Evaluation of the COBAS TaqMan 48 real-time PCR system for quantitation of hepatitis B virus DNA

Journal of Clinical Microbiology

Volumn 43, Issue 7, 2005, Pages 3504-3507

  Gordillo, Rafael M ^a   Gutiérrez, Juan ^a   Casal, Manuel ^a  

^a HOSPITAL UNIVERSITARIO REINA SOFÍA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA;

ARTICLE; HEPATITIS B; HEPATITIS B VIRUS; INTERMETHOD COMPARISON; LABORATORY TEST; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; VIRUS HEPATITIS; VIRUS LOAD;

DNA, VIRAL; HEPATITIS B; HEPATITIS B VIRUS; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; TAQ POLYMERASE; VIRAL LOAD;

HEPATITIS B VIRUS;

EID: 22144494688     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.7.3504-3507.2005     Document Type: Article

References (17)

1. Bablok, W., H. Passing, R. Bender, and B. Schneider. 1988. A general regression procedure for method transformation. J. Clin. Chem Clin Biochem. 26:783-790.
2. Becker-André, M., and K. Hahlbrock. 1989. Absolute mRNA quantification using the polymerase chain reaction. A novel approach by a PCR aided transcript titration assay PATTY. Nucleic Acids Res. 17:9437-9446.
3. Bland, J. M., and D. G. Altman. 1986. Stastical methods for assessing agreement between two methods of clinical measurement. Lancet 1:307-310.
4. Chen, T., J. M. Luk, S. T. Cheung, W. C. Yu, and S. T. Fan. 2000 Evaluation of quantitative PCR and branched-chain DNA assay for detection of hepatitis B virus DNA in sera from hepatocellular carcinoma and liver transplant patients. J. Clin. Microbiol. 38:1977-1980.
5. Costa, J. 2004. Reaction en cadena de la polimerasa (PCR) a tiempo real. Enferm. Infecc. Microbiol. Clin. 22:299-305.
6. Ganem, D., and A. M. Prince. 2004. Hepatitis B virus infection -Natural history and clinical consequences. N. Engl. J. Med. 350:1118-1129.
7. Gerken, G., J. Gomes, P. Lampertico, M. Colombo, T. Rolhaar, M. Trippler, and G. Colucci. 1998. Clinical evaluation and applications of the Amplicor HBV Monitor test, a quantitative HBV DNA PCR assay. J. Virol. Methods. 74:155-165.
8. Gibson, U. E., C. A. Heid, and P. M. Williams. 1996. A novel method for real time quantitative real-time PCR. Genome Res. 6:995-1001.
9. Hendricks, D. A., B. J. Store, B. S. Hoo, J. L. Kolberg, B. D. Irvine, P. D. Neuwald, M. S. Urdea, and R. P. Perrillo. 1995. Quantitation of HBV DNA in human serum using a branched DNA (bDNA) signal amplification assay. Am. J. Clin. Pathol. 104:537-546.
10. Kessler, H. H., E. Stelzl, E. Daghofer, B. I. Santner, E. Marth, H. Lackner, and R. E. Stauber. 2000. Semiautomated quantification of hepatitis B virus DNA in a routine diagnostic laboratory. Clin. Diagn. Lab. Immunol. 7:853-855.
11. Lee, W. 1997. Hepatitis B virus infection. N. Engl. J. Med. 337:1733-1745.
12. Lok, A, S., E. J. Heathcote, and J. H. Hoofnagle. 2001. Manegement of hepatitis B: 2000 -summary of a workshop. Gastroenterology 120:1828.
13. López, V. A., E. J. Bourne, M. W. Lutz, and L. D. Condreay. 2002. Assessment of the COBAS Amplicor HBV Monitor test for quantitation of serum hepatitis B virus DNA levels. J. Clin. Microbiol. 40:1972-1976.
14. Marin, I. J., M. Poljak, K. Seme, J. Meglic-Volkar, M. Maticic, G. Lesnicar, and V. Brinovec. 2001. Comparative evaluation of semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR test and manual microwell plate-based AMPLICOR HBV MONITOR test. J. Clin. Microbiol. 39:758-761.
15. Noborg, U., A. Gusdal, E. K. Pisa, A. Hedrum, and M. Lindh. 1999. Automated quantitative analysis of Hepatitis B virus DNA by using the Cobas Amplicor HBV Monitor test. J. Clin. Microbiol. 37:2793-2797.
16. Pas, S. D., E. Fries, R. A. De Man, A. D. M. E. Osterhaus, and H. G. M. Niesters. 2000. Development of a quantitative real-time detection assay for hepatitis B virus DNA and comparison with two commercial assays. J. Clin. Microbiol. 38:2897-2901.
17. Weis, J., H. Wu, B. Farrenkopf, T. Schultz, G. Song, S. Shah, and J. Siegel. 2004. Real-time TaqMan PCR detection and quantitation of HBV genotype A-G with use of an internal quantitation standard. J. Clin. Virol. 30:86-93.