Field studies on the environmental fate of the Cry1Ab Bt-toxin produced by transgenic maize (MON810) and its effect on bacterial communities in the maize rhizosphere

Molecular Ecology

Volumn 14, Issue 8, 2005, Pages 2539-2551

  Baumgarte, Susanne ^a   Tebbe, Christoph C ^a  

^a INSTITUTE FOR EPIDEMIOLOGY AND PATHOGEN DIAGNOSTICS   (Germany)

Author keywords

Microbial community analysis; MON810; PCR SSCP; Persistence; Rhizosphere; Soil; Transgenic maize

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; ENDOTOXIN; HEMOLYSIN; INSECTICIDAL CRYSTAL PROTEIN, BACILLUS THURINGIENSIS; RNA 16S;

ANALYSIS OF VARIANCE; ARTICLE; BACTERIUM; CLUSTER ANALYSIS; COMPARATIVE STUDY; DRUG EFFECT; ENVIRONMENT; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; GERMANY; MAIZE; METABOLISM; MICROBIOLOGY; SINGLE STRAND CONFORMATION POLYMORPHISM; SOIL; TRANSGENIC PLANT;

ANALYSIS OF VARIANCE; BACTERIA; BACTERIAL PROTEINS; BACTERIAL TOXINS; CLUSTER ANALYSIS; ENDOTOXINS; ENVIRONMENT; ENZYME-LINKED IMMUNOSORBENT ASSAY; GERMANY; HEMOLYSIN PROTEINS; PLANTS, GENETICALLY MODIFIED; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; RNA, RIBOSOMAL, 16S; SOIL; SOIL MICROBIOLOGY; ZEA MAYS;

BACILLUS THURINGIENSIS; ZEA MAYS;

EID: 21244448034     PISSN: 09621083     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-294X.2005.02592.x     Document Type: Article

References (44)

1. Bassam BJ, Caetanoanolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry, 196, 80-83.
2. Blackwood CB, Buyer JS (2004) Soil microbial communities associated with Bt and non-Bt corn in three soils. Journal of Environmental Quality, 33, 832-836.
3. Brosius J, Palmer ML, Kennedy PJ, Noller HF (1978) Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences, USA, 75, 4801-4805.
4. Conner AJ, Glare TR, Nap JP (2003) The release of genetically modified crops into the environment - Part II. Overview of ecological risk assessment. Plant Journal, 33, 19-46.
5. Crecchio C, Stotzky G (1998) Insecticidal activity and biodegradation of the toxin from Bacillus thuringiensis subsp. kurstaki bound to humic acids from soil. Soil Biology and Biochemistry, 30, 463-470.
6. Crecchio C, Stotzky G (2001) Biodegradation and insecticidal activity of the toxin from Bacillus thuringiensis subsp. kurstaki bound on complexes of montmorillonite-humic acids-A1 hydroxypolymers. Soil Biology & Biochemistry, 33, 573-581.
7. Devare MH, Jones CM, Thies JE (2004) Effect of Cry3Bb transgenic corn and tefluthrin on the soil microbial community: biomass, activity, and diversity. Journal of Environmental Quality, 33, 837-843.
8. Dohrmann AB, Tebbe CC (2004) Microbial community analysis by PCR-single-strand conformation polymorphism (PCR-SSCP). In: Molecular Microbial Ecology Manual (eds Kowalchuk GA, de Bruijn FJ, Head IM, Akkermans AD, van Elsas JD), pp. 809-838. Kluwer Academic Publishers, Dordrecht, The Netherlands.
9. Gomes NCM, Heuer H, Schönfeld J, Costa R, Mendonca-Hagler L, Smalla K (2001) Bacterial diversity of the rhizosphere of maize (Zea mays) grown in tropical soil studied by temperature gradient gel electrophoresis. Plant and Soil, 232, 167-180.
10. Gyamfi S, Pfeifer U, Stierschneider M, Sessitsch A (2002) Effects of transgenic glufosinate-tolerant oilseed rape (Brassica napus) and the associated herbicide application on eubacterial and Pseudomonas communities in the rhizosphere. Federation of European Microbiological Societies (FEMS) Microbiology Ecology, 41, 181-190.
11. Heuer H, Krsek M, Baker P, Smalla K, Wellington EMH (1997) Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Applied and Environmental Microbiology, 63, 3233-3241.
12. Heuer H, Smalla K (1999) Bacterial phyllosphere communities of Solanum tuberosum L. and T4-lysozyme-producing transgenic variants. FEMS Microbiology Ecology, 28, 357-371.
13. Hopkins DW, Gregorich EG (2003) Detection and decay of the Bt endotoxin in soil from a field trial with genetically modified maize. European Journal of Soil Science, 54, 793-800.
14. James C (2003) Global Status of Commercialized Transgenic Crops: 2003; ISAAA Briefs no. 30: Preview. ISAAA, Ithaca, New York.
15. Koskella J, Stotzky G (1997) Microbial utilization of free and claybound insecticidal toxins from Bacillus thuringiensis and their retention of insecticidal activity after incubation with microbes. Applied and Environmental Microbiology, 63, 3561-3568.
16. Koskella J, Stotzky G (2002) Larvicidal toxins from Bacillus thuringiensis subspp. kurstaki, morrisoni (strain tenebrionis), and israelensis have no microbicidal or microbiostatic activity against selected bacteria, fungi, and algae in vitro. Canadian Journal of Microbiology, 48, 262-267.
17. Laemmli (1970) Cleavage of structural proteins during assembly of head of bacteriophage-T4. Nature, 227, 680-685.
18. Meier U (2001) Growth Stages of Mono- and Dicotyledonous Plants, 2nd edn, Federal Biological Research Centre for Agriculture and Forestry, Braunschweig, Germany.
19. Mendelsohn M, Kough J, Vaituzis Z, Matthews K (2003) Are Bt crops safe? Nature Biotechnology, 21, 1003-1009.
20. Milling A, Smalla K, Maidl FX, Schloter M, Munch JC (2004) Effects of transgenic potatoes with an altered starch composition on the diversity of soil and rhizosphere bacteria and fungi. Plant and Soil, 226, 23-39.
21. Nguyen Thu H (2004) Sicherheitsforschung und Monitoringmethoden zum Anbau von Bt-Mais: Expression, Nachweis und Wirkung von rekombinantem Cry1Ab in heterologen Expressionssystemen. Dissertation, Georg-August-Universität, Göttingen.
22. Pagel-Wieder S, Gessler F, Niemeyer J, Schröder D (2004) Adsorption of the Bacillus thuringiensis toxin (Cry1Ab) on Na-montmorillonite and on the clay fractions of different soils. Journal of Plant Nutrition and Soil Science, 167, 184-188.
23. Palm CJ, Donegan K, Harris D, Seidler RJ (1994) Quantification in soil of Bacillus thuringiensis var. kurstaki delta-endotoxin from transgenic plants. Molecular Ecology, 3, 145-151.
24. Palm CJ, Schaller DL, Donegan KK, Seidler RJ (1996) Persistence in soil of transgenic plant produced Bacillus thuringiensis var. kurstaki delta-endotoxin. Canadian Journal of Microbiology, 42, 1258-1262.
25. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
26. Saxena O, Flores S, Stotzky G (1999) Insecticidal toxin in root exudates from Bt corn. Nature, 402, 480-480.
27. Saxena D, Flores S, Stotzky G (2002) Bt toxin is released in root exudates from 12 transgenic corn hybrids representing three transformation events. Soil Biology & Biochemistry, 34, 133-137.
28. Saxena D, Stewart CN, Altosaar I, Shu QY, Stotzky G (2004) Larvicidal Cry proteins from Bacillus thuringiensis are released in root exudates of transgenic B. thuringiensis corn, potato, and rice but not of B. thuringiensis canola, cotton, and tobacco. Plant Physiology and Biochemistry, 42, 383-387.
29. Saxena D, Stotzky G (2000) Insecticidal toxin from Bacillus thuringiensis is released from roots of transgenic Bt corn in vitro and in situ. FEMS Microbiology Ecology, 33, 35-39.
30. Saxena D, Stotzky G (2001) Bacillus thuringiensis (Bt) toxin released from root exudates and biomass of Bt corn has no apparent effect on earthworms, nematodes, protozoa, bacteria, and fungi in soil. Soil Biology & Biochemistry, 33, 1225-1230.
31. Schmalenberger A, Schwieger F, Tebbe CC (2001) Effect of primers hybridizing to different evolutionarily conserved regions of the small-subunit rRNA gene in PCR-based microbial community analyses and genetic profiling. Applied and Environmental Microbiology, 67, 3557-3563.
32. Schmalenberger A, Tebbe CC (2002) Bacterial community composition in the rhizosphere of a transgenic, herbicide-resistant maize (Zea mays) and comparison to its non-transgenic cultivar Bosphore. FEMS Microbiology Ecology, 40, 29-37.
33. Schmalenberger A, Tebbe CC (2003a) Bacterial diversity in maize rhizospheres: conclusions on the use of genetic profiles based on PCR-amplified partial small subunit rRNA genes in ecological studies. Molecular Ecology, 12, 251-261.
34. Schmalenberger A, Tebbe CC (2003b) Genetic profiling of non-cultivated bacteria from the rhizospheres of sugar beet (Beta vulgaris) reveal field and annual variability but no effect of a transgenic herbicide resistance. Canadian Journal of Microbiology, 49, 1-8.
35. Schwieger F, Tebbe CC (1998) A new approach to utilize PCR-single-strand conformation polymorphism for 16S rRNA gene-based microbial community analysis. Applied and Environmental Microbiology, 64, 4870-4876.
36. Sims SR, Holden LR (1996) Insect bioassay for determining soil degradation of Bacillus thuringiensis subsp. kurstaki Cry1A(b) protein in corn tissue. Environmental Entomology, 25, 659-664.
37. Smalla K, Wieland G, Buchner A et al. (2001) Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed. Applied and Environmental Microbiology, 67, 4742-4751.
38. Tapp H, Calamai L, Stotzky G (1994) Adsorption and binding of the insecticidal proteins from Bacillus thuringiensis subsp. kurstaki and subsp. tenebrionis on clay-minerals. Soil Biology and Biochemistry, 26, 663-679.
39. Tapp H, Stotzky G (1995) Insecticidal activity of the toxins from Bacillus thuringiensis subsp. kurstaki and tenebrionis adsorbed and bound on pure and soil clays. Applied and Environmental Microbiology, 61, 1786-1790.
40. Tapp H, Stotzky G (1998) Persistence of the insecticidal toxin from Bacillus thuringiensis subsp. kurstaki in soil. Soil Biology & Biochemistry, 30, 471-476.
41. Tebbe CC, Schmalenberger A, Peters S, Schwieger F (2001) Single-strand conformation polymorphism (SSCP) for microbial community analysis. In: Environmental Molecular Microbiology: Protocols and Applications (ed. Rochelle PA), pp. 161-175. Horizon Scientific Press, Wymondham, UK.
42. Venkateswerlu G, Stotzky G (1992) Binding of the protoxin and toxin proteins of Bacillus thuringiensis subsp. kurstaki on clay minerals. Current Microbiology, 25, 225-233.
43. Weisburg WG, Barns SM, Pelletier DA, Lane DG (1991) 16S ribosomal DNA amplification for phylogenetic analyses. Journal of Bacteriology, 173, 697-703.
44. Zwahlen C, Hilbeck A, Gugerli P, Nentwig W (2003) Degradation of the Cry1Ab protein within transgenic Bacillus thuringiensis corn tissue in the field. Molecular Ecology, 12, 765-775.