Development of a functional in vitro assay as a novel tool for the standardization of allergen extracts in the human system

Allergy: European Journal of Allergy and Clinical Immunology

Volumn 60, Issue 8, 2005, Pages 1021-1028

  Vogel, L ^a   Luttkopf D ^a   Hatahet, L ^a   Haustein, D ^a   Vieths, S ^a  

^a PAUL EHRLICH INSTITUT   (Germany)

Author keywords

Allergen extract; Biological activity; Mediator release; Serum IgE; Standardization

Indexed keywords

ALLERGEN; CALCIUM; COMPLEMENTARY DNA; IMMUNOGLOBULIN E; IMMUNOGLOBULIN E RECEPTOR;

ANIMAL CELL; ARTICLE; BASOPHIL; BIOASSAY; CALCIUM CELL LEVEL; CALCIUM MOBILIZATION; CONTROLLED STUDY; CROSS LINKING; FLUORESCENCE ACTIVATED CELL SORTING; FUNCTIONAL ASSESSMENT; GENETIC CODE; GENETIC TRANSFECTION; HUMAN; IMMUNOCHEMISTRY; IN VITRO STUDY; MAST CELL LEUKEMIA; MEDIATOR RELEASE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RAT; RECEPTOR AFFINITY; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; STANDARDIZATION;

ALLERGENS; ANIMALS; ANTIBODY SPECIFICITY; BASOPHILS; BIOLOGICAL ASSAY; CELL LINE; DNA, COMPLEMENTARY; HUMANS; IMMUNOHISTOCHEMISTRY; IMMUNOLOGIC TECHNIQUES; PROTEIN ISOFORMS; RATS; RECEPTORS, IGE; REFERENCE STANDARDS; TRANSFECTION;

EID: 21744462815     PISSN: 01054538     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1398-9995.2005.00803.x     Document Type: Article

References (35)

1. AAAAI. The use of standardized allergen extracts. J Allergy Clin Immunol 1997;99:583-586.
2. Schmid-Grendelmeier P, Crameri R. Recombinant allergens for skin testing. Int Arch Allergy Immunol 2001;125: 96-111.
3. Casanovas M, Marañón F, Bel I. Comparison of skin-prick test assay and reverse enzyme immunoassay competition (REINA-C) for biological activity of allergens. Clin Exp Allergy 1994;24:134-139.
4. Beezhold D, Pugh B, Liss G, Sussman G. Correlation of protein levels with skin prick test reactions in patients allergic to latex. J Allergy Clin Immunol 1996;98:1097-1102.
5. Bauer PC, Mitsch A, Costabel U. Optimale diagnostische Konzentrationen von Prick- und Intrakutantestlösungen verschiedener Baum- und Kräuterpollen sowie Schimmelpilze. Allergologie 1995;18:560-567.
6. Cohen-Dayag A, Schneider H, Pecht I. Variants of the mucosal mast cell line (RBL-2H3) deficient in a functional membrane glycoprotein. Immunobiology 1992;185:124-149.
7. Gilfillan AM, Kado-Fong H, Wiggan GA, Hakimi J, Kent U, Kochar JP. Conservation of signal transduction mechanisms via the human FcεRIα after transfection into a rat mast cell line, RBL 2H3. J Immunol 1992;149:2445-2451.
8. Karamloo F, Wangorsch A, Kasahara H, Davin LB, Haustein D, Lewis NG et al. Phenylcoumaran benzylic ether and isoflavonoid reductases are a new class of cross-reactive allergens in birch pollen, fruits and vegetables. Eur J Biochem 2001;268:5310-5320.
9. Taudou G, Varin-Blank N, Jouin H, Marchand F, Weyer A, Blank U. Expression of the alpha chain of human FcεRI in transfected rat basophilic leukemia cells: functional activation after sensitization with human mite-specific IgE. Int Arch Allergy Immunol 1993;100:34-350.
10. Lin AY, Devaux B, Green A, Sagerström C, Elliott JF, Davis MM. Expression of T cell antigen receptor heterodimers in a lipid-linked form. Science 1990;249:677-679.
11. Takebe Y, Seiki M, Fujisawa J, Hoy P, Yokota K, Arai K et al. SRα promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol Cell Biol 1988;8:466-472.
12. Kochan J, Pettine LF, Hakimi J, Kishi K, Kinet JP. Isolation of the gene coding for the α subunit of the human high affinity IgE receptor. Nucleic Acids Res 1988;16:3584.
13. Maekawa K, Imagawa N, Tanaka Y, Harada S. Determination of the sequence coding for the β subunit of the human high-affinity IgE receptor. FEBS Lett 1992;302:161-165.
14. Küster H, Thompson H, Kinet JP. Characterization and expression of the gene for the human Fc receptor γ subunit. Definition of a new gene family. J Biol Chem 1990;265:6448-6452.
15. Kinet JP, Metzger H, Hakimi J, Kochan J. A cDNA presumptively coding for the α subunit of the receptor with high affinity for immunoglobulin E. Biochemistry 1987;26:4605-4610.
16. Kinet JP, Blank U, Ra C, White K, Metzger H, Kochan J. Isolation and characterization of cDNAs coding for the β subunit of the high-affinity receptor for immunoglobulin E. Proc Natl Acad Sci USA 1988;85:6483-6487.
17. Blank U, Ra C, Miller L, White K, Metzger H, Kinet JP. Complete structure and expression in transfected cells of high affinity IgE receptor. Nature 1989;337:187-189.
18. Hoffmann A, Vieths S, Haustein D. Biologic allergen assay for in vivo test allergens with an in vitro model of the murine type I reaction. J Allergy Clin Immunol 1997;99:227-232.
19. Maeyama K, Hohman RJ, Metzger H, Beaven MA. Quantitative relationships between aggregation of IgE receptors, generation of intracellular signals, and histamine secretion in rat basophilic leukemia (2H3) cells. Enhanced responses with heavy water. J Biol Chem 1986;261:2583-2592.
20. Vogel L, Götz M, Haustein D. Characterization of components of the murine B cell receptor complex and their role in anti-Ig stimulation. Scand J Immunol 1997;45:287-293.
21. Hoffmann A, Jamin A, Fötisch K, May S, Aulepp H, Haustein D et al. Determination of the allergenic activity of birch pollen and apple prick test solutions by measurement of β-hexosaminidase release from RBL-2H3 cells. Comparison with classical methods in allergen standardization. Allergy 1999;54:446-454.
22. Wilson AP, Pullar CE, Camp AM, Helm BA. Human IgE mediates stimulus secretion coupling in rat basophilic leukemia cells transfected with the α chain of the human high-affinity receptor. Eur J Immunol 1993;23:240-244.
23. Butterfield JH, Weiler D, Dewald G, Gleich GJ. Establishment of an immature mast cell line from a patient with mast cell leukemia. Leuk Res 1988;12:345-355.
24. Nilsson G, Blom T, Kusche-Gullberg M, Kjellen L, Butterfield JH, Sundström C et al. Phenotypic characterization of the human mast-cell line HMC-1. Scand J Immunol 1994;39:489-498.
25. Blom T, Huang R, Aveskogh M, Nilsson K, Hellman L. Phenotypic characterization of KU812, a cell line identified as an immature human basophilic leukocyte. Eur J Immunol 1992;22:2025-2032.
26. Blom T, Nilsson G, Sundström C, Nilsson K, Hellman L. Characterization of a human basophil-like cell line (LAMA-84). Scand J Immunol 1996;44:54-61.
27. Almlöf I, Nilsson K, Johansson V, Åkerblom E, Slotte H, Ahlstedt S et al. Induction of basophilic differentiation in the human basophilic cell line KU812. Scand J Immunol 1988;28:293-300.
28. Kirshenbaum AS, Akin C, Wu Y, Rottem M, Goff JP, Beaven MA et al. Characterization of novel stem cell factor responsive human mast cell lines LAD-1 and 2 established from a patient with mast cell sarcoma/leukemia; activation following aggregation of FcεRI or FcγRI. Leuk Res 2003;27:677-682.
29. Hoffmann A, Kaul S, Fötisch K, Danz N, Lüttkopf D, Hatahet L et al. Mediator release assays based on human and murine IgE: potential and limitations in allergen standardisation. Arb Paul-Ehrlich Inst 2003;94:76-85.
30. Löwe J, Jardieu P, Vangorp K, Fei DTW. Allergen-induced histamine release in rat mast cells transfected with the α subunits of FcεRI. J Immunol Meth 1995;184:113-122.
31. Takagi K, Nakamura R, Teshima R, Sawada J. Application of human FcεRI α-chain-transfected RBL-2H3 cells for estimation of active serum IgE. Biol Pharmaceut Bull 2003;26:252-255.
32. Dibbern DA, Palmer GW, Williams PB, Bock SA, Dreskin SC. RBL cells expressing human FcεRI are a sensitive tool for exploring functional IgE-allergen interactions: studies with sera from peanut-sensitive patients. J Immunol Meth 2003;274:37-45.
33. Holowka D, Baird B. Antigen-mediated IgE receptor aggregation and signaling: a window on cell surface structure and dynamics. Annu Rev Biophys Biomol Struct 1996;25:79-112.
34. Van Ree R. The CREATE project: EU support for the improvement of allergen standardization in Europe. Allergy 2004;59:571-574.
35. Committee for Proprietary Medicinal Products. Note for guidance on allergen products. CPMP/BWP/243/96 (http:// www.emea.eu.int/pdfs/human/bwp/ 024396en.pdf).