Kinetic characterization of tetrapropylammonium inhibition reveals how ATP and Pi alter access to the Na+-K+-ATPase transport site

American Journal of Physiology - Cell Physiology

Volumn 289, Issue 2 58-2, 2005, Pages

  Gatto, Craig ^a   Helms, Jeff B ^a   Prasse, Megan C ^a   Arnett, Krista L ^b   Milanick, Mark A ^b  

^a ILLINOIS STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MISSOURI   (United States)

Author keywords

Dixon plot; Inorganic phosphate; P type adenosine triphosphatase; Quaternary ammonium

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ADENOSINE TRIPHOSPHATE; PHOSPHATE; POTASSIUM ION; TETRAPROPYLAMMONIUM;

ANIMAL CELL; ARTICLE; BINDING SITE; CONTROLLED STUDY; ENZYME KINETICS; ENZYME MECHANISM; EXTRACELLULAR SPACE; NONHUMAN; POTASSIUM TRANSPORT; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN TRANSPORT;

4-NITROPHENYLPHOSPHATASE; ADENOSINE TRIPHOSPHATE; ANIMALS; BINDING, COMPETITIVE; DOGS; KINETICS; MODELS, BIOLOGICAL; NA(+)-K(+)-EXCHANGING ATPASE; PHOSPHATES; POTASSIUM; QUATERNARY AMMONIUM COMPOUNDS; SODIUM;

EID: 22444436581     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.00043.2005     Document Type: Article

References (39)

1. Albers RW, Koval GJ, and Siegel GJ. Studies on the-interaction of ouabain and other cardio-active steroids with sodium-potassium-activated adenosine triphosphatase. Mol Pharmacol 4: 324-336, 1968.
2. Artigas P and Gadsby DC. Large diameter of palytoxin-induced Na/K pump channels and modulation of palytoxin interaction by Na/K pump ligands. J Gen Physiol 123: 357-376, 2004.
3. +-pump ligands modulate gating of palytoxin-induced ion channels. Proc Natl Acad Sci USA 100: 501-505, 2003.
4. +-ATPase by a monoclonal antibody. Biochemistry 25: 7155-7162, 1986.
5. +)-ATPase: ligand interactions and related enzyme forms. Biochim Biophys Acta 821: 17-29, 1985.
6. +-ATPase: enzyme conformations from ligands interactions and Rb occlusion experiments. Biochim Biophys Acta 940: 43-50, 1988.
7. i phosphorylation of Na,K-ATPase. Biochemistry 37: 16686-16696, 1998.
8. Costa CJ, Gatto C, and Kaplan JH. Interactions between Na,K-ATPase αsubunit ATP-binding domains. J Biol Chem 278: 9176-9184, 2003.
9. 3 cotransport in squid axons by quaternary ammonium ions. J Membr Biol 183: 25-32, 2001.
10. +-phosphatase sites studied with inside-out red cell membrane vesicles. J Biol Chem 255: 7827-7834, 1980.
11. i by their reactivity toward hydroxylamine and vanadate. Biochemistry 37: 13634-13642, 1998.
12. 86Rb from an occluded state of the Na,K-pump in the presence of ATP or ADP. J Biol Chem 262: 11104-11115, 1987.
13. i or vanadate. J Biol Chem 262: 11116-11127, 1987.
14. Forbush B III. The interaction of amines with the occluded state of the Na,K-pump. J Biol Chem 263: 7979-7988, 1988.
15. Gatto C and Milanick MA. Inhibition of the red blood cell calcium pump by eosin and other fluorescein analogues. Am J Physiol Cell Physiol 264: C1577-C1586, 1993.
16. Glynn IM, Hara Y, and Richards DE. The occlusion of sodium ions within the mammalian sodium-potassium pump: its role in sodium transport. J Physiol 351: 531-547, 1984.
17. Helms JB, Arnett KL, Gatto C, and Milanick MA. Bretylium, an organic quaternary amine, inhibits the Na,K-ATPase by binding to the extracellular K-site. Blood Cells Mol Dis 32: 394-400, 2004.
18. 32P from p-nitrophenyl phosphate into a microsomal phosphatase. Mol Pharmacol 6: 99-107, 1970.
19. +)-ATPase. IV. estimation of the purity and of the molecular weight and polypeptide content per enzyme unit in preparations from the outer medulla of rabbit kidney. Biochim Biophys Acta 356: 53-67, 1974.
20. +-ATPase. Methods Enzymol 156: 291-301, 1988.
21. Jorgensen PL, Håkansson KO, and Karlish SJD. Structure and mechanism of Na,K-ATPase: functional sites and their interactions. Annu Rev Physiol 65: 817-849, 2003.
22. Kaplan JH. Biochemistry of Na.K-ATPase. Annu Rev Biochem 71: 511-535, 2002.
23. +K)-ATPase following treatment with thimerosal. Arch Biochem Biophys 237: 386-395, 1985.
24. Kropp DL and Sachs JR. Kinetics of the inhibition of the Na-K pump by tetrapropylammonium chloride. J Physiol 264: 471-487, 1977.
25. Lowry OH, Rosebrough NJ, Farr AL, and Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-275, 1951.
26. + channels by tetraalkylammonium ions. FEBS Lett 554: 159-164, 2003.
27. Milanick MA, Arnett AL, Helms JB, Prasse M, and Gatto C. Differential size exclusion between the inside and outside facing cation sites of the Na,K-ATPase (Abstract). Biophys J 86: 495a, 2004.
28. Milanick MA and Arnett KL. Extracellular protons regulate the extracellular cation selectivity of the sodium pump. J Gen Physiol 120: 497-508, 2002.
29. Moczydlowski EG and Fortes PAG. Inhibition of sodium and potassium adenosine triphosphatase by 2′,3′-O-(2,4,6- trinitrocyclohexadienylidene) adenine nucleotides: implications for the structure and mechanism of the Na:K pump. J Biol Chem 256: 2357-2366, 1981.
30. Peluffo RD, Hara Y, and Berlin JR. Quaternary organic amines inhibit Na,K pump current in a voltage-dependent manner: direct evidence of an extracellular access channel in the Na,K-ATPase. J Gen Physiol 123: 249-263, 2004.
31. Post RL, Hegyvary C, and Kume S. Activation by adenosine triphosphate in the phosphorylation kinetics of sodium and potassium ion transport adenosine triphosphatase. J Biol Chem 247: 6530-6540, 1972.
32. +-dependent phosphatase. Biochim Biophys Acta 212: 509-511, 1970.
33. +)-dependent adenosine triphosphatase. Mol Pharmacol 1: 238-246, 1971.
34. Rossi RC, Kaufman SB, González Lebrero RM, Nørby JG, and Garrahan PJ. An attachment for nondestructive, fast quenching of samples in rapid-mixing experiments. Anal Biochem 270: 276-285, 1999.
35. Sachs JR and Conrad ME. Effect of tetraethylammonium on the active cation transport system of the red blood cell. Am J Physiol 215: 795-798, 1968.
36. +)-ATPase; stimulation and inhibition by substituted and unsubstituted amines. Biochim Biophys Acta 937: 161-176, 1988.
37. +-ATPase. Biochim Biophys Acta 982: 103-114, 1989.
38. +-stimulation of Na,K-ATPase by bretylium. Br J Pharmacol 104: 895-900, 1991.
39. +-ATPase. Biochim Biophys Acta 987: 75-82, 1989.