Identification and funtional characterization of three postsynaptic short-chain neurotoxins from hydrophiinae, Lapemis hardwickii gray

Acta Biochimica et Biophysica Sinica

Volumn 33, Issue 4, 2001, Pages 461-462

  Zhong, Xiao Fen ^a   Peng, Li Sheng ^a   Wu, Wen Yan ^a   Wei, Jian Wen ^a   Yang, Hong ^a   Yang, Yan Zhen ^a   Xu, An Long ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Biological activity; Fusion expression; Lapemis hardwickii Gray; Postsynaptic short chain neurotoxin

Indexed keywords

EID: 20444414939     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Harvey A L ed. Snake Toxin, New York; Pergamon Press, 1991, 165-222
2. Servent D, Winckler-Dietrich V, Hu H Y, Kessler P, Drevet P, Bertrand D, Ménez A. Only snake curaremimetic toxins with a fifth disulfide bond have high affinity for the neuronal α7 nicotinic receptor. J Biol Chem, 1997, 272(39): 24279-24286
3. Tamiya T, Ohno S, Nishimura E, Fujimi T J, Tsuchiya T. Complete nucleotide sequences of cDNAs encoding long chain α-neurotoxins from sea krait, Laticauda semifasciata. Toxicon, 1999, 37(1): 181-185
4. Afifiyan F, Armugam A, Gopalakrishnakone P, Tan N H, Tan C H, Jeyaseelan K. Four new postsynaptic neurotoxins from Naja naja sputatrix venom: cDNA cloning, protein expression, and phylogenetic analysis. Toxicon, 1998, 36(12): 1871-1885
5. Trémeau O, Lemaire C, Drevet P, Pinkasfeld S, Ducancel F, Boulain J C, Menez A. Genetic engineering of snake toxins. The functional site of erabutoxin a, as delineated by site-directed mutagenesis, includes variant residues. J Biol Chem, 1995, 270(16): 9362-9369
6. Pillet L, Tremeau O, Ducancel F, Drevet P, Zinn-Justin S, Pinkasfeld S, Boulain J C et al. Genetic engineering of snake toxins. Role of invariant residues in the structural and functional properties of a curaremimetic toxin, as probed by site-directed mutagenesis. J Biol Chem, 1993, 268(2): 909-916
7. Ducancel F, Merienne K, Fromen-Romano C, Tremeau O, Pillet L, Drevet P, Zinn-Justin S et al. Mimicry between receptors and antibodies. Identification of snake toxin determinants recognized by the acetylcholine receptor and an acetylcholine receptor-mimicking monoclonal antibody. J Biol Chem, 1996, 271(49): 31345-31353
8. Antil S, Servent D, Ménez A. Variability among the sites by which curaremimetic toxins bind to Torpedo acetylcholine receptor, as revealed by identification of the functinal residues of α-Cobratoxin. J Biol Chem, 1999, 274(49): 34851-34858
9. Gong N, Armugam A, Jeyaseelan K. Postsynaptic short-chain neurotoxins from Pseudonaja textilis cDNA cloning, expression and protein characterization. Eur J Biochem, 1999, 265(3): 982-989
10. Qin G P ed. Chinese poisonous snake research, 2nd ed, Guangxi: Guangxi Scientific and Technical Publishers, 1998, 36-39
11. Zhong X Y, Pan H, Wu J, Van G Z, Wu X F, Zhou Y C. Clonging and functional expression of neurotoxin cDNA from Naja naja atra. Acta Biochimica et Biophysica Sinica, 2000, 32(1): 90-93
12. 2 gene from sea snake Lapemis hardwickii Gray venom. Acta Biochimica et Biophysica Sinica, 2001, 33(3): 345-350
13. Lu S D ed. Modern Molecular Biological Technology, Beijing: High Education Press, 1993, 147-149
14. Sambrook J, Fritsch E F, Maniatis T. Molecular Clonging: A Laboratory Manual, 2nd ed, New York: Cold Spring Harbor Laboratory Press, 1989
15. Xu S Y, Bian R L, Chen X eds. Methods in pharmacology, 2nd ed, Beijing: People Hygiene Press, 1994, 699-701
16. Fox J W, Elzinga M, Tu A T. Amino acid sequence of a snake neurotoxin from the venom of Lapemis hardwickii and the detection of a sulfhydryl group by laser Raman spectroscopy. FEBS Lett, 1977, 80(1): 217-220
17. Deshimaru M, Ogawa T, Nakashima K, Nobuhisa I, Chijiwa T, Shimohigashi Y, Fukumaki Y et al. Accelerated evolution of crotalinae snake venom gland serine proteases. FEBS Lett, 1996, 397: 83-88
18. 2. J Mol Evol, 1995, 41(6): 867-877
19. Chang L S, Lin J, Chou Y C, Hong E. Genomic structures of cardiotoxin 4 and cobrotoxin from Naja naja atra (Taiwan cobra). Biochem Biophys Res Commun, 1997, 239(3): 756-762
20. 2 from snake venom to human secreted forms. J Mol Evol, 1990, 31(3): 228-238
21. Daltry J C, Wuster W, Thorpe R S. Diet and snake venom evolution. Nature, 1996, 379: 537-540
22. Servent D, Antil-Delbeke S, Gaillard C, Corringer P J, Changeux J P, Menez A. Molecular characterization of the specificity of interactions of various neurotoxins on two distinct nicotinic acetylcholine receptors. Eur J Pharmacol, 2000, 393(1-3): 197-204