Expression, purification, and enzymatic characterization of the dual specificity mitogen-activated protein kinase phosphatase, MKP-4

Bioorganic Chemistry

Volumn 33, Issue 1, 2005, Pages 34-44

  Hong, Suk Bong ^a   Lubben, Thomas H ^b   Dolliver, Christine M ^a   Petrolonis, Anthony J ^a   Roy, Rebecca A ^a   Li, Zhi ^a   Parsons, Thomas F ^a   Li, Ping ^a   Xu, Haiyan ^a   Reilly, Regina M ^b   Trevillyan, James M ^b   Nichols, Andrew J ^a   Tummino, Peter J ^a   Gant, Thomas G ^a  

^a MILLENNIUM PHARMACEUTICALS INC   (United States)

^b ABBOTT LABORATORIES   (United States)

Author keywords

Diabetes mellitus; Dual specificity phosphatase; Insulin signaling; MAP kinase; Protein tyrosine phosphatase superfamily; Type 2 diabetes

Indexed keywords

3 METHOXYFLUORESCEIN PHOSPHATE; 4 NITROPHENYL PHOSPHATE; 6,8 DIFLUORO 4 METHYLUMBELLIFERYL PHOSPHATE; FLUORESCEIN DERIVATIVE; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE P38; MITOGEN ACTIVATED PROTEIN KINASE PHOSPHATASE 4; PEROXOVANATE; PHOSPHORUS DERIVATIVE; PROTEIN TYROSINE PHOSPHATASE; UNCLASSIFIED DRUG;

ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; GEL FILTRATION CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SPECTROPHOTOMETRY;

CELLS, CULTURED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION; HUMANS; HYDROGEN-ION CONCENTRATION; INHIBITORY CONCENTRATION 50; INSULIN; KINETICS; MITOGEN-ACTIVATED PROTEIN KINASE 3; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN-TYROSINE-PHOSPHATASE; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY;

UNIDENTIFIED BACULOVIRUS;

EID: 19944432009     PISSN: 00452068     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bioorg.2004.08.001     Document Type: Article

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