Mass processing-an improved technique for protein identification with mass spectrometry data

Journal of Biomolecular Techniques

Volumn 15, Issue 4, 2004, Pages 230-237

  Henkin, Josh A ^a   Jennings, Mark E ^a   Matthews, Dwight E ^a   Vigoreaux, Jim O ^a  

^a UNIVERSITY OF VERMONT   (United States)

Author keywords

Drosophila; MALDI TOF; Peptide mass fingerprint; Protein gel electrophoresis

Indexed keywords

PROTEIN;

ARTICLE; CHEMISTRY; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DATABASE;

CHEMISTRY, ANALYTICAL; DATABASES, PROTEIN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 21644459889     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Goffeau A, Barrell BG, Bussey H, et al. Life with 6000 Genes. Science 1996;274:546-567.
2. The C. elegans Sequencing Consortium. Genome sequence of the nematode C. elegans: A platform for investigating biology. Science 1998;282:2012-2018.
3. The Arabidopsis Genome Initiative. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 2000;408:796-815.
4. Myers EW, Sutton GG, Delcher AL, et al. A wholegenome assembly of Drosophila Sci 2000;287: 2196-2204.
5. Venter JC, Adams MD, Myers EW, et al. The sequence of the human genome. Science 2001;291:1304-1351.
6. Henzel WJ, Stults JT, Watanabe C. Paper presented at the ASMS Conference, Seattle, WA, July 29-Aug. 2, 1989.
7. Henzel WJ, Billeci TM, Stults JT, Wong SC, Grimley C, Watanabe C. Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases. Proc Natl Acad Sci USA 1993;90:5011-5015.
8. James P, Quadroni M, Carafoli E, Gonnet G. Protein identification by mass profile fingerprinting. Biochem Biophys Res Commun 1993;195:58-64.
9. Mann M, Hojrup P, Roepstorff P. Use of mass spectrometric molecular weight information to identify proteins in sequence databases. Biol Mass Spect 1993;22: 338-345.
10. Pappin DJ, Hojrup P, Bleasby AJ. Rapid identification of proteins by peptide-mass fingerpint. Curr Biol 1993;3:327-332.
11. Yates JR, 3rd, Speicher S, Griffin PR, Hunkapiller T. Peptide mass maps: A highly informative approach to protein identification. Anal Biochem 1993;214:397-408.
12. Perkins DN, Pappin DJ, Creasy DM, Cottrell JS. Probability- based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 1999;20:3551-3567.
13. Moore JR, Dickinson MH, Vigoreaux JO, Maughan DW. The effect of removing the N-terminal extension of the Drosophila myosin regulatory light chain upon flight ability and the contractile dynamics of indirect flight muscle. Biophys J 2000;78:1431-1440.
14. Gharahdaghi F, Weinberg CR, Meagher DA, Imai BS, Mische SM. Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity. Electrophoresis 1999;20:601-605.
15. Vigoreaux JO. Genetics of the Drosophila flight muscle myofibril: A window into the biology of complex systems. Bioessays 2001;23:1047-1063.
16. Ashman K, Houthaeve T, Clayton JD, et al. The application of robotics and mass spectrometry to the characterization of the Drosophila melanogaster indirect flight muscle proteome. Lett Peptide Sci 1997;4:57-65.