The commercially available ELISA for pancreatic elastase 1 based on polyclonal antibodies does measure an as yet unknown antigen different from purified elastase 1. Binding studies and clinical use in patients with exocrine pancreatic insufficiency

Zeitschrift fur Gastroenterologie

Volumn 41, Issue 9, 2003, Pages 903-906

  Hardt, P D ^a   Hauenschild, A ^a   Nalop, J ^a   Marzeion, A M ^a   Porsch Ozcurumez M ^a   Luley, C ^b   Sziegoleit, A ^c   Kloer, H U ^a  

^a UNIVERSITY HOSPITAL GIESSEN   (Germany)

^b OTTO VON GUERICKE UNIVERSITY   (Germany)

^c JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Chronic pancreatitis; Elastase 1; ELISA; Exocrine pancreatic function; Steatorrhea

Indexed keywords

ELASTASE; ELASTASE I; MONOCLONAL ANTIBODY; UNCLASSIFIED DRUG;

ARTICLE; CHRONIC PANCREATITIS; CORRELATION ANALYSIS; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; FECES ANALYSIS; HUMAN; MAJOR CLINICAL STUDY; PANCREAS INSUFFICIENCY; STEATORRHEA;

CHRONIC DISEASE; DATA INTERPRETATION, STATISTICAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; EXOCRINE PANCREATIC INSUFFICIENCY; FECES; HUMANS; PANCREATIC ELASTASE; PANCREATIC FUNCTION TESTS; PANCREATITIS; STEATORRHEA;

EID: 0141745884     PISSN: 00442771     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2003-41832     Document Type: Article

References (24)

1. Sziegoleit A. Purification and characterization of a cholesterol-binding protein from the human pancreas. Biochem J 1982; 207: 573-582
2. Sziegoleit A. A novel proteinase from human pancreas. Biochem J 1984; 219: 735-742
3. Sziegoleit A, Knaepler H, Peters B. ELISA for human pancreatic elastase 1. Clin Biochem 1989; 22: 79-83
4. Löser C, Mölgaard A, Fölsch UR. Faecal elastase 1: a novel, highly sensitive, and specific pancreatic function test. Gut 1996; 39: 580-586
5. Stein J, Jung M, Sziegoleit A et al. Fecal immunoreactive elastase-1: Evaluation of a new tubeless pancreatic function test in comparison with other indirect and direct tests for exocrine pancreatic function. Clin Chem 1996; 42: 222-226
6. Gullo L, Ventrucci M, Tomassetti P et al. Fecal elastase 1 determination in chronic pancreatitis. Dig Dis Sci 1999; 44: 210-213
7. Dominguez-Muñoz JE, Hieronymus C, Sauerbruch T et al. Fecal Elastase Test: Evaluation of a new noninvasive pancreatic function test. Am J Gastroenterol 1995; 90: 1834-1837
8. Lankisch PG, Schmidt I, König H et al. Faecal elastase 1: not helpful in diagnosing chronic pancreatitis associated with mild to moderate exocrine pancreatic insufficiency. Gut 1998; 42: 551-554
9. Hardt PD, Marzeion AM, Schnell-Kretschmer H. Fecal elastase 1 measurement compared with endoscopic retrograde cholangiopancreatography for the diagnosis of chronic pancreatitis. Pancreas 2002; 25: e6-9
10. Carroccio A, Verghi F, Santini B et al. Diagnostic accuracy of fecal elastase 1 assay in patients with pancreatic maldigestion or intestinal malabsorption: a collaborative study of the Italien Society of Pediatric Gastroenterology and Hepatology. Dig Dis Sci 2001; 46: 1335-1342
11. Walkowiak J, Herzig KH. Fecal elastase-1 is decreased in villous atrophy regardless of the underlying disease. Eur J Clin Invest 2001; 31: 425-430
12. Rathmann W, Haastert B, Icks A et al. Low fecal elastase 1 concentrations in Type 2 diabetes. Scand J Gastroenterol 2001; 36: 1056-1061
13. Icks A, Haastert B, Giani G et al. Low fecal elastase 1 in type 1 diabetes mellitus. Z Gastroenterol 2001; 39: 823-830
14. Hardt PD, Bretz L, Krauss A et al. Pathological pancreatic exocrine function and duct morphology in patients with cholelithiasis. Dig Dis Sci 2001; 46: 536-539
15. Cade A, Walters MP, McGinley N et al. Evaluation of fecal pancreatic elastase 1 as a measure of pancreatic exocrine function in children with cystic fibrosis. Pedr Pulmonol 2000; 29: 172-176
16. Gullo L, Graziano L, Babbini S et al. Faecal elastase 1 in children with cystic fibrosis. Eur J Pediatr 1997; 156: 770-772
17. Soldan W, Henker J, Sprössig C. Sensitivity and Specificity of quantitative Determination of Pancreatic Elastase 1 in Feces of Children. J Ped Gastroenterol Nutr 1997; 24: 53-55
18. Walkowiak J, Cichy WK, Herzig KH. Comparison of Fecal Elastase-1 Determination with the Secretin-Cholecystokinin Test in Patients with Cystic Fibrosis. Scand J Gastroenterol 1999; 34: 201-207
19. Wallis C, Leung T, Cubitt D et al. Stool elastase as a diagnostic test for pancreatic function in children with cystic fibrosis. Lancet 1997; 350: 1001
20. Hardt PD, Krauss A, Bretz L et al. Pancreatic exocrine function in patients with type-1 and type-2 diabetes mellitus. Acta diabetol 2000; 37; 105-110
21. Scheefers-Borchel U, Scheefers H, Arnold R et al. Pankreatische Elastase 1; Parameter für die chronische und akute Pankreatitis. Lab Med 1992; 16; 427-432
22. Van de Kamer JH, ten Bockel H, Weijers HA. Rapid method for the determination of fat in feces. J Biol Chem 1949; 177: 347-355
23. Tani T, Kawashima I, Furukawa H et al. Characterization of a silent gene for human pancreatic elastase 1: structure of the 5′-flancing region. J Biochem 1987; 101: 591-599
24. Keim V, Teich N, Moessner J. Clinical value of a new fecal elastase test for the detection of chronic pancreatitis. Clin Lab 2003; 49: 209-215