Tight transcriptional regulation of foreign genes in insect cells using an ecdysone receptor-based inducible system

Protein Expression and Purification

Volumn 42, Issue 2, 2005, Pages 236-245

  Dai, Xiaojiang ^a   Willis, Leslie G ^a   Palli, Subba R ^b   Theilmann, David A ^a  

^a SUMMERLAND RESEARCH AND DEVELOPMENT CENTRE   (Canada)

^b UNIVERSITY OF KENTUCKY   (United States)

Author keywords

Baculovirus; Ecdysone receptor; Inducible expression; Stably transformed insect cells; VP16

Indexed keywords

DIPHTHERIA TOXIN; ECDYSONE RECEPTOR; STEROID RECEPTOR;

ANIMAL; ARTICLE; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENE TRANSFER; GENETICS; LARVA; LEPIDOPTERA; MOUSE; PHYSIOLOGY; PLASMID;

ANIMALS; DIPHTHERIA TOXIN; GENE EXPRESSION REGULATION; GENE TRANSFER TECHNIQUES; LARVA; LEPIDOPTERA; MICE; PLASMIDS; RECEPTORS, STEROID;

AUTOGRAPHA CALIFORNICA; CHORISTONEURA FUMIFERANA; CYPOVIRUS; HERPESVIRIDAE; HEXAPODA; INSECTA; LEPIDOPTERA; MAMMALIA; MUS MUSCULUS; NUCLEOPOLYHEDROVIRUS; ORGYIA PSEUDOTSUGATA; PICEA; SACCHAROMYCES CEREVISIAE;

EID: 22244453445     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.12.031     Document Type: Article

References (32)

1. L.M. Riddiford Hormone receptors and the regulation of insect metamorphosis Receptor 3 1993 203 209
2. M.R. Koelle, W.S. Talbot, W.A. Segraves, M.T. Bender, P. Cherbas, and D.S. Hogness The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily Cell 67 1991 59 77
3. H.E. Thomas, H.G. Stunnenberg, and A.F. Stewart Heterodimerization of the Drosophila ecdysone receptor with retinoid X receptor and ultraspiracle Nature 362 1993 471 475
4. T.P. Yao, W.A. Segraves, A.E. Oro, M. McKeown, and R.M. Evans Drosophila ultraspiracle modulates ecdysone receptor function via heterodimer formation Cell 71 1992 63 72
5. T.P. Yao, B.M. Forman, Z. Jiang, L. Cherbas, J.D. Chen, M. McKeown, P. Cherbas, and R.M. Evans Functional ecdysone receptor is the product of EcR and Ultraspiracle genes Nature 366 1993 476 479
6. K.S. Christopherson, M.R. Mark, V. Bajaj, and P.J. Godowski Ecdysteroid-dependent regulation of genes in mammalian cells by a Drosophila ecdysone receptor and chimeric transactivators Proc. Natl. Acad. Sci. USA 89 1992 6314 6318
7. D. No, T.P. Yao, and R.M. Evans Ecdysone-inducible gene expression in mammalian cells and transgenic mice Proc. Natl. Acad. Sci. USA 93 1996 3346 3351
8. S.R. Palli, M.Z. Kapitskaya, M.B. Kumar, and D.E. Cress Improved ecdysone receptor-based inducible gene regulation system Eur. J. Biochem. 270 2003 1308 1315
9. J. Stolarov, K. Chang, A. Reiner, L. Rodgers, G.J. Hannon, M.H. Wigler, and V. Mittal Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor Proc. Natl. Acad. Sci. USA 98 2001 13043 13048
10. S.T. Suhr, E.B. Gil, M.C. Senut, and F.H. Gage High level transactivation by a modified Bombyx ecdysone receptor in mammalian cells without exogenous retinoid X receptor Proc. Natl. Acad. Sci. USA 95 1998 7999 8004
11. M.B. Kumar, D.W. Potter, R.E. Hormann, A. Edwards, C.M. Tice, H.C. Smith, M.A. Dipietro, M. Polley, M. Lawless, and P.R. Wolohan Highly flexible ligand binding pocket of ecdysone receptor: a single amino acid change leads to discrimination between two groups of nonsteroidal ecdysone agonists J. Biol. Chem. 279 2004 27211 27218
12. X. Dai, L.G. Willis, I. Huijskens, S.R. Palli, and D.A. Theilmann The acidic activation domains of the baculovirus transactivators IE1 and IE0 are functional for transcriptional activation in both insect and mammalian cells J. Gen. Virol. 85 2004 573 582
13. X. Hu, L. Cherbas, and P. Cherbas Transcription activation by the ecdysone receptor (EcR/USP): identification of activation functions Mol. Endocrinol. 17 2003 716 731
14. M.B. Kumar, T. Fujimoto, D.W. Potter, Q. Deng, and S.R. Palli A single point mutation in ecdysone receptor leads to increased ligand specificity: implications for gene switch applications Proc. Natl. Acad. Sci. USA 99 2002 14710 14715
15. S. Tomita, Y. Kawai, S.D. Woo, M. Kamimura, K. Iwabuichi, and A.S. Imanishi Ecdysone-inducible foreign gene expression in stably-transformed lepidopteran insect cells In Vitro Cell Dev. Biol. Anim. 37 2001 564 571
16. H.T. Tran, H.B. Askari, S. Shaaban, L. Price, S.R. Palli, T.S. Dhadialla, G.R. Carlson, and T.R. Butt Reconstruction of ligand-dependent transactivation of Choristoneura fumiferana ecdysone receptor in yeast Mol. Endocrinol. 15 2001 1140 1153
17. J. Poels, A. Martinez, M. Suner, A.D. Loof, S. Dunbar, and J.V. Broeck Functional and comparative analysis of two distinct ecdysteroid-responsive gene expression constructs in Drosophila S2 cells Insect Biochem. Mol. Biol. 34 2004 451 458
18. I. Grad, A. Niedziela-Majka, M. Kochman, and A. Ozyhar Analysis of Usp DNA binding domain targeting reveals critical determinants of the ecdysone receptor complex interaction with the response element Eur. J. Biochem. 268 2001 3751 3758
19. A. Niedziela-Majka, M. Kochman, and A. Ozyhar Polarity of the ecdysone receptor complex interaction with the palindromic response element from the hsp27 gene promoter Eur. J. Biochem. 267 2000 507 519
20. J.F. Mouillet, V.C. Henrich, M. Lezzi, and M. Vogtli Differential control of gene activity by isoforms A, B1 and B2 of the Drosophila ecdysone receptor Eur. J. Biochem. 268 2001 1811 1819
21. M. Vogtli, E. Elke, M.O. Imhof, and M. Lezzi High level transactivation by the ecdysone receptor complex at the core recognition motif Nucleic Acids Res. 26 1998 2407 2414
22. T. Tsukuda, S. Carleton, S. Fotheringham, and W.K. Holloman Isolation and characterization of an autonomously replicating sequence from Ustilago maydis Mol. Cell. Biol. 8 1988 3703 3709
23. T.A. Pfeifer, D.D. Hegedus, T.A. Grigliatti, and D.A. Theilmann Baculovirus immediate-early promoter-mediated expression of the Zeocin resistance gene for use as a dominant selectable marker in dipteran and lepidopteran insect cell lines Gene 188 1997 183 190
24. J.L. Vaughn, R.H. Goodwin, G.J. Tompkins, and P. McCawley The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae) In Vitro 13 1977 213 217
25. R.H. Goodwin, G.J. Tompkins, and P. McCawley Gypsy moth cell lines divergent in viral susceptibility. I. Culture and identification In Vitro 14 1978 485 494
26. M.J. Campbell Lipofection reagents prepared by a simple ethanol injection technique Biotechniques 18 1995 1027 1032
27. J.R. Neuman, C.A. Morency, and K.O. Russian A novel rapid assay for chloramphenicol acetyltransferase gene expression Biotechniques 5 1987 589 590
28. D.A. Theilmann, L.G. Willis, B.J. Bosch, I.J. Forsythe, and Q. Li The baculovirus transcriptional transactivator ie0 produces multiple products by internal initiation of translation Virology 290 2001 211 223
29. M. Yamaizumi, E. Mekada, T. Uchida, and Y. Okada One molecule of diphtheria toxin fragment A introduced into a cell can kill the cell Cell 15 1978 245 250
30. R.K. Holmes Biology and molecular epidemiology of diphtheria toxin and the tox gene J. Infect. Dis. 181 Suppl. 1 2000 S156 S167
31. E.M. Valdizan, E.V. Loukianov, and S. Olsnes Induction of toxin sensitivity in insect cells by infection with baculovirus encoding diphtheria toxin receptor J. Biol. Chem. 270 1995 16879 16885
32. C.C. Tsai, H.Y. Kao, T.P. Yao, M. McKeown, and R.M. Evans SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development Mol. Cell 4 1999 175 186