Biochemical characterization of a novel thermostable glucose-1-phosphate thymidylyltransferase from Thermuscaldophilus: Probing the molecular basis for its unusual thermostability

Enzyme and Microbial Technology

Volumn 37, Issue 4, 2005, Pages 402-409

  Parajuli, Niranjan ^a   Basnet, Devi B ^a   Young, Soo Chung ^b   Hei, Chan Lee ^a   Liou, Kwangkyoung ^a   Jae, Kyung Sohng ^a  

^a SunMoon University   (South Korea)

^b GeneChem Inc   (South Korea)

Author keywords

Sheets; Codon usage; dTTP; Glucose 1 phosphate thymidylyltransferase; Thermostability; Thermus caldophilus GK24

Indexed keywords

AMINO ACIDS; CATALYSIS; GLUCOSE; HYDROGEN BONDS; HYDROPHOBICITY; OXIDATION; PHOSPHATES; THERMODYNAMIC STABILITY;

CATALYTIC ACTIVITY; DENATURANTS; GUANIDINE HYDROCHLORIDE; METAL IONS;

ENZYMES;

GLUCOSE 1 PHOSPHATE THYMIDYLTRANSFERASE; TRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME DEGRADATION; HYDROGEN BOND; MOLECULAR DYNAMICS; MOLECULAR MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; PH MEASUREMENT; THERMOSTABILITY; THERMUS CALDOPHILUS;

THERMUS CALDOPHILUS;

EID: 22044451203     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2005.02.024     Document Type: Article

References (26)

1. W. Blankenfeldt, M. Asuncion, J.S. Lam, and J.H. Naismith The structural basis of the catalytic mechanism and regulation of glucose-1-phosphate thymidylyltransferase (RmlA) EMBO J 19 2000 6652 6663
2. W.A. Barton, J. Lesniak, J.B. Biggins, P.D. Jeffrey, J. Jiang, K.R. Rajashankar, J.S. Thorson, and D.B. Nikolov Structure, mechanism and engineering of a nucleotidylyltransferase as a first step toward glycorandomization Nat Struct Biol 8 2001 545 551
3. 2+ J Biol Chem 277 2002 44214 44219
4. S. Zuccotti, D. Zanardic, C. Rosano, L. Sturla, M. Tonetti, and M. Bolognesi Kinetic and crystallographic analyses support a sequential-ordered bi bi catalytic mechanism for Escherichia coli glucose-1-phosphate thymidylyltransferase J Mol Biol 313 2001 831 843
5. M.F. Perutz, and H. Raidt Stereochemical basis of heat stability in bacterial ferredoxins and in haemoglobin A2 Nature 255 1975 256 259
6. P. Argos, M.G. Rossmann, U.M. Grau, H. Zuber, G. Frank, and J.D. Tratschin Thermal stability and protein structure Biochemistry 18 1979 5698 5703
7. T.E. Creighton Prote: structures and molecular properties 2nd ed. 1997 Freeman & Company New York
8. A.C. Gregory, Singer, and A.H. Donal Thermophilic prokaryotes have characteristic patters of codon usage, amino acid composition and nucleotide content Gene 23 2003 39 47
9. Q. Liu, Y. Feng, X. Zhao, H. Dong, and Q. Xue Synonymous codon usage bias in Qryza sativa Plant Sci 167 2004 101 105
10. P.G. Sudeep, A. Absar, and B.R. Mala A novel thermostable xylanase from Thermomonospora sp.: influence of additives on thermostability Bioresour Technol 78 2001 221 224
11. S. Kumar, C.J. Tsai, and R. Nussinov Factors enhancing protein thermostability Prot Eng 13 2000 179 191
12. G.D. Haki, and S.K. Rakshit Developments in industrially important thermostable enzymes: a review Bioresour Technol 89 2003 17 34
13. S. Chakravarty, and R. Varadarajan Elucidation of determinants of protein stability through genome sequence analysis FEBS Lett 470 2000 65 69
14. D.P. Kreil, and C.A. Ouzounis Identification of thermophilic species by the amino acid compositions deduced from their genomes Nucleic Acids Res 29 2001 1608 1615
15. L. Xiao, and B. Honig Electrostatic contributions to the stability of hyperthermophilic proteins J Mol Biol 289 1999 1435 1444
16. N. Parajuli, D.S. Lee, H.C. Lee, K. Liou, and J.K. Sohng Cloning, expression and characterization of glucose-1-phosphate thymidylyltransferase (strmlA) from Thermus caldophilus Biotechnol Lett 26 2004 437 442
17. Peden JF, 〈http://www.molbiol.ox.ac.uk/cu/〉 distributes the program Codon W, 1997.
18. D. Frishman, and P. Argos Incorporation of non-local interactions in protein secondary structure prediction from the amino acid sequence Protein Eng 9 1996 133 142
19. Sali, L. Potterton, F. Yuan, V.H. Vlijmen, and M. Karplus Evaluation of comparative protein modeling by MODELLER Proteins 23 1995 318 326
20. G.M. Morris, D.S. Goodsell, R. Huey, and A.J. Olson Distributed automated docking of flexible ligands to proteins: parallel applications of AutoDock 2.4 J Comput Aided Mol Des 10 1996 293 304
21. R. Fraczkiewicz, and W. Braun GETAREA program J Comp Chem 19 1998 319 333
22. P.J. Lao, and D.R. Forsdyke Thermophilic bacteria strictly obey Szybalski's transcription direction rule and politely purine-load RNAs with both adenine and guanine Genome Res 10 2000 228 236
23. M. Graninger, B. Kneidinger, K. Bruno, A. Scheberl, and P. Messner Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP-beta-l-rhamnose biosynthesis in the Gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155 Appl Environ Microbiol 68 2002 3708 3715
24. J. Wu, Y. Bian, B. Tang, X. Chen, P. Shen, and Z. Peng Cloning and analysis of WF146 protease, a novel thermophilic subtilisin-like protease with four inserted surface loops FEMS Microbiol Lett 230 2004 251 258
25. J.M. Hess, and R.M. Kelly Influence of polymolecular events on inactivation behavior of xylose isomerase from Thermotoga neapolitana 5068 Biotechnol Bioeng 62 1999 509 517
26. B.S. Berlett, and E.R. Stadtman Protein oxidation in aging, disease, and oxidative stress J Biol Chem 272 1997 20313 20316