Substrate preference is altered by mutations in the fifth transmembrane domain of Ptr2p, the di/tri-peptide transporter of Saccharomyces cerevisiae

Molecular Membrane Biology

Volumn 22, Issue 3, 2005, Pages 215-227

  Hauser, Melinda ^a   Kauffman, Sarah ^a   Naider, Fred ^b   Becker, Jeffrey M ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

^b CITY UNIVERSITY OF NEW YORK   (United States)

Author keywords

Peptide transport; Saccharomyces cerevisiae; Site directed mutagenesis; Substrate preference

Indexed keywords

CARBON 14; DIPEPTIDE; DNA; EPITOPE; FUNGAL PROTEIN; MEMBRANE PROTEIN; PROTEIN PTR2P; TRIPEPTIDE; TRITIUM; UNCLASSIFIED DRUG;

ARTICLE; CANDIDA ALBICANS; IMMUNOBLOTTING; NONHUMAN; PEPTIDE SYNTHESIS; PLASMID; PRIORITY JOURNAL; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; TOXICITY TESTING; YEAST CELL;

ALANINE; AMINO ACID SEQUENCE; BIOLOGICAL TRANSPORT; GENE EXPRESSION REGULATION, FUNGAL; HUMANS; LEUCINE; MEMBRANE TRANSPORT PROTEINS; METHIONINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN STRUCTURE, SECONDARY; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SUBSTRATE SPECIFICITY;

SACCHAROMYCES CEREVISIAE;

EID: 22544438743     PISSN: 09687688     EISSN: 14645203     Source Type: Journal    
DOI: 10.1080/09687860500093248     Document Type: Article

References (51)

1. Abramson J, Iwata S, Kaback HR. 2004. Lactose permease as a paradigm for membrane transport proteins. Mol Membr Biol 21:227-236.
2. Basrai MA, Zhang HL, Miller D, Naider F, Becker JM. 1992. Toxicity of oxalysine and oxalysine-containing peptides against Candida albicans: regulation of peptide transport by amino acids. J Gen Microbiol 138:2353-2362.
3. Becker JM, Naider F. 1977. Peptide transport in yeast: Uptake of radioactive trimethionine in Saccharomyces cerevisiae. Arch Biochem Biophys 178:245-255.
4. Bolger MB, Haworth IS, Yeung AK, Ann D, von Grafenstein H, Hamm-Alvarez S, Okamoto CT, Kim KJ, Basu SK, Wu S, Lee VH. 1998. Structure, function, and molecular modeling approaches to the study of the intestinal dipeptide transporter PepT1. J Pharmeceut Sci 87:1286-1291.
5. Boll M, Markovich D, Weber WM, Korte H, Daniel H, Murer H. 1994. Expression cloning of a cDNA from rabbit small intestine related to proton-coupled transport of peptides, beta-lactam antibiotics and ACE-inhibitors. Pflugers Arch 429:146-149.
6. Busch W, Saier MH, Jr. 2002. The transporter classification (TC) system, 2002. Grit Rev Biochem Mol Biol 37:287-337.
7. Chen XZ, Steel A, Hediger MA. 2000. Functional roles of histidine and tyrosine residues in the H(+)-peptide transporter PepT1. Biochem Biophys Res Comm 272:726-730.
8. Covitz KM, Amidon GL, Sadee W. 1998. Membrane topology of the human dipeptide transporter, hPEPT1, determined by epitope insertions. Biochemistry 37:15214-15221.
9. Daniel H, Kottra G. 2004. The proton oligopeptide cotransporter family SLC15 in physiology and pharmacology. Pflugers Arch 447:610-618.
10. David NE, Gee M, Andersen B, Naider F, Thorner J, Stevens RC. 1997. Expression and purification of the Saccharomyces cerevisiae alpha-factor receptor (Ste2p), a 7-transmembrane-segment G protein-coupled receptor. J Biol Chem 272:15553-15561.
11. Doring F, Theis S, Daniel H. 1997. Expression and functional characterization of the mammalian intestinal peptide transporter PepT1 in the methylotropic yeast Pichia pastoris. Biochem Biophys Res Comm 232:656-662.
12. Doring F, Michel T, Rosel A, Nickolaus M, Daniel H. 1998a. Expression of the mammalian renal peptide transporter PEPT2 in the yeast Pichia pastoris and applications of the yeast system for functional analysis. Mol Membr Biol 15:79-88.
13. Doring F, Walter J, Will J, Focking M, Boll M, Amasheh S, Clauss W, Daniel H. 1998b. Delta-aminolevulinic acid transport by intestinal and renal peptide transporters and its physiological and clinical implications. J Clin Invest 101:2761-2767.
14. Doring F, Will J, Amasheh S, Clauss W, Ahlbrecht H, Daniel H. 1998c. Minimal molecular determinants of substrates for recognition by the intestinal peptide transporter. J Biol Chem 273:23211-23218.
15. Fei YJ, Kanai Y, Nussberger S, Ganapathy V, Leibach FH, Romero MF, Singh SK, Boron WF, Hediger MA. 1994. Expression cloning of a mammalian proton-coupled oligopeptide transporter. Nature 368:563-566.
16. Fei YJ, Liu W, Prasad PD, Kekuda R, Oblak TG, Ganapathy V, Leibach FH. 1997. Identification of the histidyl residue obligatory for the catalytic activity of the human H+/peptide cotransporters PEPT1 and PEPT2. Biochemistry 36:452-460.
17. Foltz M, Meyer A, Theis S, Demuth HU, Daniel H. 2004. A rapid in vitro screening for delivery of peptide-derived peptidase inhibitors as potential drug candidates via epithelial peptide transporters. J Pharmacol Exp Therapeut 310:695-702.
18. Ganapathy ME, Brandsch M, Prasad PD, Ganapathy V, Leibach FH. 1995. Differential recognition of beta-lactam antibiotics by intestinal and renal peptide transporters, PEPT1 and PEPT2. J Biol Chem 270:25672-25677.
19. Ganapathy ME, Huang W, Wang H, Ganapathy V, Leibach FH. 1998. Valacyclovir: a substrate for the intestinal and renal peptide transporters PEPT1 and PEPT2. Biochem Biophys Res Comm 246:470-475.
20. Ganapathy V, Burckhardt G, Leibach FH. 1984. Characteristics of glycylsarcosine transport in rabbit intestinal brush-border membrane vesicles. J Biol Chem 259:8954-8959.
21. Gebauer S, Knutter I, Hartrodt B, Brandsch M, Neubert K, Thondorf I. 2003. Three-dimensional quantitative structure-activity relationship analyses of peptide substrates of the mammalian H(+)/peptide cotransporter PEPT1. J Medic Chem 46:5725-5734.
22. Gietz RD, Woods RA. 2002. Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol 350:87-96.
23. Hagting A, vd Velde J, Poolman B, Konings WN. 1997. Membrane topology of the di- and tripeptide transport protein of Lactococcus lactis. Biochemistry 36:6777-6785.
24. Hauser M, Donhardt AM, Barnes D, Naider F, Becker JM. 2000. Enkephalins are transported by a novel eukaryotic peptide uptake system. J Biol Chem 275:3037-3041.
25. Hauser M, Narita V, Donhardt AM, Naider F, Becker JM. 2001. Multiplicity and regulation of genes encoding peptide transporters in Saccharomyces cerevisiae. Mol Membr Biol 18:105-112.
26. Herrera-Ruiz D, Knipp GT. 2003. Current perspectives on established and putative mammalian oligopeptide transporters. J Pharmaceut Sci 92:691-714.
27. Island MD, Naider F, Becker JM. 1987. Regulation of dipeptide transport in Saccharomyces cerevisiae by micromolar amino acid concentrations. J Bacteriol 169:2132-2136.
28. Kaback HR, Sahin-Toth M, Weinglass AB. 2001. The kamikaze approach to membrane transport. Nature Rev Mol Cell Biol 2:610-620.
29. Knutter I, Theis S, Hartrodt B, Born I, Brandsch M, Daniel H, Neubert K. 2001. A novel inhibitor of the mammalian peptide transporter PEPT1. Biochemistry 40:4454-4458.
30. Knutter I, Hartrodt B, Theis S, Foltz M, Rastetter M, Daniel H, Neubert K, Brandsch M. 2004. Analysis of the transport properties of side chain modified dipeptides at the mammalian peptide transporter PEPT1. Eur J Pharmaceut Sci 21:61-67.
31. Kulkarni AA, Haworth IS, Lee VH. 2003a. Transmembrane segment 5 of the dipeptide transporter hPepT1 forms a part of the substrate translocation pathway. Biochem Biophys Res Comm 306:177-185.
32. Kulkarni AA, Haworth IS, Uchiyama T, Lee VH. 2003b. Analysis of transmembrane segment 7 of the dipeptide transporter hPepT1 by cysteine-scanning mutagenesis. J Biol Chem 278:51833-51840.
33. Kunkel TA, Roberts JD, Zakour RA. 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol 154:367-382.
34. Lin Cereghino GP, Lin Cereghino J, Sunga AJ, Johnson MA, Lim M, Gleeson MA, Cregg JM. 2001. New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris. Gene 263:159-169.
35. Meredith D. 2004. Site-directed mutation of arginine 282 to glutamate uncouples the movement of peptides and protons by the rabbit proton-peptide cotransporter PepT1. J Biol Chem 279:15795-15798.
36. Naider F, Becker JM, Katzir-Katchalski E. 1974. Utilization of methionine-containing peptides and their derivatives by a methionine-requiring auxotroph of Saccharomyces cerevisiae. J Biol Chem 249:9-20.
37. Naider F, Shenbagamurthi P, Steinfeld AS, Smith HA, Boney C, Becker JM. 1983. Synthesis and biological activity of tripeptidyl polyoxins as antifungal agents. Antimicrob Agents Chemother 24:787-796.
38. Oldenburg KR, Vo KT, Michaelis S, Paddon C. 1997. Recombination-mediated PCR-directed plasmid construction in vivo in yeast. Nucleic Acids Res 25:451-452.
39. Payne JW, Grail BM, Gupta S, Ladbury JE, Marshall NJ, O'Brien R, Payne GM. 2000. Structural basis for recognition of dipeptides by peptide transporters. Arch Biochem Biophys 384:9-23.
40. Perry JR, Basrai MA, Steiner HY, Naider F, Becker JM. 1994. Isolation and characterization of a Saccharomyces cerevisiae peptide transport gene. Mol Cell Biol 14:104-115.
41. Saier MH, Jr. 2000. A functional-phylogenetic classification system for transmembrane solute transporters. Microbiol Mol Biol Rev 64:354-411.
42. Saito H, Terada T, Okuda M, Sasaki S, Inui K. 1996. Molecular cloning and tissue distribution of rat peptide transporter PEPT2. Biochim Biophys Acta 1280:173-177.
43. Sikorski RS, Hieter P. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27.
44. Sugawara M, Huang W, Fei YJ, Leibach FH, Ganapathy V, Ganapathy ME. 2000. Transport of valganciclovir, a ganciclovir prodrug, via peptide transporters PEPT1 and PEPT2. J Pharmaceut Sci 89:781-789.
45. Terada T, Saito H, Mukai M, Inui K. 1997. Characterization of stably transfected kidney epithelial cell line expressing rat H+/ peptide cotransporter PEPT1: localization of PEPT1 and transport of beta-lactam antibiotics. J Pharmacol Exp Therapeut 281:1415-1421.
46. Terada T, Inui K. 2004. Peptide transporters: structure, function, regulation and application for drug delivery. Current Drug Metabol 5:85-94.
47. Theis S, Hartrodt B, Kottra G, Neubert K, Daniel H. 2002a. Denning minimal structural features in substrates of the H(+)/ peptide cotransporter PEPT2 using novel amino acid and dipeptide derivatives. Mol Pharmacol 61:214-221.
48. Theis S, Knutter I, Hartrodt B, Brandsch M, Kottra G, Neubert K, Daniel H. 2002b. Synthesis and characterization of high affinity inhibitors of the H+/peptide transporter PEPT2. J Biol Chem 277:7287-7292.
49. Uchiyama T, Kulkarni AA, Davies DL, Lee VH. 2003. Biophysical evidence for His57 as a proton-binding site in the mammalian intestinal transporter hPepT1. Pharmaceut Res 20:1911-1916.
50. Vieira J, Messing J. 1987. Production of single-stranded plasmid DNA. Methods Enzymol 153:3-11.
51. Yeung AK, Basu SK, Wu SK, Chu C, Okamoto CT, Hamm-Alvarez SF, von Grafenstein H, Shen WC, Kim KJ, Bolger MB, Haworth IS, Ann DK, Lee VH. 1998. Molecular identification of a role for tyrosine 167 in the function of the human intestinal proton-coupled dipeptide transporter (hPepT1). Biochem Biophys Res Comm 250:103-107.