Constitutive and ligand-induced internalization of EGFP-tagged galanin R2 and Rl receptors in PC12 cells

Neuropeptides

Volumn 39, Issue 3, 2005, Pages 173-178

  Xia, Sheng ^a,b   Kjaer, Svend ^a   Zheng, Kang ^a   Hu, Ping Sheng ^c   Xu, Tao ^b   Hökfelt, Tomas ^a   Xu, Zhi Qing David ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c Biovitrum AB ^*  (Sweden)

Author keywords

Clathrin dependant endocytic pathway; Constitutive internalization; Neuropeptide; Receptor trafficking; Transfection

Indexed keywords

AR M1896; CLATHRIN; ENHANCED GREEN FLUORESCENT PROTEIN; GALANIN; GALANIN RECEPTOR 1; GALANIN RECEPTOR 2; LIGAND; NEUROPEPTIDE;

ANIMAL CELL; CELL MEMBRANE; CELL STRAIN; CELL TRANSPORT; CONFERENCE PAPER; CONTROLLED STUDY; DOSE RESPONSE; GENETIC TRANSFECTION; INCUBATION TIME; NONHUMAN; PHEOCHROMOCYTOMA CELL; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; RAT;

ANIMALS; BRADYKININ; CALCIUM SIGNALING; ENDOCYTOSIS; FLUORESCENT DYES; GALANIN; GREEN FLUORESCENT PROTEINS; LIGANDS; NEURONS; PC12 CELLS; PEPTIDE FRAGMENTS; PROTEIN TRANSPORT; RATS; RECEPTOR, GALANIN, TYPE 1; RECEPTOR, GALANIN, TYPE 2; TRANSFECTION; XANTHENES;

EID: 20444411177     PISSN: 01434179     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.npep.2005.02.001     Document Type: Conference Paper

References (27)

1. C. Baratti-Elbaz, N. Ghinea, O. Lahuna, H. Loosfelt, C. Pichon, and E. Milgrom Internalization and recycling pathways of the thyrotropin receptor Mol. Endocrinol. 13 1999 1751 1765
2. T. Bartfai, G. Fisone, and U. Langel Galanin and galanin antagonists: molecular and biochemical perspectives Trends Pharmacol. Sci. 13 1992 312 317
3. R. Bartz, C. Benzing, and O. Ullrich Reconstitution of vesicular transport to Rab11-positive recycling endosomes in vitro Biochem. Biophys. Res. Commun. 312 2003 663 669
4. T. Branchek, K.E. Smith, and M.W. Walker Molecular biology and pharmacology of galanin receptors Ann. NY Acad. Sci. 863 1998 94 107
5. T.A. Branchek, K.E. Smith, C. Gerald, and M.W. Walker Galanin receptor subtypes TiPS 21 2000 109 116
6. T. Costa, and A. Herz Antagonists with negative intrinsic activity at delta opioid receptors coupled to GTP-binding proteins Proc. Natl. Acad. Sci. USA 86 1989 7321 7325
7. G. Daukas, and S.H. Zigmond Inhibition of receptor-mediated but not fluid-phase endocytosis in polymorphonuclear leukocytes J. Cell Biol. 101 1985 1673 1679
8. A. Durrbach, D. Louvard, and E. Coudrier Actin filaments facilitate two steps of endocytosis J. Cell Sci. 109 Pt 2 1996 457 465
9. Z. Fathi, P.M. Battaglino, L.G. Iben, H. Li, E. Baker, D. Zhang, R. McGovera, C.D. Mahle, G.R. Sutherland, T.P. lismaa, K.E. Dickinson, and I.A. Zimanyi Molecular characterization, pharmacological properties and chromosomal localization of the human GALR2 galanin receptor Mol. Brain Res. 58 1998 156 169
10. E. Habert-Ortoli, B. Amiranoff, I. Loquet, M. Laburthe, and J.F. Mayaux Molecular cloning of a functional human galanin receptor Proc. Natl. Acad. Sci. USA 91 1994 9780 9783
11. J.E. Heuser, and R.G. Anderson Hypertonic media inhibit receptor-mediated endocytosis by blocking clathrin-coated pit formation J. Cell Biol. 108 1989 389 400
12. T.P. Iismaa, and J. Shine Galanin and galanin receptors Results Probl. Cell Differ. 26 1999 257 291
13. H.X.R. Liu, R. Schmidt, W. Brown, K. Payza, L. Hodzic, C. Pou, C. Godbout, and T. Hökfelt Receptor mechanisms underlying the bi-directional role of galanin in pain processing in the spinal cord Proc. Natl. Acad. Sci. USA 98 2001 9960 9964
14. T. Melander, C. Köhler, S. Nilsson, T. Hökfelt, E. Brodin, E. Theodorsson, and T. Bartfai Autoradiographic quantification and anatomical mapping of 1251-Galanin binding in the rat central nervous system J. Chem. Neuroanat. 1 1988 213 233
15. F. Mennicken, C. Hoffert, M. Pelletier, S. Ahmad, and D. O'Donnell Restricted distribution of galanin receptor 3 (GalR3) mRNA in the adult rat central nervous system J. Chem. Neuroanat. 24 2002 257 268
16. S. Miserey-Lenkei, C. Parnot, S. Bardin, P. Corvol, and E. Clauser Constitutive internalization of constitutively active agiotensin II AT(1A) receptor mutants is blocked by inverse agonists J. Biol. Chem. 277 2002 5891 5901
17. D. O'Donnell, S. Ahmad, C. Wahlestedt, and P. Walker Expression of the novel galanin receptor subtype GALR2 in the adult rat CNS: distinct distribution from GALR1 J. Comp. Neurol. 409 1999 469 481
18. C. Parnot, S. Miserey-Lenkei, S. Bardin, P. Corvol, and E. Clauser Lessons from constitutively active mutants of G protein-coupled receptors Trends Endocrinol. Metab. 13 2002 336 343
19. 125I-galanin binding sites in the rat central nervous system Peptides 7 1986 1029 1042
20. K.E. Smith, C. Forray, M.W. Walker, K.A. Jones, J.A. Tamm, J. Bard, T.A. Branchek, D.L. Linemeyer, and C. Gerald Expression cloning of a rat hypothalamic galanin receptor coupled to phosphoinositide turnover J. Biol. Chem. 272 1997 24612 24616
21. A. Subtil, I. Gaidarov, K. Kobylarz, M.A. Lampson, J.H. Keen, and T.E. McGraw Acute cholesterol depletion inhibits clathrin-coated pit budding Proc. Natl. Acad. Sci. USA 96 1999 6775 6780
22. K. Tatemoto, A. Rökaeus, H. Jörnvall, T.J. McDonald, and V. Mutt Galanin - a novel biologically active peptide from porcine intestine FEBS Lett. 164 1983 124 128
23. M. von Zastrow, and B.K. Kobilka Ligand-regulated internalization and recycling of human beta 2-adrenergic receptors between the plasma membrane and endosomes containing transferrin receptors J. Biol. Chem. 267 1992 3530 3538
24. S. Wang, A. Clemmons, C. Strader, and M. Bayne Evidence for hydrophobic interaction between galanin and the GalR1 galanin receptor and GalR1-mediated ligand internalization: fluorescent probing with a fluorescein-galanin Biochemistry 37 1998 9528 9535
25. S.M. Waters, and J.E. Krause Distribution of galanin-1, -2 and -3 receptor messenger RNAs in central and peripheral rat tissues Neuroscience 95 2000 265 271
26. J.W. Woods, J. Goodhouse, and M.G. Farquhar Transferrin receptors and cation-independent mannose-6-phosphate receptors deliver their ligands to two distinct subpopulations of multivesicular endosomes Eur. J. Cell Biol. 50 1989 132 143
27. S. Xia, S. Kjaer, K. Zheng, P.S. Hu, L. Bai, J.Y. Jia, R. Rigler, A. Pramanik, T. Xu, T. Hökfelt, and Z.Q. Xu Visualization of a functionally enhanced GFP-tagged galanin R2 receptor in PC12 cells: constitutive and ligand-induced internalization Proc. Natl. Acad. Sci. USA 101 2004 15207 15212