The use of clonal mRNA as an antigenic format for the detection of antigen-specific T lymphocytes in IFN-γ ELISPOT assays

Journal of Immunological Methods

Volumn 287, Issue 1-2, 2004, Pages 125-136

  Britten, Cedrik M ^a   Meyer, Ralf G ^a   Frankenberg, Nadine ^a   Huber, Christoph ^a   Wölfel, Thomas ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Antigen presenting cells; APC; CTL; Cytolytic T lymphocytes; DC; Dendritic cells; ELISPOT; ELISPOT assay; Enzyme linked immunospot; FCS; Fetal calf serum; HCMV; HCMV pp65; HS; hTyr; Human cytomegalovirus; Human serum; Human tyrosinase; K562; mRNA; Peptide; Tyrosinase

Indexed keywords

EPITOPE; GAMMA INTERFERON; HLA ANTIGEN CLASS 1; MESSENGER RNA; MONOPHENOL MONOOXYGENASE;

ALLELE; ANTIGEN PRESENTING CELL; ANTIGEN SPECIFICITY; ANTIGENICITY; ARTICLE; CHRONIC MYELOID LEUKEMIA; CONTROLLED STUDY; CYTOMEGALOVIRUS; DENDRITIC CELL; DONOR; ELECTROPORATION; ENZYME LINKED IMMUNOSPOT ASSAY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; LYMPHOCYTE SUBPOPULATION; NORMAL HUMAN; PRIORITY JOURNAL; RNA TRANSCRIPTION; T LYMPHOCYTE;

ANTIGEN-PRESENTING CELLS; CLONING, MOLECULAR; DENDRITIC CELLS; ELECTROPORATION; EPITOPES, T-LYMPHOCYTE; GENES, MHC CLASS I; HUMANS; IMMUNOENZYME TECHNIQUES; INTERFERON TYPE II; K562 CELLS; MONOPHENOL MONOOXYGENASE; PHOSPHOPROTEINS; RNA, MESSENGER; T-LYMPHOCYTES; TRANSFECTION; VIRAL MATRIX PROTEINS;

CYTOMEGALOVIRUS; HUMAN HERPESVIRUS 5;

EID: 1942520378     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2004.01.026     Document Type: Article

References (32)

1. Altman J.D., Moss P.A., Goulder P.J., Barouch D.H., McHeyzer-Williams M.G., Bell J.I., McMichael A.J., Davis M.M. Phenotypic analysis of antigen-specific T lymphocytes. Science. 274:1996;94.
2. Arthur J.F., Butterfield L.H., Roth M.D., Bui L.A., Kiertscher S.M., Lau R., Dubinett S., Glaspy J., McBride W.H., Economou J.S. A comparison of gene transfer methods in human dendritic cells. Cancer Gene Ther. 4:1997;17.
3. Barnstable C.J., Bodmer W.F., Brown G., Galfre G., Milstein C., Williams A.F., Ziegler A. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell. 14:1978;9.
4. Brichard V., Van Pel A., Wölfel T., Wölfel C., De Plaen E., Lethe B., Coulie P., Boon T. The tyrosinase gene codes for an antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas. J. Exp. Med. 178:1993;489.
5. Britten C.M., Meyer R.G., Kreer T., Drexler I., Wölfel T., Herr W. The use of HLA-A*0201-transfected K562 as standard antigen-presenting cells for CD8(+) T lymphocytes in IFN-gamma ELISPOT assays. J. Immunol. Methods. 259:2002;95.
6. Brosterhus H., Brings S., Leyendeckers H., Manz R.A., Miltenyi S., Radbruch A., Assenmacher M., Schmitz J. Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion. Eur. J. Immunol. 29:1999;4053.
7. Czerkinsky C., Andersson G., Ekre H.P., Nilsson L.A., Klareskog L., Ouchterlony O. Reverse ELISPOT assay for clonal analysis of cytokine production: I. Enumeration of gamma-interferon-secreting cells. J. Immunol. Methods. 110:1988;29.
8. Diamond D.J., York J., Sun J.Y., Wright C.L., Forman S.J. Development of a candidate HLA A*0201 restricted peptide-based vaccine against human cytomegalovirus infection. Blood. 90:1997;1751.
9. Gordillo G.M., Xia D., Mullins A.N., Bergese S.D., Orosz C.G. Gene therapy in transplantation: pathological consequences of unavoidable plasmid contamination with lipopolysaccharide. Transpl. Immunol. 7:1999;83.
10. Haas G., Plikat U., Debre P., Lucchiari M., Katlama C., Dudoit Y., Bonduelle O., Bauer M., Ihlenfeldt H.G., Jung G., Maier B., Meyerhans A., Autran B. Dynamics of viral variants in HIV-1 Nef and specific cytotoxic T lymphocytes in vivo. J. Immunol. 157:1996;4212.
11. Hebart H., Rauser G., Stevanovic S., Haenle C., Nussbaum A.K., Meisner C., Bissinger A.L., Tenzer S., Jahn G., Loeffler J., Rammensee H.G., Schild H., Einsele H. A CTL epitope from human cytomegalovirus IE1 defined by combining prediction of HLA binding and proteasomal processing is the target of dominant immune responses in patients after allogeneic stem cell transplantation. Exp. Hematol. 31:2003;966.
12. Herr W., Protzer U., Lohse A.W., Gerken G., Meyer zum Büschenfelde K.H., Wölfel T. Quantification of CD8+ T lymphocytes responsive to human immunodeficiency virus (HIV) peptide antigens in HIV-infected patients and seronegative persons at high risk for recent HIV exposure. J. Infect. Dis. 178:1998;260.
13. Herr W., Ranieri E., Gambotto A., Kierstead L.S., Amoscato A.A., Gesualdo L., Storkus W.J. Identification of naturally processed and HLA-presented Epstein-Barr virus peptides recognized by CD4(+) or CD8(+) T lymphocytes from human blood. Proc. Natl. Acad. Sci. U. S. A. 96:1999;12033.
14. Janetzki S., Song P., Gupta V., Lewis J.J., Houghton A.N. Insect cells as HLA-restricted antigen-presenting cells for the IFN-gamma ELISPOT assay. J. Immunol. Methods. 234:2000;1.
15. Jonuleit H., Kuhn U., Muller G., Steinbrink K., Paragnik L., Schmitt E., Knop J., Enk A.H. Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27:1997;3135.
16. Kern F., Surel I.P., Brock C., Freistedt B., Radtke H., Scheffold A., Blasczyk R., Reinke P., Schneider-Mergener J., Radbruch A., Walden P., Volk H.D. T-cell epitope mapping by flow cytometry. Nat. Med. 4:1998;975.
17. Konopitzky R., König U., Meyer R.G., Sommergruber W., Wölfel T., Schweighoffer T. Identification of HLA-A*0201-restricted T cell epitopes derived from the novel overexpressed tumor antigen calcium-activated chloride channel 2. J. Immunol. 169:2002;540.
18. Kuball J., Schuler M., Antunes F.E., Herr W., Neumann M., Obenauer-Kutner L., Westreich L., Huber C., Wölfel T., Theobald M. Generating p53-specific cytotoxic T lymphocytes by recombinant adenoviral vector-based vaccination in mice, but not man. Gene Ther. 9:2002;833.
19. Lacey S.F., Villacres M.C., La Rosa C., Wang Z., Longmate J., Martinez J., Brewer J.C., Mekhoubad S., Maas R., Leedom J.M., Forman S.J., Zaia J.A., Diamond D.J. Relative dominance of HLA-B*07 restricted CD8(+) T-Lymphocyte immune responses to human cytomegalovirus pp65 in persons sharing HLA-A*02 and HLA-B*07 alleles. Hum. Immunol. 64:2003;440.
20. Lennon-Dumenil A.M., Bakker A.H., Wolf-Bryant P., Ploegh H.L., Lagaudriere-Gesbert C. A closer look at proteolysis and MHC-class-II-restricted antigen presentation. Curr. Opin. Immunol. 14:2002;15.
21. Lozzio C.B., Lozzio B.B. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood. 45:1975;321.
22. Luo D., Saltzman W.M. Synthetic DNA delivery systems. Nat. Biotechnol. 18:2000;33.
23. Schmolke S., Drescher P., Jahn G., Plachter B. Nuclear targeting of the tegument protein pp65 (UL83) of human cytomegalovirus: an unusual bipartite nuclear localization signal functions with other portions of the protein to mediate its efficient nuclear transport. J. Virol. 69:1995;1071.
24. Skipper J.C., Hendrickson R.C., Gulden P.H., Brichard V., Van Pel A., Chen Y., Shabanowitz J., Wölfel T., Slingluff C.L. Jr., Boon T., Hunt D.F., Engelhard V.H. An HLA-A2-restricted tyrosinase antigen on melanoma cells results from posttranslational modification and suggests a novel pathway for processing of membrane proteins. J. Exp. Med. 183:1996;527.
25. Strobel I., Berchtold S., Gotze A., Schulze U., Schuler G., Steinkasserer A. Human dendritic cells transfected with either RNA or DNA encoding influenza matrix protein M1 differ in their ability to stimulate cytotoxic T lymphocytes. Gene Ther. 7:2000;2028.
26. Tsomides T.J., Walker B.D., Eisen H.N. An optimal viral peptide recognized by CD8+ T cells binds very tightly to the restricting class I major histocompatibility complex protein on intact cells but not to the purified class I protein. Proc. Natl. Acad. Sci. U. S. A. 88:1991;11276.
27. Van Tendeloo V.F., Ponsaerts P., Lardon F., Nijs G., Lenjou M., Van Broeckhoven C., Van Bockstaele D.R., Berneman Z.N. Highly efficient gene delivery by mRNA electroporation in human hematopoietic cells: superiority to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for tumor antigen loading of dendritic cells. Blood. 98:2001;49.
28. Wills M.R., Carmichael A.J., Mynard K., Jin X., Weekes M.P., Plachter B., Sissons J.G. The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity, and T-cell receptor usage of pp65-specific CTL. J. Virol. 70:1996;7569.
29. Wolf P.R., Ploegh H.L. How MHC class II molecules acquire peptide cargo: biosynthesis and trafficking through the endocytic pathway. Annu. Rev. Cell Dev. Biol. 11:1995;267.
30. Wölfel T., Hauer M., Klehmann E., Brichard V., Ackermann B., Knuth A., Boon T., Meyer zum Büschenfelde K.H. Analysis of antigens recognized on human melanoma cells by A2-restricted cytolytic T lymphocytes (CTL). Int. J. Cancer. 55:1993;237.
31. Wölfel T., Van Pel A., Brichard V., Schneider J., Seliger B., Meyer zum Büschenfelde K.H., Boon T. Two tyrosinase nonapeptides recognized on HLA-A2 melanomas by autologous cytolytic T lymphocytes. Eur. J. Immunol. 24:1994;759.
32. Yang S., Linette G.P., Longerich S., Haluska F.G. Antimelanoma activity of CTL generated from peripheral blood mononuclear cells after stimulation with autologous dendritic cells pulsed with melanoma gp100 peptide G209-2M is correlated to TCR avidity. J. Immunol. 169:2002;531.