Serologic immunoreactivity to Neospora caninum antigens in dogs determined by indirect immunofluorescence, western blotting and dot-ELISA

Veterinary Parasitology

Volumn 130, Issue 1-2, 2005, Pages 73-79

  Pinheiro, A M ^a,b   Costa, M F ^a   Paule, B ^a   Vale, V ^a   Ribeiro, M ^a   Nascimento, I ^a   Schaer, R E ^a   Almeida, M A O ^a   Meyer, R ^a   Freire, S M ^a  

^a FEDERAL UNIVERSITY OF BAHIA   (Brazil)

^b Instituto de Ciencias da Sau   (Brazil)

Author keywords

Dogs; dot ELISA; Immunodiagnosis; Neospora caninum

Indexed keywords

PARASITE ANTIBODY; PARASITE ANTIGEN; PYROXYLIN;

AGGLUTINATION TEST; ANIMAL CELL; ANTIBODY DETECTION; ARTICLE; CONTROLLED STUDY; DOG; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACT; FEMALE; IMMUNOFLUORESCENCE; IMMUNOFLUORESCENCE TEST; IMMUNOREACTIVITY; INTERMETHOD COMPARISON; LABORATORY TEST; MALE; MEMBRANE; NEOSPORA CANINUM; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; REACTION ANALYSIS; RELIABILITY; SAMPLE; SENSITIVITY AND SPECIFICITY; SERUM; STANDARDIZATION; TOXOPLASMA GONDII; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, PROTOZOAN; BLOTTING, WESTERN; COCCIDIOSIS; CROSS REACTIONS; DOG DISEASES; DOGS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; MALE; NEOSPORA; SENSITIVITY AND SPECIFICITY; TOXOPLASMA; TOXOPLASMOSIS, ANIMAL;

ANIMALIA; BOVINAE; CANIS FAMILIARIS; COCCIDIA; NEOSPORA CANINUM; PROTOZOA; TOXOPLASMA GONDII;

EID: 21044434323     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetpar.2005.03.018     Document Type: Article

References (21)

1. A.T. Andrews Electrophoresis: Theory, Techniques and Biochemical and Clinical Applications 1986 Oxford University Press
2. C. Bjorkman, A. Lunden, J. Holmdahl, J. Barber, A.J. Trees, and A. Uggla Neospora caninum in dogs: detection of antibodies by ELISA using an ISCOM antigen Parasite Immunol. 16 1994 643 648
3. C. Bjorkman, O.J. Holmdahl, and A. Uggla An indirect enzyme-linked immunoassay (ELISA) for demonstration of antibodies to Neospora caninum in serum and milk of cattle Vet. Parasitol. 68 1997 251 260
4. C. Björkman, and A. Uggla Serological diagnosis of Neospora caninum infection Int. J. Parasitol. 29 1999 1497 1507
5. J.P. Dubey, A.I. Hattel, D.S. Lindsay, and M.J. Topper Neonatal Neospora caninum infection in dogs: isolation of the causative agent and experimental transmission J. Am. Vet. Med. Assoc. 193 1988 1259 1263
6. J.P. Dubey Recent advances in Neospora and neosporosis Vet. Parasitol. 84 1999 349 367
7. J.P. Dubey Review of Neospora caninum and neosporosis in animals Kor. J. Parasitol. 41 2003 1 16
8. L.F. Gondim, A.M. Pinheiro, P.O. Santos, E.E. Jesus, M.B. Ribeiro, H.S. Fernandes, M.A. Almeida, S.M. Freire, R. Meyer, and M.M. Mcallister Isolation of Neospora caninum from the brain of a naturally infected dog, and production of encysted bradyzoites in gerbils Vet. Parasitol. 101 2001 1 7
9. A. Hemphill, N. Fuchs, S. Sonda, and A. Hehl The antigenic composition of Neospora caninum Int. J. Parasitol. 29 1999 1175 1188
10. U.K. Laemmli Nature (Lond.) 227 1970 680
11. O. Lowry Protein measurement with the folin phenol reagent J. Biol. Chem. 193 1951 265 275
12. M.M. McAllister, J.P. Dubey, D.S. Lindsay, W.R. Jolley, R.A. Wills, and A.M. McGuire Dogs are definitive hosts of Neospora caninum Int. J. Parasitol. 28 1998 1473 1478
13. T.W. Mineo, Silva Da, G.H. Costa, A.C. Von Ancken, L.H. Kasper, M.A. Souza, D.D. Cabral, A.J. Costa, and J.R. Mineo Detection of IgG antibodies to Neospora caninum and Toxoplasma gondii in dogs examined in a veterinary hospital from Brazil Vet. Parasitol. 98 2001 239 245
14. A.R. Moen, W. Wouda, M.F. Mul, E.A. Graat, and T. Van Werven Increased risk of abortion following Neospora caninum abortion outbreaks: a retrospective and prospective cohort study in four dairy herds Theriogenology 49 1998 1301 1309
15. T. Osawa, J. Wastling, S. Maley, D. Buxton, and E.A. Innes A multiple antigen ELISA to detect Neospora-specific antibodies in bovine sera, bovine foetal fluids, ovine and caprine sera Vet. Parasitol. 79 1998 19 34
16. J. Pare, Sk Hietala, and M.C. Thurmond An enzyme-linked immunosorbent assay (ELISA) for serological diagnosis of Neospora sp. infection in cattle J. Vet. Diagn. Invest. 7 1995 352 359
17. Sampaio, I.B.M., 1998. Estatística aplicada a experimentaç ão animal. Brazil, 138 pp.
18. G. Schares, M. Rauser, P. Sondgen, P. Rehberg, A. Barwald, J.P. Dubey, R. Edelhofer, and F.J. Conraths Use of purified tachyzoite surface antigen p38 in an ELISA to diagnose bovine neosporosis Int. J. Parasitol. 30 2000 1123 1130
19. G. Schares, U. Wenzel, T. Muller, and F.J. Conraths Serological evidence for naturally occurring transmission of Neospora caninum among foxes (Vulpes vulpes) Int. J. Parasitol. 31 2001 418 423
20. S. Stenlund, C. Bjorkman, O.J. Holmdahl, H. Kindahl, and A. Uggla Characterization of a Swedish bovine isolate of Neospora caninum Parasitol Res. 83 1997 214 219
21. W. Wouda, J. Brinkhof, C. Van Maanen, A.J. De Gee, and A.R. Moen Serodiagnosis of neosporosis in individual cows and dairy herds: a comparative study of three enzyme-linked immunosorbent assays Clin. Diagn. Lab. Immunol. 5 1998 711 716