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2CO (95:5, 1 L; 9:1, 1 L; 85:15, 1 L; 80:20, 1 L, 75:25, 1 L) to obtain five fractions. The active fraction 5 (1.5 g) was purified by reverse-phase column chromatography (ODS-A, RP-18; 70-230 mesh; YMC-Gel), eluting successively with an isocratic of aqueous MeOH (90%) to produce compound 4 (3.7 mg) as a colorless oil and compound 5 (6.2 mg) as a white powder.
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