A widely applicable, high-throughput TR-FRET assay for the measurement of kinase autophosphorylation: VEGFR-2 as a prototype

Journal of Biomolecular Screening

Volumn 8, Issue 4, 2003, Pages 447-452

  Moshinsky, Deborah J ^a   Ruslim, Lany ^a   Blake, Robert A ^a   Tang, Flora ^a,b  

^a SUGEN INC   (United States)

^b AMGEN INC   (United States)

Author keywords

Autophosphorylation; Kinase; TR FRET; VEGFR 2

Indexed keywords

2,3 DIHYDRO 2 OXO 3 (4,5,6,7 TETRAHYDRO 1H INDOL 2 YLMETHYLENE) 1H INDOLE 5 SULFONIC ACID DIMETHYLAMIDE; ANGIOGENESIS INHIBITOR; LEFLUNOMIDE; RECEPTOR BLOCKING AGENT; STAUROSPORINE; SUNITINIB; UNCLASSIFIED DRUG; VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR 2 INHIBITOR; VASCULOTROPIN RECEPTOR 2; ANTIBODY; ENZYME INHIBITOR; INDOLE DERIVATIVE; PEPTIDE; PHOSPHOTRANSFERASE; PYRROLE DERIVATIVE;

ANALYTIC METHOD; ANGIOGENESIS; ARTICLE; ASSAY; AUTOPHOSPHORYLATION; DOSE RESPONSE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH THROUGHPUT SCREENING; IC 50; MEASUREMENT; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; BIOTINYLATION; CHEMISTRY; DRUG ANTAGONISM; EVALUATION; FLUOROIMMUNOASSAY; IMMUNOLOGY; KINETICS; METABOLISM; METHODOLOGY; PHOSPHORYLATION;

ANTIBODIES; BIOTINYLATION; ENZYME INHIBITORS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUOROIMMUNOASSAY; INDOLES; KINETICS; PEPTIDES; PHOSPHORYLATION; PHOSPHOTRANSFERASES; PYRROLES; VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2;

EID: 2142721002     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/1087057103255282     Document Type: Article

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