A rapid and simplified ELISA using whole blood samples of Schistosoma japonicum-infected rabbits

Southeast Asian Journal of Tropical Medicine and Public Health

Volumn 36, Issue 2, 2005, Pages 445-450

  Hirose, Yoshinori ^a   Kirinoki, Masashi ^a   Chigusa, Yuichi ^a   Matsuda, Hajime ^a  

^a DOKKYO MEDICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PARASITE ANTIGEN; PHOSPHATE BUFFERED SALINE; POLYSORBATE;

ANIMAL; ANIMAL MODEL; ARTICLE; BLOOD; BLOOD SAMPLING; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOLOGY; INCUBATION TIME; INTERMETHOD COMPARISON; MASS SCREENING; METHODOLOGY; NONHUMAN; OPTICAL DENSITY; RABBIT; SCHISTOSOMA JAPONICUM; SCHISTOSOMIASIS; TEMPERATURE; TIME;

EID: 20444431529     PISSN: 01251562     EISSN: 26975718     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Boctor FN, Stek MJ, Peter JB, Kamal R. Simplification and standardization of dot-ELISA for human schistosomiasis mansoni. J Parasitol 1987; 73: 589-92.
2. Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochem 1971; 8: 871-4.
3. Hamilton JV, Klinkert M, Doenhoff MJ. Diagnosis of schistosomiasis: antibody detection, with notes on parasitological and antigen detection methods. Parasitology 1998; 117: S41-57.
4. Hancock K, Tsang VCW. Development and optimization of the FAST-ELISA for detecting antibodies to Schistosoma mansoni. J Immunol Methods 1986; 92: 167-76.
5. Ismail MM, Bruce JI, Attia M, Tayel SE, Sabah AA, El-Ahmedawy BA. The detection of IgE by radio allergosorbent technique (R.A.S.T.) and ELISA in Egyptian cases of schistosomiasis. J Egypt Soc Parasit 1989; 19: 29-34.
6. Kamal KA, Shaheen HI, El-Said AA. Applicability of ELISA on buffer-eluates of capillary blood spotted on filter papers for the diagnosis and clinical staging of human schistosomiasis. Trop Geogr Med 1994; 46: 138-41.
7. Matsuda H, Tanaka H, Blas BL, Noseñas JS, Tokawa T, Ohsawa S. Evaluation of ELISA with ABTS, 2-2′-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med 1984; 54: 131-8.
8. Moser D, Doumbo O, Klinkert M. The humoral response to heat shock protein 70 in human and murine Schistosomiasis mansoni. Parasite Immunol 1990; 12: 341-52.
9. Tsang VCW, Wilson BC, Maddison SE. Kinetic studies of a quantitative single-tube enzyme-linked immunosorbent assay. Clin Chem 1980; 26: 1255-60.
10. Tsang VCW, Tao YX, Qui LS, Xue HC. Fractionation and quantitation of egg antigens from Schistosoma japonicum by the single-tube kinetic-dependent enzyme-linked immunosorbent assay (k-ELISA): higher antigenic activity in urea-soluble than in aqueous-soluble fractions. J Parasitol 1982; 68: 1034-43.
11. Voller A, Bidwell D, Huldt G, Engvall E. A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bull WHO 1974; 51: 209-11.
12. Weiss N, Stürchler D, Dietrich FM. Radioallergosorbent and indirect fluorescent antibody tests in immunodiagnosis of schistosomiasis. Lancet 1978; 312: 1231-3.