Molecular characterization of two novel splice variants of G alphai2 in the rat vestibular periphery

Molecular Brain Research

Volumn 137, Issue 1-2, 2005, Pages 89-97

  Cioffi, Joseph A ^a   Wackym, P Ashley ^a   Erbe, Christy B ^a   Gaggl, Wolfgang ^a   Popper, Paul ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

Author keywords

Alternative splicing; Computer modeling; Efferent vestibular system; G protein; Pseudogene; Receptor; Vestibular

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT;

ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; ENZYME BINDING; EXON; FEMALE; GENE DELETION; GENE EXPRESSION; GENE IDENTIFICATION; IN SITU HYBRIDIZATION; MALE; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSEUDOGENE; RAT; RNA SPLICING; VESTIBULAR SYSTEM;

ALTERNATIVE SPLICING; ANIMALS; BINDING SITES; EXONS; FEMALE; GTP-BINDING PROTEIN ALPHA SUBUNIT, GI2; GTP-BINDING PROTEIN ALPHA SUBUNITS, GI-GO; HAIR CELLS, VESTIBULAR; MALE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NEURONS, AFFERENT; PROTEIN ISOFORMS; PROTO-ONCOGENE PROTEINS; RATS; RNA, MESSENGER; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID; VESTIBULAR NERVE; VESTIBULE;

EID: 20444375101     PISSN: 0169328X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbrainres.2005.02.024     Document Type: Article

References (25)

1. L.C. Barritt, B. Fritzsch, and K.W. Beisel Characterization of G-protein betagamma expression in inner ear Brain Res. Mol. Brain Res. 68 1999 42 54
2. D.L. Black Mechanisms of alternative pre-messenger RNA splicing Annu. Rev. Biochem. 72 2003 291 336
3. J. Blahos, T. Fischer, I. Brabet, D. Stauffer, G. Rovelli, J. Bockaert, and J.P. Pin A novel site on the Galpha-protein that recognizes heptahelical receptors J. Biol. Chem. 276 2001 3262 3269
4. D.E. Clapham, and E.J. Neer New roles for G-protein beta gamma-dimers in transmembrane signalling Nature 365 1993 403 406
5. R. Cristobal, P.A. Wackym, J.A. Cioffi, C.B. Erbe, J.P. Roche, and P. Popper Assessment of differential gene expression in vestibular epithelial cell types using microarray analysis Mol. Brain Res. 133/1 2005 19 36
6. W.L. DeLano The PyMOL molecular graphics system 2002 DeLano Scientific San Carlos, CA, USA http://www.pymol.org
7. B.M. Denker, P.M. Boutin, and E.J. Neer Interactions between the amino- and carboxyl-terminal regions of G alpha subunits: analysis of mutated G alpha o/G alpha i2 chimeras Biochemistry 34 1995 5544 5553
8. R.A. Eatock Adaptation in hair cells Annu. Rev. Neurosci. 23 2000 285 314
9. K. Fukuda, T. Kubo, A. Maeda, I. Akiba, H. Bujo, J. Nakai, M. Mishina, H. Higashida, E. Neher, and A. Marty Selective effector coupling of muscarinic acetylcholine receptor subtypes Trends Pharmacol. Sci., Suppl. 1989 4 10
10. J.M. Goldberg, A.M. Brichta, and P.A. Wackym Efferent vestibular system: anatomy, physiology and neurochemistry A. Beitz J. Anderson Neurochemistry of the Vestibular System 2000 CRC Press Boca Raton, FL 95 135
11. J. Howard, W.M. Roberts, and A.J. Hudspeth Mechanoelectrical transduction by hair cells Annu. Rev. Biophys. Biophys. Chem. 17 1988 99 124
12. J.M. Johnson, J. Castle, P. Garrett-Engele, Z. Kan, P.M. Loerch, C.D. Armour, R. Santos, E.E. Schadt, R. Stoughton, and D.D. Shoemaker Genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays Science 302 2003 2141 2144
13. D.T. Jones, and R.R. Reed Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium J. Biol. Chem. 262 1987 14241 14249
14. M. Krawczak, J. Reiss, and D.N. Cooper The mutational spectrum of single base-pair substitutions in mRNA splice junctions of human genes: causes and consequences Hum. Genet. 90 1992 41 54
15. J.P. Montmayeur, and E. Borrelli Targeting of G alpha i2 to the Golgi by alternative spliced carboxyl-terminal region Science 263 1994 95 98
16. J.E. Slessareva, and S.G. Graber Reconstitution reveals additional roles for N- and C-terminal domains of g(alpha) in muscarinic receptor coupling Biochemistry 42 2003 7552 7560
17. M.J. Smith, and R. Iyengar Mammalian adenylyl cyclases Adv. Second Messenger Phosphoprotein Res. 32 1998 1 21
18. V. Srinivasan, M.D. Waterfield, and T.L. Blundell Comparative analysis of the regions binding beta gamma-subunits in Galpha and PH domains Biochem. Biophys. Res. Commun. 220 1996 697 702
19. T.A. Thanaraj, S. Stamm, F. Clark, J.J. Riethoven, V. Le Texier, and J. Muilu ASD: the alternative splicing database Nucleic Acids Res. 32 2004 D64 D69 (Database issue)
20. J. Valat, E. Scarfone, C. Travo, V. Homburger, and A. Sans Immunocytochemical localization of the GTP-binding protein G0 alpha in the vestibular epithelium and ganglion of the guinea-pig J. Neurocytol. 24 1995 916 924
21. P.A. Wackym, Hearing and Balance Gene Expression Database, URL: http://www.genexpression.info, Accessed January 20, 2005.
22. P.A. Wackym, C.T. Chen, A. Ishiyama, R.M. Pettis, I.A. Lopez, and L. Hoffman Muscarinic acetylcholine receptor subtype mRNAs in the human and rat vestibular periphery Cell Biol. Int. 20 1996 187 192
23. P.A. Wackym, M. Troyanovskaya, and P. Popper Partial cDNAs encoding G-protein alpha subunits in the rat vestibular periphery Neurosci. Lett. 280 2000 159 162
24. P.A. Wackym, J.A. Cioffi, C.B. Erbe, and P. Popper G-protein Golfalpha (GNAL) is expressed in the vestibular end organs and primary afferent neurons of Rattus norvegicus J. Vestib. Res. 15/1 2005 11 15
25. P.B. Wedegaertner Characterization of subcellular localization and stability of a splice variant of G alpha i2 BMC Cell Biol. 3 2002 12