Enzymatic assay for perfluoro-tagged metabolites of L-DOPA using crude lysate from E. coli transformed with pKKAADCII

Applied Microbiology and Biotechnology

Volumn 64, Issue 4, 2004, Pages 556-559

  Dingman, S ^a   Snyder Leiby, T ^b   Mack, D J ^a   Thomas, R ^c   Guo, C ^c  

^a MARIST COLLEGE   (United States)

^b STATE UNIVERSITY OF NEW YORK   (United States)

^c Inovatia Laboratories Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOPAMINE; LEVODOPA;

ENZYME ACTIVITY;

ARTICLE; CELL LYSATE; CHEMICAL BOND; CONTROLLED STUDY; ENZYME ASSAY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; STANDARDIZATION;

AROMATIC-L-AMINO-ACID DECARBOXYLASES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DOPAMINE; ESCHERICHIA COLI; FLUORINE; FLUORINE COMPOUNDS; ISOMERISM; LEVODOPA; MAGNETIC RESONANCE SPECTROSCOPY; TRANSFORMATION, BACTERIAL;

ESCHERICHIA COLI;

EID: 2142675181     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-003-1485-2     Document Type: Article

References (15)

1. Benkovic SJ, Hammes-Schiffer S (2003) A perspective on enzyme catalysis. Science 301:1196-1202
2. Debnath J, Husain PA, May SW (1992) Activation of an adrenergic pro-drug through sequential stereoselective action of tandem target enzymes. Biochem Biophys Res Commun 189:33-39
3. Dingman S, Snyder-Leiby T, Huber R (2002) An in vivo test of the conversion of PF-5-HTP to PF-5-HT using crude enzyme from JM109 bacteria infected with the plasmid pKKAADCII (Summary). Proc Nat Meet Am Assoc Adv Sci 168:A94
4. Hayashi H, Tsukiyama F, Ishii S, Mizuguchi H, Kagamiyama H (1999) Acid-base chemistry of the reaction of aromatic L-amino acid decarboxylase and DOPA analyzed by transient and steady-state kinetics: preferential binding of the substrate with its amino group unprotonated. Biochemistry 38:15615-15622
5. Hornykiewicz O (2002) L-DOPA: from a biologically inactive amino acid to a successful therapeutic agent. Historical review article. Amino Acids Pept Proteins 23:65-70
6. 18F]Fluoro-beta-fluoromethylene-m-tyrosine derivatives show stereo, geometrical and regio specificities as in vivo central dopaminergic probes in monkeys. Nucl Med Biol 26:365-370
7. Ingram SL, Prasad BM, Amara SG (2002) Dopamine transporter-mediated conductances increase excitability of midbrain dopamine neurons. Nat Neurosci 5:971-978
8. Ishii, S, Mizuguchi, H, Nishino, J, Hayashi, H, Kagamiyama, H (1996) Functionally important residues of aromatic L-amino acid decarboxylase probed by sequence alignment and site directed mutagenesis. J Biochem 120:369-376
9. Lee SH, Kang SS, Son H, Lee YS (1998) The region of dopamine transporter encompassing the 3rd transmembrane domain is crucial for function. Biochem Biophys Res Commun. 246:347-352
10. Maharaj-Mikiel I, Jones R, Tekverk P, Bieback A, Blaho N, Guckemus S, Estok K, Thomas R, Guo CY, Dingman S (2003) The effects of 5-hydroxytryptophan (5-HTP) and perfluoro-5-hydroxytryptophan (PF-5-HTP) co-administered with fluoxetine hydrochloride on crayfish aggression (Summary). Proc Nat Meet Am Assoc Adv Sci 169:A107
11. Sagne C, Isambert MF, Vandekerckhove J, Henry JP, Gasnier B (1997) The photoactivatable inhibitor 7-azido-8-iodoketanserin labels the N terminus of the vesicular monoamine transporter from bovine chromaffin granules. Biochemistry 36:3345-3352
12. Sambrook J, Russell D (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
13. Sitte HH, Huck S, Reither H, Boehm S, Singer EA, Pifl C (1998) Carrier-mediated release, transport rates, and charge transfer induced by amphetamine, tyramine, and dopamine in mammalian cells transfected with the human dopamine transporter. J Neurochem 71:1289-1297
14. Tanaka T, Horio Y, Taketoshi M, Imamura I, Ando-Yamamoto M, Kangawa K, Matsuo H, Kuroda M, Wada H (1989) Molecular cloning and sequencing of a cDNA of rat dopa decarboxylase: partial amino acid homologies with other enzymes synthesizing catecholamines. Proc Natl Acad Sci USA 86:8142-8146
15. 2-dopamine agonist on acetylcholine concentration in corpus striatum of rat brain. Neural Transm 58:55-67