Expression and characterization of the carboxyl esterase Rv3487c from Mycobacterium tuberculosis

Protein Expression and Purification

Volumn 42, Issue 1, 2005, Pages 59-66

  Zhang, Min ^a   Wang, Jia Dong ^a   Li, Zuo Feng ^b   Xie, Jun ^a   Yang, Yan Ping ^a   Zhong, Yang ^b   Wang, Hong Hai ^a  

^a FUDAN UNIVERSITY   (China)

^b FUDAN UNIVERSITY   (China)

Author keywords

Catalytic triad; Esterase; LipF; Mycobacterium tuberculosis; Rv3487c

Indexed keywords

CARBOXYLESTERASE; DETERGENT; ENTEROPEPTIDASE; GLUTAMIC ACID; HISTIDINE; HYBRID PROTEIN; SERINE; THIOREDOXIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; DRUG EFFECT; ENZYME ACTIVE SITE; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MYCOBACTERIUM TUBERCULOSIS; PH; PROTEIN TERTIARY STRUCTURE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; TEMPERATURE;

AMINO ACID SEQUENCE; CARBOXYLESTERASE; CATALYTIC DOMAIN; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DETERGENTS; ENTEROPEPTIDASE; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GLUTAMIC ACID; HISTIDINE; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MYCOBACTERIUM TUBERCULOSIS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; TEMPERATURE; THIOREDOXIN;

ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS;

EID: 20444381377     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.03.022     Document Type: Article

References (17)

1. C. Dye, S. Scheele, P. Dolin, V. Pathania, and M.C. Raviglione Consensus statement. Global burden of tuberculosis: estimatedincidence, prevalence, and mortality by country. WHO Global Surveillance and Monitoring Project J. Am. Med. Assoc. 282 1999 677 686
2. T.C. Zahrt Molecular mechanisms regulating persistent Mycobacterium tuberculosis infection Microbes Infect. 5 2003 159 167
3. B. Saviola, S.C. Woolwine, and W.R. Bishai Isolation of acid-inducible genes of Aobacterium tuberculosis with the use of recombinase-based in vivo expression technology Infect. Immun. 71 2003 1379 1388
4. A.M. Dannenberg Immunopathogenesis of pulmonary tuberculosis Hosp. Pract. 28 1993 51 58
5. L.R. Camacho, D. Ensergueix, E. Perez, B. Gicquel, and C. Guilhot Identification of a virulence gene cluster of Mycobacterium tuberculosis by signature-tagged transposon mutagenesis Mol. Microbiol. 34 1999 257 267
6. S. Canaan, D. Maurin, H. Chahinian, B. Pouilly, C. Durousseau, F. Frassinetti, L.S. Calvo, C. Cambillau, and Y. Bourne Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis Eur. J. Biochem. 271 2004 3953 3961
7. A. Camilli, and J.J. Mekalanos Use of recombinase gene fusions to identify Vibrio cholerae genes induced during infection Mol. Microbiol. 18 1995 971 983
8. D.C. Straus, M.K. Lonon, and J.C. Hutson Inhibition of rat alveolar macrophage phagocytic function by a Pseudomonas cepacia lipase J. Med. Microbiol. 37 1992 335 340
9. B. Konig, K.E. Jaeger, A.E. Sage, M.L. Vasil, and W. Konig Role of Pseudomonas aeruginosa lipase in inflammatory mediator release from human inflammatory effector cells (platelets, granulocytes, and monocytes) Infect. Immun. 64 1996 3252 3258
10. J.A. Mangan, K.M. Sole, D.A. Mitchison, and P.D. Butcher An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria Nucleic Acids Res. 25 1997 675 676
11. J. Timm, F.A. Post, L.G. Bekker, G.B. Walther, H.C. Wainwright, R. Manganelli, W.T. Chan, L. Tsenova, B. Gold, I. Smith, G. Kaplan, and J.D. McKinney Differential expression of iron-, carbon-, and oxygen-responsive mycobacterial genes in the lungs of chronically infected mice and tuberculosis patients Proc. Natl. Acad. Sci. USA 100 2003 14321 14326
12. R. Manganelli, E. Dubnau, S. Tyagi, F.R. Kramer, and I. Smith Differential expression of 10 sigma factor genes in Mycobacterium tuberculosis Mol. Microbiol. 31 1999 715 724
13. G. De Simone, V. Menchise, G. Manco, L. Mandrich, N. Sorrentino, D. Lang, M. Rossi, and C. Pedone The crystal structure of a hyper-thermophilic carboxylesterase from the archaeon Archaeoglobus fulgidus J. Mol. Biol. 314 2001 507 518
14. G. De Simone, V. Menchise, V. Alterio, L. Mandrich, M. Rossi, G. Manco, and C. Pedone The crystal structure of an EST2 mutant unveils structural insights on the H group of the carboxylesterase/lipase family J. Mol. Biol. 343 2004 137 146
15. R.A. Laskowski, M.W. MacArthur, D.S. Moss, and J.M. Thornton PROCHECK: a program to check the stereochemical quality of protein structures J. Appl. Crystallogr. 26 1993 283 291
16. S.N. Ho, H.D. Hunt, R.M. Horton, J.K. Pullen, and L.R. Pease Site-directed mutagenesis by overlap extension using the polymerase chain reaction Gene 77 1989 51 59
17. A.C. Wallace, R.A. Laskowski, and J.M. Thornton Derivation of 3D coordinate templates for searching structural databases: application to Ser-His-Asp catalytic triads in the serine proteinases and lipases Protein Sci. 5 1996 1001 1003