Preliminary Comparison of Three LightCycler PCR Assays for the Detection of Herpes Simplex Virus in Swab Specimens

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 22, Issue 12, 2003, Pages 764-767

  Whiley, D M ^a,b   Syrmis, M W ^a,b   Mackay, I M ^a,b   Sloots, T P ^a,b,c  

^a ROYAL CHILDREN'S HOSPITAL   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c QUEENSLAND HEALTH   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA;

ADOLESCENT; ADULT; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC KIT; EVALUATION; FEMALE; HERPES SIMPLEX VIRUS; HERPES SIMPLEX VIRUS 1; HERPES SIMPLEX VIRUS 2; HUMAN; LABORATORY TEST; MALE; MELTING CURVE ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; TECHNIQUE; VIRUS DETECTION; VIRUS GENE; VIRUS IDENTIFICATION;

ADOLESCENT; ADULT; BASE SEQUENCE; COHORT STUDIES; DNA, VIRAL; HERPES SIMPLEX; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SAMPLING STUDIES; SENSITIVITY AND SPECIFICITY; SIMPLEXVIRUS; SKIN; SPECIMEN HANDLING;

EID: 0346992049     PISSN: 09349723     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10096-003-1031-2     Document Type: Article

References (10)

1. Madhavan HN, Priya K, Anand AR, Therese KL (1999) Detection of herpes simplex virus (HSV) genome using polymerase chain reaction (PCR) in clinical samples: comparison of PCR with standard laboratory methods for the detection of HSV. J Clin Virol 14:145-151
2. Burrows J, Nitsche A, Bayly B, Walker E, Higgins G, Kok T (2002) Detection and subtyping of herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture. BMC Microbiol 9:12
3. Espy MJ, Uhl JR, Mitchell PS, Thorvilson JN, Svien KA, Wold AD, Smith TF (2000) Diagnosis of herpes simplex virus infections in the clinical laboratory by LightCycler PCR. J Clin Microbiol 38:795-799
4. Schalasta G, Arents A, Schmid M, Braun RW, Enders G (2000) Fast and type-specific analysis of herpes simplex virus types 1 and 2 by rapid PCR and fluorescence melting curve analysis. Infection 28:85-91
5. Schaade L, Kockelkorn P, Ritter K, Kleines M (2001) Detection of cytomegalovirus DNA in human specimens by LightCycler PCR: melting point analysis is mandatory to detect virus strains with point mutations in the target sequence of the hybridization probes. J Clin Microbiol 39:3809
6. Yuan CC, Miley W, Waters D (2001) A quantification of human cells using an ERV-3 real time PCR assay. J Virol Methods 91:109-117
7. Read SJ, Jeffery KJ, Bangham CR (1997) Aseptic meningitis and encephalitis: the role of PCR in the diagnostic laboratory. J Clin Microbiol 35:691-696
8. Mackay IM, Metharom P, Sloots TP, Wei MQ (2001) Quantitative PCR-ELAHA for the determination of retroviral vector transduction efficiency. Mol Ther 3:801-808
9. Ryncarz AJ, Goddard J, Wald A, Huang ML, Roizman B, Corey L (1999) Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples. J Clin Microbiol 37:1941-1947
10. Stocher M, Leb V, Berg J (2003) A convenient approach to the generation of multiple internal control DNA for a panel of real-time PCR assays. J Virol Methods 108:1-8