Investigation of Protein Export in Bifidobacterium breve UCC2003

Applied and Environmental Microbiology

Volumn 69, Issue 12, 2003, Pages 6994-7001

  MacConaill, Laura E ^a,b   Fitzgerald, Gerald F ^a,b   Van Sinderen, Douwe ^a,b  

^a UNIVERSITY COLLEGE CORK   (Ireland)

^b UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELLS; GENES; PROTEINS;

BIFIDOBACTERIAL CELLS; MOLECULAR INTERACTIONS;

MICROBIOLOGY;

ABC TRANSPORTER; BACTERIAL ENZYME; HYBRID PROTEIN; NUCLEASE; PLASMID DNA; POLYPEPTIDE; BACTERIAL PROTEIN; MICROCOCCAL NUCLEASE; SIGNAL PEPTIDE;

MICROBIOLOGY;

ARTICLE; BACTERIUM CULTURE; BIFIDOBACTERIUM; BIFIDOBACTERIUM BREVE; GRAM POSITIVE BACTERIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN TRANSPORT; SEQUENCE HOMOLOGY; WESTERN BLOTTING; AMINO ACID SEQUENCE; BIOLOGY; CHEMISTRY; DNA SEQUENCE; GENETICS; METABOLISM; MOLECULAR GENETICS; SECRETION;

BACTERIA (MICROORGANISMS); BIFIDOBACTERIUM; BIFIDOBACTERIUM BREVE; LACTOCOCCUS; LACTOCOCCUS LACTIS; POSIBACTERIA; UNIDENTIFIED SHUTTLE VECTOR;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BIFIDOBACTERIUM; COMPUTATIONAL BIOLOGY; MICROCOCCAL NUCLEASE; MOLECULAR SEQUENCE DATA; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA;

EID: 2142798560     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.12.6994-7001.2003     Document Type: Article

References (56)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tools. J. Mol. Biol. 215:403-410.
2. Altschul, S. F., T. L. Madden, A. A. Schafer, J. Hang, Z. Hang, W. Miller, and D. J. Lipman. 1997. Gapped Blast and PSI-Blast: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
3. Banerjee, S., and J. N. Hansen. 1988. Structure and expression of a gene encoding the precursor of subtilin, a small protein antibiotic. J. Biol. Chem. 263:9508-9514.
4. Berks, B. C., F. Sargent, and T. Palmer. 2000. The Tat protein export pathway. Mol. Microbiol. 5:260-274.
5. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
6. Bolhuis, A., C. P. Broekhuizen, A. Sorokin, M. L. van Roosmalen, G. Venema, S. Bron, W. J. Quax, and J. M. van Dijl. 1998. SecDF of Bacillus subtilis, a molecular Siamese twin required for the efficient secretion of proteins. J. Biol. Chem. 273:21217-21224.
7. Chen, M., and V. Nagarajan. 1994. Effect of alteration of charged residues at the N termini of signal peptides on protein export in Bacillus subtilis. J. Bacteriol. 176:5796-5801.
8. Cserzo, M., E. Wallin, I. Simon, G. von Heijne, and A. Elofsson. 1997. Prediction of transmembrane alpha-helices in procariotic membrane proteins: the dense alignment surface method. Protein Eng. 10:673-676.
9. de Man, J., M. Rogosa, and M. E. Sharpe. 1960. A medium for the culture of lactobacilli. J. Appl. Bacteriol. 23:130-135.
10. de Vos, W. M., P. Vos, H. de Haards, and I. Boerrigter. 1989. Cloning and expression of the Lactococcus lactis subsp. cremoris SK11 gene encoding an extracellular serine protease. Gene 85:169-176.
11. Dunne, C. 2001. Adaptation of bacteria to the intestinal niche: probiotics and gut disorder. Inflamm. Bowel Dis. 7:136-145.
12. Ferrari, E., A. S. Jarnagin, and B. F. Schmidt. 1993. Commercial production of extracellular enzymes, p. 917-937. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and other gram-positive bacteria. American Society for Microbiology, Washington, D.C.
13. Gasson, M. J. 1983. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J. Bacteriol. 154:1-9.
14. Gennity, J., J. Goldstein, and M. Inouye. 1990. Signal peptide mutants of Escherichia coli. J. Bioenerg. Biomembr. 22:233-269.
15. Guarner, F., and J.-R. Malagelada. 2003. Gut flora in health and disease. Lancet 360:512-519.
16. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
17. Heins, J., J. Suniano, H. Taniuchi, and C. Anfinsen. 1967. Characterization of a nuclease produced by Staphylococcus aureus. J. Biol. Chem. 242:1016-1020.
18. Hoffman, C. S., and A. Wright. 1985. Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion. Proc. Natl. Acad. Sci. 82:5107-5111.
19. Hols, P., A. Baulard, D. Garmyn, B. Delplace, S. Hogan, and J. Delcour. 1992. Isolation and characterisation of genetic expression and secretion signals from Enterococcus faecalis through the use of broad-host-range α-amylase probe vectors. Gene 118:21-30.
20. Iwata, M., and T. Morishita. 1989. The presence of plasmids in Bifidobacterium breve. Lett. Appl. Microbiol. 9:165-168.
21. Kleerebezem, M., J. Boekhorst, R. van Krankenberg, D. Molenaar, O. P. Kuipers, R. Leer, R. Tarchini, S. A. Peters, H. M. Sandbrink, M. W. E. J. Fiers, W. Stiekema, R. M. Klein Lankhorst, P. A. Bron, S. M. Hoffer, M. N. N. Groot, R. Kerkhoven, M. de Vries, B. Ursing, W. M. de Vos, and R. J. Siezen. 2003. Complete genome sequence of Lactobacillus plantarum WCFS1. Proc. Natl. Acad. Sci. 100:1990-1995.
22. Lachica, R. V. F., C. Genigeorgis, and P. D. Hoeprich. 1971. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl. Microbiol. 21:585-587.
23. Le Loir, Y., A. Gruss, S. D. Ehrlich, and P. Langella. 1994. Direct screening of recombinants in gram-positive bacteria using the secreted staphylococcal nuclease as a reporter. J. Bacteriol. 176:5135-5139.
24. Le Loir, Y., S. Nouaille, J. Commissaire, L. Bretigny, A. Gruss, and P. Langella. 2001. Signal peptide and propeptide optimisation for heterologous protein secretion in Lactococcus lactis. Appl. Environ. Microbiol. 67:4119-4127.
25. Liebl, W., and F. Götz. 1986. Studies on lipase directed export of Escherichia coli β-lactamase in Staphylococcus carnosus. Mol. Gen. Genet. 204:166-173.
26. Liebl, W., A. J. Sinskey, and K. H. Scbleifer. 1992. Expression, secretion, and processing of a staphylococcal nuclease by Corynebacterium glutamicum. J. Bacteriol. 174:1854-1861.
27. Lim, E. M., J. Rauzier, J. Timm, G. Tooea, A. Murray, B. Gicquel, and D. Portnoi. 1995. Identification of Mycobacterium tuberculosis DNA sequences encoding exported proteins by using phoA gene fusions. J. Bacteriol. 177:59-65.
28. Lory, S. 1994. Leader peptidase of type IV prepilins and related proteins, p. 17-29. In G. von Heijne (ed.), Signal peptidases. R. G. Landes Company, Austin, Tex.
29. Manoil, C., and J. Beckwith. 1985. TnphoA: a transposon probe for protein export signals. Proc. Natl. Acad. Sci. 82:8129-8133.
30. Møller, P. L., F. Jørgensen, O. C. Hansen, S. M. Madsen, and P. Stougaard. 2001. Intra- and extracellular β-galactosidases from Bifidobacterium bifidum and B. infantis: molecular cloning, heterologous expression, and comparative characterization. Appl. Environ. Microbiol. 67:2276-2283.
31. Msadek, T., F. Kunst, and G. Rapoport. 1993. Two-component regulatory systems, p. 729-745. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and other gram-positive bacteria. American Society for Microbiology, Washington, D.C.
32. Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Int. J. Neural Syst. 8:581-599.
33. Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10:1-6.
34. O'Riordan, K. 1998. Studies on antimicrobial activity and genetic diversity of Bifidobacterium species: molecular characterisation of a 5.75 kb plasmid and a chromosomally encoded recA gene homologue from Bifidobacterium breve. Ph.D. thesis. National University of Ireland, Cork, Ireland.
35. Otto, R., R. G. Lageveen, H. Veldkamp, and W. N. Konings. 1982. Lactate efflux-induced electrical potential in membrane vesicles of Streptomyces cremoris. J. Bacteriol. 149:733-738.
36. Paik, S. H., A. Chakicheria, and J. N. Hansen. 1998. Identification and characterization of the structural and transporter genes for, and the chemical and biological properties of, sublancin 168, a novel lantibiotic produced by Bacillus subtilis 168. J. Biol. Chem. 273:23134-23142.
37. Pearce, B. J., Y. B. Yin, and H. R. Masure. 1993. Genetic identification of exported proteins in Streptococcus pneumoniae. Mol. Microbiol. 9:1037-1050.
38. Poquet, L., S. D. Ehrlich, and A. Gruss. 1998. An export-specific reporter designed for gram-positive bacteria: application to Lactococcus lactis. J. Bacteriol. 180:1904-1912.
39. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57:50-108.
40. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
41. Schell, M. A., M. Karmirantzou, B. Snel, D. Vilanova, B. Berger, G. Pessi, M.-C. Zwahlen, F. Desiere, P. Bork, M. Delley, R. D. Pridmore, and F. Arigoni. 2002. The genome sequence of Bifidobacterium longum reflects its adaptation the human gastrointestinal tract. Proc. Natl. Acad. Sci. 99:14422-14427.
42. Schultz, J., R. R. Copley, T. Doerks, C. P. Ponting, and P. Bork. 2000. SMART: a web based tool for the study of genetically mobile domains. Nucleic Acids Res. 28:231-234.
43. Schultz, J., F. Milpetz, P. Bork, and C. P. Ponting. 1998. SMART, a simple modular architecture research tool: identification of signalling domains. Proc. Natl. Acad. Sci. 95:5857-5864.
44. Scotti, P. A., M. L. Urbanus, J. Brunner, J. W. de Gier, G. von Heijne, C. van der Does, A. J. Driessen, B. Oudega, and J. Luirink. 2000. YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase. EMBO J. 19:542-549.
45. Simon, D., and A. Chopin. 1988. Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis. Biochimie 70:559-566.
46. Simonen, M., and I. Palva. 1993. Protein secretion in Bacillus species. Microbiol. Rev. 57:109-137.
47. Tjalsma, H., A. Bolhuis, J. D. Jongbloed, S. Bron, and J. M. van Dijl. 2000. Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome. Microbiol. Mol. Biol. Rev. 64:515-547.
48. Tjalsma, H., A. Bolhuis, M. L. van Roosmalen, T. Wiegert, W. Schumann, C. P. Broekhuizen, W. J. Quax, G. Venema, S. Bron, and J. M. van Dijl. 1998. Functional analysis of the secretory precursor processing machinery of Bacillus subtilis: identification of a eubacterial homolog of archaeal and eukaryotic signal peptidases. Genes Dev. 12:2318-2331.
49. Tusnady, G. E., and I. Simon. 1998. Principles governing amino acid composition of integral membrane proteins: applications to topology prediction. J. Mol. Biol. 283:489-506.
50. van Asseldonk, M., G. Rutten, M. Oteman, R. J. Siezen, W. M. de Vos, and G. Simons. 1990. Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subsp. lactis MG1363. Gene 95:155-160.
51. van Dijl, J. M. 1990. Protein export in Bacillus subtilis and Escherichia coli. Ph.D. thesis. Groningen University, Groningen, The Netherlands.
52. van Dijl, J. M., A. Bolhuis, H. Tjalsma, J. H. D. Jongbloed, A. de Jong, and S. Bron. 2001. Protein transport pathways in Bacillus subtilis: a genome-based road map, p. 337-355. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.). Bacillus subtilis and its closest relatives: from genes to cells. American Society for Microbiology, Washington, D.C.
53. van Dijl, J. M., P. G. Braun, C. Robinson, W. J. Quax, H. Antelmann, M. Hecker, J. Müller, H. Tjalsma, S. Bron, and J. D. H. Jongbloed. 2002. Functional genomic analysis of the Bacillus subtilis Tat pathway for protein secretion. J. Biotechnol. 98:243-254.
54. von Heijne, G. 1988. Transcending the impenetrable: how proteins come to terms with membranes. Biochim. Biophys. Acta 947:307-333.
55. Wu, L.-F., B. Ize, A. Chanal, Y. Quentin, and G. Fichant. 2000. Bacterial twin-arginine signal peptide-dependent protein translocation pathway: evolution and mechanism. J. Mol. Microbiol. Biotechnol. 2:170-189.
56. Yanouri, A., R. A. Daniel, J. Errington, and C. E. Buchanan. 1993. Cloning and sequencing of the cell division gene pbpB, which encodes penicillin-binding protein 2B in Bacillus subtilis. J. Bacteriol. 175:7604-7616.