Proteome analysis of brown spider venom: Identification of loxnecrogin isoforms in Loxosceles gaucho venom

Proteomics

Volumn 5, Issue 8, 2005, Pages 2167-2176

  Machado, Leandro F ^a   Laugesen, Sabrina ^b   Botelho, Elvio D ^a   Ricart, Carlos A O ^a   Fontes, Wagner ^a   Barbaro, Katia C ^c   Roepstorff, Peter ^b   Valle De Sousa, Marcelo ^a  

^a UNIVERSITY OF BRASILIA   (Brazil)

^b UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^c INSTITUTO BUTANTAN   (Brazil)

Author keywords

Brown spider; Dermonecrotic toxin; Loxnecrogin isoforms; Loxosceles gaucho; Mass spectrometry; Sphingomyelinase D; Two dimensional gel electrophoresis; Venom proteome

Indexed keywords

ISOPROTEIN; LOXNECROGIN; PROTEOME; SPHINGOMYELIN PHOSPHODIESTERASE; SPHINGOMYELINASE D; SPIDER VENOM; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BRAZIL; CAPILLARY LIQUID CHROMATOGRAPHY; EDMAN CHEMICAL SEQUENCING; ELECTROSPRAY IONIZATION QUADRUPOLE TIME OF FLIGHT TANDEM MASS SPECTROMETRY; ELECTROSPRAY MASS SPECTROMETRY; ENVENOMATION; FRACTIONATION; GEL FILTRATION; IMMUNOTHERAPY; LIQUID CHROMATOGRAPHY; LOXOSCELES GAUCHO; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; PATHOPHYSIOLOGY; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEOMICS; SEQUENCE ANALYSIS; SKIN NECROSIS; SPIDER; TWO DIMENSIONAL GEL ELECTROPHORESIS;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, LIQUID; DERMOTOXINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FILTRATION; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PHOSPHORIC DIESTER HYDROLASES; PROTEIN ISOFORMS; PROTEOME; SEQUENCE ANALYSIS, PROTEIN; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPIDER VENOMS; SPIDERS;

ARANEAE; LOXOSCELES GAUCHO; LOXOSCELES LAETA;

EID: 20044394774     PISSN: 16159853     EISSN: None     Source Type: Journal    
DOI: 10.1002/pmic.200401096     Document Type: Article

References (33)

1. Binford, G. J., Wells, M. A., Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 2003, 135, 25-33.
2. Futrell, J. M., Am. J. Med. Sci. 1992, 304, 261-267.
3. Malaque, C. M. S., Valencia, J. E. C., Cardoso, J. L. C., Franca, F. O. S. et al., Rev. Inst. Med. Trop. S. Paulo 2002, 44, 139-143.
4. Mota, I., Barbara, K. C., J. Toxinol. - Toxin Rev. 1995, 14, 401-422.
5. Sezerino, U. M., Zannin, M., Coelho, L. K., Goncalves Jr. et al., Trans. R. Soc. Trop. Med. Hyg. 1998, 92, 546-548.
6. Oliveira, K. C., Goncalves de Andrade, R. M., Giusti, A. L., Dias da Silva, W., Tambourgi, D. V., Toxicon. 1999, 37, 217-221.
7. Andrade, R. M. G., De Oliveira, K. C., Giusti, A. L., Dias da Silva, W., Tambourgi, D. V., Toxicon. 1999, 37, 627-632.
8. Bernstein, B., Ehrlich, F., J. Emerg. Med. 1986, 4, 457-462.
9. Rash, L. D., Hodgson, W. C., Toxicon. 2002, 40, 225-254.
10. Feitosa, L., Gremski, W., Veiga, S. S., Elias, M. C. et al., Toxicon. 1998, 36, 1039-1051.
11. Veiga, S. S., Feitosa, L., dos Santos, V. L., de Souza, G. A. et al., Histochem. J. 2000, 32, 397-408.
12. Veiga, S. S., da Silveira, R. B., Dreyfus, J. L., Haoach, J. et al., Toxicon. 2000, 38, 825-839.
13. Veiga, S. S., Zanetti, V. C., Braz, A., Mangili, O. C., Gremski, W., Braz. J. Med. Biol. Res. 2001, 34, 843-850.
14. Forrester, L. J., Barrett, J. T., Campbell, B. J., Arch. Biochem. Biophys. 1978, 187, 355-365.
15. Barbaro, K. C., Ferreira, M. L., Cardoso, D. F., Eickstedt, V. R., Mota, I., Braz. J. Med. Biol. Res. 1996b, 29, 1491-1497.
16. Gomez, H. F., Miller, M. J., Waggener, M. W., Lankford, H. A., Warren, J. S., Toxicon. 2001, 39, 817-824.
17. Young, A. R., Pincus, S. J., Toxicon. 2001, 39, 391-400.
18. Barbaro, K. C., Sousa, M. V., Morhy, L., Eickstedt, V. R., Mota, I., J. Protein. Chem. 1996, 15, 337-343.
19. Kurpiewski, G., Forrester, L. J., Barrett, J. T., Campbell, B. J., Biochim. Biophys. Acta 1981, 678, 467-476.
20. Tambourgi, D. V., Magnoli, F. C., van den Berg, C. W., Morgan, B. P. et al., Biochem. Biophys. Res. Commun. 1998, 257, 366-373.
21. Fernandes Pedrosa, M. F., Junqueira de Azevedo, I. L. M., Goncalves-de-Andrade, R. M., van den Berg, C. W. et al., Biochem. Biophys. Res. Commun. 2002, 298, 638-645.
22. Cunha, R. B., Barbara, K. C., Muramatsu, D., Portaro, F. C. et al., J. Protein Chem. 2003, 22, 135-146.
23. Barbara, K. C., Cardoso, J. L., Eickstedt, V. R., Mota, I., Toxicon. 1992, 30, 331-338.
24. Towbin, H., Staehelin, T., Gordon, J., Proc. Natl. Acad. Sci. USA 1979, 76, 4350-4354.
25. Guilherme, P., Fernandes, I., Barbaro, K. C., Toxicon. 2001, 39, 1333-1342.
26. Shevcnenko, A., Wilm, M., Vorm, O., Jensen, O. N. et al., Biochem. Soc. Trans. 1996, 24, 893-896.
27. Gobom, J., Nordhoff, E., Mirgorodskaya, E., Ekman, R., Roepstorff, P., J. Mass Spectrom. 1999, 34, 105-116.
28. Fontes, W., Cunha, R. B., Sousa, M. V., Morhy, L., Anal. Biochem. 1998, 256, 259-267.
29. Luciano, M. N., da Silva, P. H., Chaim, O. M., dos Santos, V. L. et al., J. Histochem. Cytochem. 2004, 52, 455-467.
30. Kalapothakis, E., Araujo, S. C., de Castro, C. S., Mendes, T. M. et al., Toxicon. 2002, 40, 1691-1699.
31. Tambourgi, D. V., de F. Fernandes Pedrosa, M., van den Berg, C. W., Goncalves-de-Andrade, R. M. et al., Mol. Immunol. 2004, 41, 831-840.
32. Thompson, J. D., Higgins, D. G., Gibson, T. J., Nucleic Acids Res. 1994, 22, 4673-4680.
33. Cisar, R. C., Fox, J. W., Geres, C. R., Toxicon. 1989, 27, 37-38.