Effects of media composition on submerged culture spores of the entomopathogenic fungus, Metarhizium anisopliae var. acridum, Part 1: Comparison of cell wall characteristics and drying stability among three spore types

Biocontrol Science and Technology

Volumn 15, Issue 4, 2005, Pages 379-392

  Leland, Jarrod E ^a   Mullins, Donald E ^a   Vaughan, Larry J ^b   Warren, Herman L ^b  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

Author keywords

Biocontrol; Blastospores; Cell wall; Conidia; Media; Metarhizium anisopliae; Mycoinsecticide

Indexed keywords

BIOLOGICAL CONTROL; BIOPESTICIDE; ENTOMOPATHOGENIC ORGANISM; FUNGUS; SPORE;

FUNGI; METARHIZIUM ANISOPLIAE; METARHIZIUM ANISOPLIAE VAR. ACRIDUM; TRITICUM AESTIVUM;

EID: 22344437203     PISSN: 09583157     EISSN: None     Source Type: Journal    
DOI: 10.1080/09583150400016928     Document Type: Article

References (27)

1. Adamek L. 1963. Submerse cultivation of the fungus Metarhizium anisopliae (Metsch.). Folia Microbiologia (Praha) 10:255-257.
2. Bidochka MJ, Pfeifer TA, Khachatourians GG. 1987. Development of the entomopathogenic fungus, Beauveria bassiana in liquid cultures. Mycopathologia 99:77-83.
3. Bidochka MJ, St. Leger RJ, Joshi L, Roberts DW. 1995. The rodlet layer from aerial and submerged conidia of the entomopathogenic fungus Beauveria bassiana contains hydrophobin. Mycological Research 99:403-406.
4. Boucias DG, Pendland JC, Latge JP. 1988. Nonspecific factors involved in attachment of entomopathogenic Deuteromycetes to host insect cuticle. Applied and Environmental Microbiology 54:1795-1805.
5. Cliquet S, Jackson MA. 1997. Comparison of air-drying methods for evaluating the desiccation tolerance of liquid culture-produced blastospores of Paecilomyces fumosoroseus. World Journal of Microbiology Biotechnology 13:299-303.
6. Goettel MS, Inglis DG. 1997. Fungi: Hyphomycetes. In: Lacey L, editor. Manual of techniques in insect pathology. San Diego: Academic Press. pp 211-249.
7. Hajek AE. 1997. Ecology of terrestrial fungal entomopathogens. Advances in Microbial Ecology 15:193-249.
8. Hazen BW, Hazen KC. 1988. Modification and application of a simple, surface hydrophobicity detection method to immune cells. Journal of Immunological Methods 107:157-163.
9. Hedgecock S, Moore D, Higgins PM, Prior C. 1995. Influence of moisture content on temperature tolerance and storage of Metarhizium flavoviride conidia in an oil formulation. Biocontrol Science and Technology 5:371-377.
10. Hegedus DD, Bidochka MJ, Miranpuri GS, Khachatourians GG. 1992. A comparison of the virulence, stability and cell wall-surface characteristics of three spore types produced by the entomopathogenic fungus Beauveria bassiana. Applied Microbiology Biotechnology 36:785-789.
11. Jeffs LB, Xavier IJ, Matai RE, Khachatourians GG. 1999. Relationship between fungal spore morphologies and surface properties for entomopathogenic members of the genera Beauveria, Metarhizium, Paecilomyces, Tolypocladium, and Verticillium. Canadian Journal of Microbiology 45:936-948.
12. Jenkins NE, Goettel MS. 1997. Methods for mass-production of microbial control agents of grasshoppers and locusts. Memoirs of the Entomological Society of Canada 171:37-48.
13. Jenkins NE, Prior C. 1993. Growth and formation of true conidia by Metarhizium flavoviride in a simple liquid medium. Mycological Research 97:1489-1494.
14. Jenkins NE, Thomas MB. 1996. Effect of formulation and application method on the efficacy of aerial and submerged conidia of Metarhizium flavoviride for locust and grasshopper control. Pesticide Science 46:229-306.
15. Karnovsky MJ. 1965. A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. Journal of Cell Biology 27:137A.
16. Kassa A, Stephan D, Vidal S, Zimmerman G. 2004. Production and processing of Metarhizium anisopliae var. acridum submerged conidia for locust and grasshopper control. Mycological Research 108:93-100.
17. Kleespies RG, Zimmermann G. 1994. Viability and virulence of blastospores of Metarhizium anisopliae (Metch.) Sorokin after storage in various liquids at different temperature. Biocontrol Science and Technology 4:309-319.
18. Kleespies RG, Zimmerman G. 1998. Effect of additives on the production, viability and virulence of blastospores of Metarhizium anisopliae. Biocontrol Science and Technology 8:207-214.
19. Milner RJ, Huppatz RJ, Swaris SC. 1991. A new method for assessment of germination of Metarhizium conidia. Journal of Invertebrate Pathology 57:121-123.
20. Pendland JC, Boucias DG. 1984. Use of labeled lectins to investigate cell wall surfaces of the entomogenous hyphomycete Nomuraea rileyi. Mycopathologia 81:141-148.
21. Pendland JC, Boucias DG. 1986. Lectin binding characteristics of several entomogenous hyphomycetes: possible relationship to insect hemagglutinins. Mycologia 78:818-824.
22. Pendland JC, Hung S-Y, Boucias DG. 1993. Evasion of host defense by in vivo-produced protoplast-like cells of the insect mycopathogen Beauveria bassiana. Journal of Bacteriology 175:5962-5969.
23. Stephan D, Zimmerman G. 1998. Development of a spray-drying technique for submerged spores of entomopathogenic fungi. Biocontrol Science and Technology 8:3-11.
24. St. Leger RJ, Joshi L, Roberts D. 1998. Ambient pH is a major determinant in the expression of cuticle-degrading enzymes and hydrophobin by Metarhizium anisopliae. Applied and Environmental Microbiology 64:709-713.
25. St. Leger RJ, Butt TM, Goettel MS, Staples RC, Roberts DW. 1989. Production in vitro of appressoria by the entomopathogenic fungus Metarhizium anisopliae. Experimental Mycology 13:274-288.
26. Thomas KC, Khachatourians GG, Ingeldew WM. 1987. Production and properties of Beauveria bassiana conidia cultivated in submerged culture. Canadian Journal of Microbiology 33:12-20.
27. Zacharuk RY. 1970. Fine structure of the fungus Metarrhizium anisopliae infecting three species of the larval Elateridae (Coleoptera) I. Dormant and germinating conidia. Journal Invertebrate Pathology 15:63-80.