A large-scale automated method for hepatocyte isolation: Effects on proliferation in culture

Cell Transplantation

Volumn 14, Issue 5, 2005, Pages 291-299

  Nieuwoudt, M J ^a   Kreft, E ^a   Olivier, B ^a   Malfeld, S ^b   Vosloo, J ^c   Stegman, F ^a   Kunneke, R ^a   Van Wyk, A J ^a   Van Der Merwe, S W ^a  

^a UNIVERSITY OF PRETORIA   (South Africa)

^b NATIONAL HEALTH LABORATORY SERVICE   (South Africa)

^c STEVE BIKO ACADEMIC HOSPITAL   (South Africa)

Author keywords

Bioartificial liver; Collagenase liver perfusion; Isolated hepatocytes; Liver cell isolation

Indexed keywords

ASPARTATE AMINOTRANSFERASE; DNA; LACTATE DEHYDROGENASE; LIDOCAINE;

ANIMAL CELL; ARTICLE; ARTIFICIAL LIVER; ARTIFICIAL ORGAN; AUTOMATION; CELL CYCLE; CELL PROLIFERATION; CELL TRANSPLANTATION; CELL VIABILITY; FLOW CYTOMETRY; HEPATOCYTE TRANSPLANTATION; LIVER CELL CULTURE; LIVER PERFUSION; MEDICAL INSTRUMENTATION; MEDICAL TECHNOLOGY; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; SWINE;

SUIDAE;

EID: 22544448091     PISSN: 09636897     EISSN: None     Source Type: Journal    
DOI: 10.3727/000000005783983007     Document Type: Article

References (14)

1. Falasca, L.; Michelli, A.; Sartori, E.; Tomassini, A.; Devirgiliis, L. C. Hepatocytes entrapped in alginate gel beads and cultured in bioreactor: Rapid repolarization and reconstitution of adhesion areas. Cells Tissues Organs 168:126-136; 2001.
2. Foy, B. D.; Lee, J.; Morgan, J.; Toner, M.; Tompkins, R. G.; Yarmush, M. L. Optimization of hepatocyte attachment to microcarriers: Importance of oxygen. Biotech. Bioeng. 42:579-588; 1993.
3. Gerlach, J. C.; Brombacher, J.; Kloppel, K.; Schnoy, N.; Neuhaus, P. Comparison of four methods for mass hepatocyte isolation from pig and human livers. Transplantation 57:1318-1322; 1994.
4. Gerlach, J. C.; Brombacher, J.; Smith, M.; Neuhaus, P. High yield isolation from pig livers for investigation of hybrid liver support systems: Influence of collagenase concentration and body weight. J. Surg. Res. 62:85-89; 1996.
5. Lecluyse, E. L.; Fix, J. A.; Audus, K. L.; Hochman, J. H. Regeneration and maintenance of bile canalicular networks in collagen-sandwiched hepatocytes. Toxicol. In Vitro 14:117-132; 2000.
6. Morsiani, E.; Brogli, M.; Galavotti, D.; Pazzi, P.; Puviani, A. C.; Azzena, G. F. Biologic liver support: Optimal cell source and mass. Int. J. Artif. Organs 25:985-993; 2002.
7. Morsiani, E.; Rozga, J.; Scott, H. C.; Lebow, L. T.; Moscioni, A. D.; Kong, L. B.; Mc Grath, M. F.; Demetriou, A. A. Automated liver cell processing facilitates large scale isolation and purification of porcine hepatocytes. ASAIO J. 41:155-161; 1995.
8. Nishikawa, M.; Uchino, J.; Matsushita, M.; Takahashi, M.; Taguchi, K.; Koike, M.; Kamachi, H.; Kon, H. Optimal oxygen tension conditions for functioning cultured hepatocytes in vitro. Artif. Organs 20(2):169-177; 1996.
9. Puviani, A. C.; Ottolenghi, C.; Tassinari, B.; Pazzi, P.; Morsiani, E. An update on high-yield isolation methods and on the potential clinical use of isolated liver cells. Comp. Biochem. Physiol. A 121:99-109; 1998.
10. Rotem, A.; Toner, M.; Bhatia, S.; Foy, B. D.; Tompkins, R. G.; Yarmush, M. L. Oxygen is a factor determining in vitro tissue assembly: Effects on attachment and spreading of hepatocytes. Biotech. Bioeng. 43:654-660; 1994.
11. Runge, D.; Kohler, C.; Kostrubsky, V. E.; Jager, D.; Lehman, T.; Runge, D. M.; May, U.; Stolz, D. B.; Strom, S. C.; Fleig, W. E.; Michalopoulos, G. K. Induction of cytochrome P450 CYP1A1, CYP1A2, and CYP3A4 but not of CYP2C9, CYP2C19, multidrug resistance associated protein (MDR-1) by prototypical inducers in human hepatocytes. Biochem. Biophys. Res. Commun. 273:333-341; 2000.
12. Sachs, D. H. The pig as a potential xenograft donor. Vet. Immunol. Immunopathol. 58:185-191; 1994.
13. Seglen, P. O. Preparation of rat liver cells. Methods Cell Biol. 13:29-34; 1976.
14. Talamini, M. A.; Kappus, B.; Hubbard, A. Repolarization of hepatocytes in culture. Hepatology 25(1):167-172; 1997.