Exposure of bronchial epithelial cells to whole cigarette smoke: Assessment of cellular responses

Alternatives to Laboratory Animals

Volumn 33, Issue 3, 2005, Pages 239-248

  Phillips, Jeremy ^a   Kluss, Bruno ^b   Richter, Audrey ^a   Massey, Eian D ^a  

^a BRITISH AMERICAN TOBACCO   (United Kingdom)

^b Borgwaldt Technik GmbH   (Germany)

Author keywords

Cigarette; Cytotoxicity; Smoke; Vapour

Indexed keywords

ACROLEIN; ALDEHYDE; BENZENE; BENZO[A]PYRENE; CARBON MONOXIDE; CIGARETTE SMOKE; FORMALDEHYDE; HYDROGEN CYANIDE; INTERLEUKIN 6; INTERSTITIAL COLLAGENASE; MESSENGER RNA; MUCIN; MUCIN 5AC;

AEROSOL; AIR; ARTICLE; BRONCHUS MUCOSA; CELL MEMBRANE; CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOKINE PRODUCTION; CYTOTOXICITY; GAS ANALYSIS; HUMAN; HUMAN CELL; LIQUID; ONCOGENE; PARTICULATE MATTER; PRIORITY JOURNAL; SMOKING AND SMOKING RELATED PHENOMENA; UPREGULATION; VAPOR; WEIGHT;

EID: 21844451174     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119290503300310     Document Type: Article

References (16)

1. Keith, C.H. & Tesh, P.G. (1965). Measurement of the total smoke issuing from a burning cigarette. Tobacco Science 9, 61-64.
2. Bombick, D.W., Ayres, P.M. & Doolittle, D. J. (1997). Cytotoxicity assessment of whole smoke and vapour phase of mainstream and sidestream cigarette smoke from three Kentucky reference cigarettes. Toxicological Methods 7, 177-190.
3. Ritter, D., Knebel, J.W. & Aufderheide, M. (2001). In vitro exposure of isolated cells to native gaseous compounds - development and validation of an optimized system for human lung cells. Experimental Toxicology and Pathology 53, 373-386.
4. Ritter, D., Knebel, J.W. & Aufderheide, M. (2003). Exposure of human lung cells to inhalable substances: a novel test strategy involving clean air exposure periods using whole diluted cigarette main-stream smoke. Inhalation Toxicolology 15, 67-84.
5. Aufderheide, M., Knebel, J.W. & Ritter, D. (2003). An improved in vitro model for testing the pulmonary toxicity of complex mixtures such as cigarette smoke. Experimental Toxicology and Pathology 55, 51-57.
6. Wolz, L., Krause, G., Scherer, G., Aufderheide, M. & Mohr, U. (2002). In vitro genotoxicity assay of side-stream smoke using a human bronchial epithelial cell line. Food and Chemical Toxicology 40, 845-850.
7. Jeffery, P.K. (1998). Structural and inflammatory changes in COPD: a comparison with asthma. Thorax 53, 129-136.
8. Takeyama, K., Jung, B., Shim, J.J., Burgel, P.R., Dao-Pick, T., Ueki, I.F., Protin, U., Kroschel, P. & Nadel, J.A., (2001). Activation of epidermal growth factor receptors is responsible for mucin synthesis induced by cigarette smoke. American Journal of Physiology - Lung Cell Molecular Physiology 28, L165-L172.
9. Rusznak, C., Mills, P.R., Devalia, J.L., Sapsford, R.J., Davies, R.J., & Lozewicz, S. (2000). Effect of cigarette smoke on the permeability and IL-1beta and sICAM-1 release from cultured human bronchial epithelial cells of never-smokers, smokers, and patients with chronic obstructive pulmonary disease. American Journal of Physiology - Lung Cell Molecular Physiology 2, 530-536.
10. Beisswenger, C., Platz, J., Seifart, C., Vogelmeier, C., & Bals, R. (2004). Exposure of differentiated airway epithelial cells to volatile smoke in vitro. Respiration 71, 402-409.
11. Richter, A., O'Donnell, R.A., Powell, R.M., Sanders, M.W., Holgate, S.T., Djukanovic, R. & Davies, D.E. (2002). Autocrine ligands for the epidermal growth factor receptor mediate interleukin-8 release from bronchial epithelial cells in response to cigarette smoke. American Journal of Physiology -Lung Cell Molecular Physiology 27, 85-90.
12. Hewson, C.A., Edbrooke, M.R. & Johnston, S.L. (2004). PMA induces the MUC5AC respiratory mucin in human bronchial epithelial cells, via PKC, EGF/TGF-alpha, Ras/Raf, MEK, ERK and Sp1-dependent mechanisms. Journal of Molecular Biology 344, 683-695.
13. Phillips, J., Williamson, J. & Massey, E.D. (2002). A method to expose mammalian cells in vitro to whole smoke at the air-liquid interface. Mutagenesis 17, 566.
14. Anon. (2001). Guidance Document on Using In Vitro Data to Estimate In Vivo Starting Doses for Acute Toxicity. NIH Publication No: 01-4500, 102pp. Washington, DC, USA: National Institutes of Health.
15. Putnam, K.P., Bombick, D.W. & Doolittle, D.J. (2002). Evaluation of eight in vitro assays for assessing the cytotoxicity of cigarette smoke condensate. Toxicology in Vitro 16, 599-607.
16. Fukano, Y., Ogura, M., Eguchi, K, Shibagaki, M. & Suzuki, M. (2004). Modified procedure of a direct in vitro exposure system for mammalian cells to whole cigarette smoke. Experimental Toxicology and Pathology 5, 317-323.