Activation mechanism of pro-astacin: Role of the pro-peptide, tryptic and autoproteolytic cleavage and importance of precise amino-terminal processing

Journal of Molecular Biology

Volumn 324, Issue 2, 2002, Pages 237-246

  Yiallouros, Irene ^a   Kappelhoff, Reinhild ^a   Schilling, Oliver ^a   Wegmann, Frank ^a   Helms, Mike W ^a   Auge, Astrid ^a   Brachtendorf, Gertrud ^a   Berkhoff, Eva Große ^a   Beermann, Bernd ^a   Hinz, Hans Jürgen ^a   König, Simone ^a   Peter Katalinic, Jasna ^a   Stöcker, Walter ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Activation mechanism; Astacin; Metzincins; Pro enzyme; Zinc peptidase

Indexed keywords

ASTACIN; BREFELDIN A; MUTANT PROTEIN; PROTEIN DERIVATIVE; PROTEIN PRECURSOR; RECOMBINANT PROTEIN; SERYLPROLYLISOLEUCYLISOLEUCYLPROLYLGLUTAMYLALANYLALANYLARGINYLALANYLLEUCYLT RYOSYLTYROSYLASPARAGINYLASPARTYLGLYCYLMETHIONYLPHENYLALANYLGLUTAMYLGLYCYLASPARTY LISOLEUCYLLYSYLLEUCYLARGINYLALANYLGLYCYLARGINYLGLUTAMINYLPROLYLALANYLARGINYLVALY LGLYCINE; TRYPSIN; UNCLASSIFIED DRUG;

ACCURACY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ARTICLE; AUTOREGULATION; CHEMICAL BOND; CRAYFISH; IMMUNOPRECIPITATION; IN VITRO STUDY; IN VIVO STUDY; MASS SPECTROMETRY; MIDGUT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN STABILITY; THERMOSTABILITY;

ASTACOIDEA; ASTACUS; ASTACUS ASTACUS;

EID: 0036438912     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)01102-6     Document Type: Article

References (35)

1. Dumermuth, E., Sterchi, E. E., Jiang, W. P., Wolz, R. L., Bond, J. S., Flannery, A. V. & Beynon, R. J. (1991). The astacin family of metallo-endopeptidases. J. Biol. Chem. 266, 21381-21385.
2. Stöcker, W., Gomis-Rüth, F.-X., Bode, W. & Zwilling, R. (1993). Implications of the 3-dimensional structure of astacin for the structure and function of the astacin family of zinc-endopeptidases. Eur. J. Biochem. 214, 215-231.
3. Bond, J. S. & Beynon, R. J. (1995). The astacin family of metallo-endopeptidases. Protein Sci. 4, 1247-1261.
4. Bode, W., Gomis-Rüth, F.-X. & Stöcker, W. (1993). Astacins, serralysins, snake venom and matrix metalloproteinases exhibit identical zinc-binding environments (HEXXHXXGXXH and Met-turn) and topologies and should be grouped into a common family, the "metzincins". FEBS Letters, 331, 134-140.
5. Vogt, G., Stöcker, W., Storch, V. & Zwilling, R. (1989). Biosynthesis of Astacus protease, a digestive enzyme from crayfish. Histochemistry, 91, 373-381.
6. Kessler, E., Takahara, K., Biniaminov, L., Brusel, M. & Greenspan, D. S. (1996). Bone morphogenetic protein-1: The type I procollagen C-proteinase. Science, 271, 360-362.
7. Li, S. W., Sieron, A. L., Fertala, A., Hojima, Y., Arnold, W. V. & Prockop, D. J. (1996). The C-proteinase that processes procollagens to fibrillar collagens is identical to the protein previously identified as bone morphogenic protein-1. Proc. Natl Acad. Sci. USA, 93, 5127-5130.
8. Blader, P., Rastegar, S., Fischer, N. & Strähle, U. (1997). Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid. Science, 278, 1937-1940.
9. Scott, I. C., Blitz, I. L., Pappano, W. N., Imamura, Y., Clark, T. G., Steiglitz, B. M. et al. (1999). Mammalian BMP-1/tolloid-related metalloproteinases, including novel family member mammalian tolloid-like 2, have differential enzymatic activities and distributions of expression relevant to patterning and skeletogenesis. Dev. Biol. 213, 283-300.
10. Beynon, R. J., Shannon, J. D. & Bond, J. S. (1981). Purification and characterization of a metallo-endoproteinase from mouse kidney. Biochem. J. 199, 591-598.
11. Sterchi, E. E., Naim, H. Y., Lentze, M. J., Hauri, H. P. & Fransen, J. A. (1988). N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase: A metallo-endopeptidases of the human intestinal microvillus membrane which degrades biologically active peptides. Arch. Biochem. Biophys. 265, 105-118.
12. Köhler, D., Kruse, M., Stöcker, W. & Sterchi, E. E. (2000). Heterologously overexpressed, affinity-purified human meprin alpha is functionally active and cleaves components of the basement membrane in vitro. FEBS Letters, 465, 2-7.
13. Yasumasu, S., Yamada, K., Akasaka, K., Mitsunaga, K., Iuchi, I., Shimada, H. & Yamagami, K. (1992). Isolation of cDNAs for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, and concurrent expression of their mRNAs during development. Dev. Biol. 153, 250-258.
14. Geier, G. & Zwilling, R. (1998). Cloning and characterization of a cDNA coding for Astacus embryonic astacin, a member of the astacin family of metalloproteases from the crayfish Astacus astacus. Eur. J. Biochem. 253, 796-803.
15. Reyda, S., Jacob, E., Zwilling, R. & Stöcker, W. (1999). cDNA cloning, bacterial expression, in vitro renaturation and affinity purification of the zinc endopeptidase astacin. Biochem. J. 344, 851-857.
16. Geier, G., Jacob, E., Stöcker, W. & Zwilling, R. (1997). Genomic organization of the zinc-endopeptidase astacin. Arch. Biochem. Biophys. 337, 300-307.
17. Bode, W., Gomis-Rüth, F.-X., Huber, R., Zwilling, R. & Stöcker, W. (1992). Structure of astacin and implications for activation of astacins and zinc-ligation of collagenases. Nature, 358, 164-167.
18. Gomis-Rüth, F.-X., Stöcker, W., Huber, R., Zwilling, R. & Bode, W. (1993). Refined 1.8 Å X-ray crystal structure of astacin, a zinc-endopeptidase from the crayfish Astacus astacus L. Structure determination, refinement, molecular structure and comparison with thermolysin. J. Mol. Biol. 229, 945-968.
19. Stöcker, W. & Zwilling, R. (1995). Astacin. Methods Enzymol. 248, 305-325.
20. Wiederanders, B. (2000). The function of propeptide domains of cysteine proteinases. Advan. Expt. Med. Biol. 477, 261-270.
21. Yiallouros, I., Grosse Berkhoff, E. & Stöcker, W. (2000). The roles of Glu93 and Tyr149 in astacin-like zinc peptidases. FEBS Letters, 484, 224-228.
22. Möhrlen, F., Baus, S., Gruber, A., Rackwitz, H. R., Schnölzer, M., Vogt, G. & Zwilling, R. (2001). Activation of pro-astacin. Immunological and model peptide studies on the processing of immature astacin, a zinc-endopeptidase from the crayfish Astacus astacus. Eur. J. Biochem. 268, 2540-2546.
23. Anderson, E. D., Molloy, S. S., Jean, F., Fei, H., Shimamura, S. & Thomas, G. (2002). The ordered and compartment-specific autoproteolytic removal of the furin intramolecular chaperone is required for enzyme activation. J. Biol. Chem. 277, 12879-12890.
24. Sieron, A. L., Tretiakova, A., Jameson, B. A., Segall, M. L., Lund-Katz, S., Khan, M. T. et al. (2000). Structure and function of procollagen C-proteinase (mTolloid) domains determined by protease digestion, circular dichroism, binding to procollagen type I, and computer modeling. Biochemistry, 39, 3231-3239.
25. Stöcker, W., Ng, M. & Auld, D. S. (1990). Fluorescent oligopeptide substrates for kinetic characterization of the specificity of Astacus protease. Biochemistry, 29, 10418-10425.
26. Zwilling, R. & Neurath, H. (1981). Invertebrate proteases. Methods Enzymol. 80, 633-644.
27. Johnson, G. D. & Bond, J. S. (1997). Activation mechanism of meprins, members of the astacin metallo-endopeptidases family. J. Biol. Chem. 272, 28126-28132.
28. Nagase, H. (1997). Activation mechanisms of matrix metalloproteinases. Biol. Chem. 378, 151-160.
29. Stöcker, W., Grams, F., Baumann, U., Reinemer, P., Gomis-Rüth, F.-X., McKay, D. B. & Bode, W. (1995). The metzincins - Topological and sequential relations between the astacins, adamalysins, serralysins, and matrixins (collagenases) define a superfamily of zinc-peptidases. Protein Sci. 4, 823-840.
30. Stöcker, W., Wolz, R. L., Zwilling, R., Strydom, D. J. & Auld, D. S. (1988). Astacus protease, a zinc metalloenzyme. Biochemistry, 27, 5026-5032.
31. Cantor, C. R. & Schimmel, P. R. (1980). Biophysical Chemistry, Freeman, San Francisco.
32. Görg, A., Postel, W., Domscheit, A. & Günther, S. (1988). Two-dimensional electrophoresis with immobilized pH gradients of leaf proteins from barley (Hordeum vulgare): Method, reproducibility and genetic aspects. Electrophoresis, 9, 681-692.
33. Bairoch, A. & Apweiler, R. (2000). The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000. Nucl. Acids Res. 28, 45-48.
34. Kraulis, P. J. (1991). MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallog. 24, 946-950.
35. Titani, K., Torff, H. J., Hormel, S., Kumar, S., Walsh, K. A., Rödl, J. et al. (1987). Amino acid sequence of a unique protease from the crayfish Astacus fluviatilis. Biochemistry, 26, 222-226.