544451542     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.00464.2004     Document Type: Article

References (30)

1. Anderson JM and van Italie CM. Tight junctions and the molecular basis for regulation of paracelular permeability. Am J Physiol Gastrointest Liver Physiol 269: G467-G475, 1995.
2. Atkinson KA and Rao RK. Role of tyrosine phosphorylation in acetaldehyde mediated disruption of tight junctions. Am J Physiol Gastrointest Liver Physiol 280: G1280-G1288, 2001.
3. Balsamo J, Leung Ernst H T, Zanin MKB, Hoffman S, and Lilien J. Regulated binding of a PTP1B-like phosphatase to N-cadherin: control of cadherin-mediated adhesion by dephosphorylation of β-catenin. J Cell Biol 134: 801-813, 1996.
4. Carpenter G and Cohen S. Epidermal growth factor. J Biol Chem 265: 7709-7712, 1990.
5. Cowin P and Burke D. Cytoskeleton-membrane interaction. Curr Opin Cell Biol 8: 56-65, 1996.
6. Homann N, Tillonen J, Meurman JH, Rintamaki H, Lindqvist C, Rautio M, Jousimies-Somer H, and Salaspuro M. Increased salivary acetaldehyde levels in heavy drinkers and smokers: a microbiological approach to oral cavity cancer. Carcinogenesis 21: 663-668, 2000.
7. Hulsken J, Birchmeier W, and Beherens J. E-cadherin and APC compete for the interaction with β-catenin and the cytoskeleton. J Cell Biol 127: 2061-2091, 1994.
8. Itoh M, Furuse M, Morita K, Kubota K, Saitou M, and Tsukita S. Direct binding of tight junction-associated MAGUKs, ZO-1, ZO-2 and ZO-3, with the COOH termini of claudins. J Cell Biol 147: 1351-1363, 1999.
9. Kale G, Naren AP, Sheth P, and Rao RK. Tyrosine phosphorylation of C-terminal tail of occludin prevents its interaction with ZO-1, ZO-2, ZO-3. Biochem Biophys Res Commun 302: 324-329, 2003.
10. Koivisto T and Salaspuro M. Aldehyde dehydrogenases of the rat colon: comparison with other tissues of the alimentary tract and the liver. Alcohol Clin Exp Res 20: 551-555, 1996.
11. Koivisto T and Salaspuro M. Effects of acetaldehyde on brush border enzyme activities in human colon adenocarcinoma cell line Caco-2. Alcohol Clin Exp Res 21: 1599-1605, 1997.
12. Ma TY, Hollander D, Tran LT, Nguyen D, Hoa N, and Bhalla D. Cytoskeletal regulation of Caco-2 intestinal monolayer paracellular permeability. J Cell Physiol 164: 533-545, 1995.
13. Matthews RJ, Cahir ED, Thomas ML. Identification of an additional member of the protein-tyrosine-phosphatase family: evidence for alternative splicing in the tyrosine phosphatase domain. Proc Natl Acad Sci USA 87: 4444-4448, 1990.
14. Nagafuchi A and Takeichi M. Transmembrane control of cadherin-mediated cytoplasmic domain of E-cadherin. Cell Regul 1: 37-44, 1989.
15. Poschl G and Seitz HK. Alcohol and cancer. Alcohol Alcohol 39: 155-165, 2004.
16. Rao RK. Biologically active peptides in the gastrointestinal lumen. Life Sci 48: 1685-1704, 1991.
17. Rao RK. Acetaldehyde-induced increase in paracellular permeability in Caco-2 cell monolayer. Alcohol Clin Exp Res 22: 1724-1730, 1998.
18. Rao RK, Rao VU, Karnaky KJ, and Gupta A. Tyrosine phosphorylation and dissociation of occludin/ZO-1 and E-cadherin/β-catenin complexes from the cytoskeleton by oxidative stress. Biochem J 368: 471-481, 2002.
19. Rao RK, Baker RD, and Baker SS. Epidermal growth factor delays oxidant-induced disruption of the intestinal epithelial barrier function. Biochem Pharmacol 57: 685-697, 1999.
20. Rao RK, Seth A, and Sheth P. Intestinal permeability and endotoxemia in alcoholic liver disease. Am J Physiol Gastrointest Liver Physiol 286: G881-G884, 2004.
21. Reeds PJ and Burrin DG. Glutamine and the bowel. J Nutr 131: 2505S-2508S, 2001.
22. Salaspuro M. Bacteriocolonic pathway for ethanol oxidation: characteristics and implications. Ann Med 28: 195-200, 1996.
23. Salaspuro M. Acetaldehyde, microbes, and cancer of the digestive tract. Crit Rev Clin Lab Sci 40: 183-208, 2003.
24. Seitz HK, Poschl G, and Simanoski US. Alcohol and cancer. Recent Dev Alcohol 14: 67-95, 1998.
25. Seth A, Sheth P, Basuroy S, and Rao RK. L-Glutamine ameliorates acetaldehyde-induced paracellular permeability in Caco-2 cell monolayer. Am J Physiol Gastrointest Liver Physiol 287: G510-G517, 2004.
26. Sheth P, Seth A, Thangavel M, Basuroy S, and Rao RK. Epidermal growth factor prevents acetaldehyde-induced disruption of tight junctions in Caco-2 cell monolayer. Alcohol Clin Exp Res 28: 797-804, 2004.
27. Tillonen J, Vakevainen S, Salaspuro V, Zhang Y, Rautio M, Jousimies-Somer H, Kindros K, and Salaspuro M. Metronidazole increases intracolonic but not peripheral blood acetaldehyde in chronic ethanol-treated rats. Alcohol Clin Exp Res 24: 570-575, 2000.
28. Tsukita S and Furuse M. Occludin and claudins in tight junction strands: leading or supporting players? Trends Cell Biol 9: 268-273, 1999.
29. Visapaa JP, Tillonen J, and Salaspuro M. Microbes and mucosa in the regulation of intracolonic concentration during ethanol challenge. Alcohol Alcohol 37: 322-326, 2002.
30. Wittchen ES, Haskins J, and Stevenson BR. Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3. J Biol Chem 49: 35179-35185, 1999.