Validation of recombinant and bovine chymosin by mass spectrometry

Journal of Agricultural and Food Chemistry

Volumn 53, Issue 13, 2005, Pages 5230-5238

  Lilla, Sergio ^a   Mogna, Giovanni ^b   Addeo, Francesco ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b Mofin Alce   (Italy)

Author keywords

Chymosin; Electrospray quadrupole time of flight mass spectrometry; Peptide markers; Recombinant enzyme

Indexed keywords

CHYMOSIN; PEPTIDE FRAGMENT; RECOMBINANT PROTEIN; TRYPSIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CATTLE; CHEMISTRY; CLASSIFICATION; ELECTROSPRAY MASS SPECTROMETRY; GENETIC VARIABILITY; GENETICS; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CHYMOSIN; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRYPSIN; VARIATION (GENETICS);

BOVINAE;

EID: 22244493876     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf0478051     Document Type: Article

References (12)

1. Andren, A.; Bjorck, L. Milk-feeding maintains the prochymosin production in cells of bovine abomasal mucosa. Acta Physiol. Scand. 1986, 126, 419-427.
2. Lilla, S.; Caira, S.; Ferranti, P.; Addeo, F. Mass spectrometric characterisation of proteins in rennet and in chymosin-based milk-clotting preparations. Rapid Commun. Mass Spectrom. 2001, 15, 1101-1112.
3. Swinkels, B. W.; van Ooyen, A. J.; Bonekamp, F. J. The yeast Kluyveromyces lactis as an efficient host for heterologous gene expression. Antonie van Leeuwenhoek Int. J. G. 1993, 64, 187-201.
4. Collin, J. C.; Moulins, I.; Rolet-Répécaud, O.; Bailly, C.; Lagarde, G. Detection of recombinant chymosins in calf rennet by enzyme-linked immunoassorbent assay. Lait 1997, 77, 425-431.
5. Foltmann, B. Prochymosin and chymosin (prorennin and rennin). Biochem. J. 1969, 115, 3-4.
6. Pedersen, V. B.; Christensen, K. A.; Foltmann, B. Investigations on the activation of bovine prochymosin. Eur. J. Biochem. 1979, 94, 573-580.
7. Nielsen, P. K.; Foltmann, B. Purification and characterization of porcine pepsinogen B and pepsin B. Arch. Biochem. Biophys. 1995, 322, 417-422.
8. Wei, C.; Tang, B.; Zhang, Y.; Yang, K. Oxidative refolding of recombinant prochymosin. Biochem. J. 1999, 340, 345-351.
9. 210, in recombinant prochymosin (chymosin). Biochemistry 2000, 39, 12140-12148.
10. Yallorous, I.; Kappelhoff, R.; Schilling, O.; Wegmann, F.; Helms, M. W.; Auge, A.; Brachtendorf, G.; Grosse Berkhoff, E.; Beermann, B.; Hinz, H.-J.; Konig, S.; Peter-Katalinic, J.; Stocker, W. Activation mechanism of pro-astacin: role of the propeptide, tryptic and autoproteolytic cleavage and importance of precise amino-terminal processing. J. Mol. Biol. 2002, 324, 237-246.
11. Nagase, H. Activation mechanisms of matrix metalloproteinases. Biol. Chem. 1997, 378, 151-158.
12. Rozman, J.; Syojan, J.; Kuhelj, R.; Turk, V.; Turk, B. Autocatalytic processing of recombinant human procathepsin B is a bimolecular process. FEBS Lett. 1999, 459, 358-362.