Rapid isolation and sequence analysis of the beta-tubulin gene from Porphyra yezoensis (Rhodophyta)

Journal of Applied Phycology

Volumn 17, Issue 1, 2005, Pages 1-5

  Gong, Qianhong ^a   Han, Feng ^a   Dai, Jixun ^a   Liu, Hongquan ^a   Guan, Huashi ^a   Yu, Wengong ^a  

^a OCEAN UNIVERSITY OF CHINA   (China)

Author keywords

Beta tubulin; Intronless; Inverse PCR; Porphyra yezoensis; Rhodophyta; Sequence analysis

Indexed keywords

GENE EXPRESSION; PROTEIN; RED ALGA;

CHONDRUS; CHONDRUS CRISPUS; CYANIDIOSCHYZON MEROLAE; EUKARYOTA; FUNGI; PORPHYRA; PORPHYRA YEZOENSIS; RHODOPHYTA; VIRIDIPLANTAE;

EID: 21544462876     PISSN: 09218971     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10811-005-5950-4     Document Type: Article

References (20)

1. Cheney D, Metz B, Stiller JW (2001) Agrobacterium-mediated genetic transformation in the macroscopic marine red alga Porphyra yezoensis. J. Phycol. Suppl. 37: 11.
2. Cleveland DW, Sullivan KF (1985) Molecular biology and genetics of tubulin. Annu. Rev. Biochem. 54: 331-365.
3. Drukman S, Kavallaris M (2002) Microtubule alterations and resistance to tubulin-binding agents. Int. J. Oncol. 21: 621-628.
4. Duan J, Gorovsky MA (2002) Both carboxy-terminal tails of alpha-and beta-tubulin are essential, but either one will suffice. Curr. Biol. 12: 313-316.
5. Fukuda S, Kitade Y, Miyamoto H, Nagashima S, Takahashi S, Ohba T, Asada K, Kato I, Saga N (2003) Isolation and characterization of an elongation factor-1alpha gene in Porphyra yezoensis (Rhodophyta). J. Appl. Phycol. 15: 81-86.
6. Keeling PJ, Dean JA, Hink-Schauer C, Douglas SE, Maier UG., Mcfadden G.I (1999) The secondary endosymbiont of the cryptomonad Guillardia theta contains alpha-, beta-, and gamma-tubulin genes. Mol. Biol. Evol. 16: 1308-1313.
7. Kitade Y, Ootsuka S, Iitsuka O, Saga N. (2003) Effect of DMSO on PCR of Porphyra yezoensis (Rhodophta) gene. J. Appl. Phycol. 15: 555-557.
8. +-ATPase c subunit from a marine red alga Porphyra yezoensis. DNA Research 6: 307-312.
9. Liaud MF, Brandt U, Cerff R (1995) The marine red alga Chondrus crispus has a highly divergent beta-tubulin gene with a characteristic 5′ intron: Functional and evolutionary implications. Plant Mol. Biol. 29: 313-325.
10. Liu QY, Baldauf SI, Reith ME (1996) Elongation factor 1 alpha genes of the red alga Porphyra purpurea include a novel, developmentally specialized variant. Plant Mol. Biol. 31: 77-85.
11. Lluisma AO, Ragan MA (1998a) Cloning and characterization of a nuclear gene encoding a starch-branching enzyme from the marine red alga Gracilaria gracilis. Curr. Genet. 34: 105-111.
12. Lluisma AO, Ragan MA (1998b) Characterization of a galactose-1-phosphate uridyl transferase gene from the marine red alga Gracilaria gracilis. Curr. Genet. 34: 112-119.
13. MacKay RM, Gallant JW (1991) Beta-tubulin are encoded by at least four genes in the brown alga Ecotocarpus variabilis. Plant Mol. Biol. 17: 487-492.
14. MacKay RM, Gallant JW (2002) Beta-tubulin gene of Porphyra purpurea(Rhodophyta). J. Phycol. 38: 1020-1023.
15. Nikaido I, Asmizu E, Nakajima M, Nakzmura Y, Saga N, Tabata S (2000) Generation of 10,1543 expressed sequence tags from leafy gametophyte of a marine red alga Porphyra yezoensis. DNA Research 7: 223-227.
16. Nozaki H, Matsuzaki M, Takahara M., Misumi O, Kuroiwa H, Hasegawa M, Tadasu shin-I, Kohara Y, Ogasawara N, Kuroiwa T (2003) The phylogenetic position of red algae revealed by multiple nuclear genes from mitochondria-containing eukaryotes and an alternative hypothesis on origin of plastids. J. Mol. Evol. 56: 485-497.
17. Stiller JW, Hall BD (2002) Evolution of the RNA polymerase II C-terminal domain. Proc. natl Acad. Sci. USA 99: 6091-6096.
18. Trivinos-Lagos L, Ohmachi T, Albrightson C, Burns RG. Ennis HL, Chisholm RL (1993) The highly divergent alpha- and beta-tubulins from Dictyostelium discoideum are encoded by single genes. J. Cell Sci. 105: 903-911.
19. Youngblom J, Schloss JA, Silflow CD (1984) The two beta-tubulin genes of Chlamydomonas reinhardtii code for identical proteins. Mol. Cell. Biol. 4: 2686-2696.
20. Wang P, Sun Y, Li X, Zhang L, Li W, Wang Y (2004) Rapid isolation and functional analysis of promoter sequence of the nitrate reductase gene from Chlorella ellispoidea. J. Appl. Phycol. 16: 11-16.