Monoclonal versus polyclonal ELISA for assessment of fecal elastase concentration: Pitfalls of a new assay

Clinical Chemistry

Volumn 51, Issue 6, 2005, Pages 1052-1054

  Schneider, Arne ^a,b   Funk, Benjamin ^a   Caspary, Wolfgang ^a   Stein, Juergen ^a  

^a UNIVERSITY HOSPITAL FRANKFURT   (Germany)

^b GOETHE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ELASTASE; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY;

ACCURACY; ANTIGEN SPECIFICITY; ARTICLE; CHRONIC PANCREATITIS; CONTROLLED STUDY; CYSTIC FIBROSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME REPLACEMENT; FECES ANALYSIS; HUMAN; IMMUNOASSAY; INTERMETHOD COMPARISON; PANCREAS EXOCRINE INSUFFICIENCY; PROTEIN BINDING;

ANTIBODIES; ANTIBODIES, MONOCLONAL; CHRONIC DISEASE; CYSTIC FIBROSIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EXOCRINE PANCREATIC INSUFFICIENCY; FECES; HUMANS; PANCREAS, EXOCRINE; PANCREATIC ELASTASE; PANCREATITIS; REFERENCE VALUES;

EID: 19644375594     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2004.046888     Document Type: Article

References (11)

1. Loeser C, Moellgaard A. Foelsch UR. Fecal elastase-1: a novel, highly sensitive and specific tubeless pancreatic function test. Gut 1996;39: 580-6.
2. Stein J, Spichez Z, Lembcke B, Caspary WF. Evaluation of fecal elastase as a new non-invasive test for exocrine pancreatic insufficiency. Z Gastroenterol 1997;35(Suppl 1):122-9.
3. Siegmund E, Loehr JM, Schuff-Werner P. The diagnostic validity of noninvasive pancreatic function tests - a meta-analysis. Z Gastroenterol 2004; 42:1117-28.
4. Luth S, Teyssen S, Forssmann K, Kolbel C, Krummenauer F, Singer MV. Fecal elastase-1 determination: 'gold standard' of indirect pancreatic function tests? Scand J Gastroenterol 2001;36:1092-9.
5. Dominguez-Munoz JE, Hieronymus C, Sauerbruch T, Malfertheiner P. Fecal elastase test: evaluation of a new noninvasive pancreatic function test. Am J Gastroenterol 1995;90:1834-7.
6. Keim V, Teich N, Moessner J. Clinical value of a new fecal elastase test for detection of chronic pancreatitis. Clin Lab 2003;49:209-15.
7. Hardt PD, Hauenschild A. Nalop J, Marzeion AM, Porsch-Ozcurumez M, Luley C, et al. The commercially available ELISA for pancreatic elastase 1 based on polyclonal antibodies does measure an as yet unknown antigen different from purified elastase 1. Binding studies and clinical use in patients with exocrine pancreatic insufficiency. Z Gastroenterol 2003;41:903-6.
8. Lankisch PG. Now that fecal elastase is available in the United States, should clinicians start using it? Curr Gastroenterol Rep 2004;6:126-31.
9. Stein J, Jung M. Sziegoleit A, Zeuzem S, Caspary WF, Lembcke B. Immuno-reactive elastase I: clinical evaluation of a new noninvasive test of pancreatic function. Clin Chem 1996;42:222-6.
10. Waters DL, Dorney SF, Gaskin KJ, Gruca MA, O'Halloran M, Wilcken B. Pancreatic function in infants identified as having cystic fibrosis in a neonatal screening program. N Engl J Med 1990;322:303-8.
11. Bronstein MN, Sokol RJ, Abman SH, Chatfield BA, Hammond KB, Hambidge KM, et al. Pancreatic insufficiency, growth and nutrition in infants identified by newborn screening as having cystic fibrosis. J Pediatr 1992;120:533-40.