Construction of a universal expression vector for human-mouse chimeric Fab antibody and expression of chimeric Fab antibody against human hepatoma associated antigen HAb18G

World Chinese Journal of Digestology

Volumn 12, Issue 2, 2004, Pages 271-275

  Xing, Jin Liang ^a   Yang, Xiang Min ^a   Zhang, Si He ^a   Yao, Xi Ying ^a   Liang, Rui An ^a   Chen, Zhi Nan ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CHIMERIC ANTIBODY; HAB18G ANTIGEN; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G3; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN KAPPA CHAIN; MONOCLONAL ANTIBODY; TUMOR ANTIGEN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANTIGEN BINDING; ANTIGEN SPECIFICITY; ARTICLE; BINDING AFFINITY; CYTOPLASM; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE EXPRESSION; GENE LOCATION; GENETIC TRANSFECTION; HUMAN; IMMUNOFLUORESCENCE; LIVER CELL CARCINOMA; MOLECULAR CLONING; MOLECULAR WEIGHT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING;

EID: 1942442245     PISSN: 10093079     EISSN: None     Source Type: Journal    
DOI: 10.11569/wcjd.v12.i2.271     Document Type: Article

References (24)

1. Lou C, Chen ZN, Bian HJ, Li J, Zhou SB. Pharmacokinetics of radioimmunotherapeutic agent of direct labeling mAb 188Re-HAb18. World J Gastroenterol 2002;8:69-73
2. Yang LJ, Sui YF, Chen ZN. Preparation and activity of conjugate of monoclonal antibody HAb18 against hepatoma F(ab′) (2) fragment and staphylococcal enterotoxin A. World J Gastroenterol 2001;7:216-221
3. Bian HJ, Chen ZN, Deng JL. Direct technetium-99m labeling of anti-hepatoma monoclonal antibody fragment:a radioimmunoconjugate for hepatocellular carcinoma imaging. World J Gastroenterol 2000;6:348-352
4. Qiu K, Wang BC, Chen ZN, Fang P, Liu CG, Wan WX, Liu YF. 99mTc-labeled HAb18 McAb Fab fragment for radioimmunoimaging in nude mice bearing human hepatocellular carcinoma. World J Gastroenterol 1998;4:117-1201
5. Hasholzner U, Stieber P, Meier W, Lamerz R. Value of HAMA - determination in clinical practice - an overview. Anticancer Res 1997;17:3055-3058
6. Souriau C, Hudson PJ. Recombinant antibodies for cancer diagnosis and therapy. Expert Opin Biol Ther 2003;3:305-318
7. He FT, Nie YZ, Chen BJ, Qiao TD, Fan DM, Li RF, Kang YS, Zhang Y. Expression and identification of recombinant soluble single-chain variable fragment of monoclonal antibody MC3. World J Gastroenterol 2002;8:258-262
8. Goldenberg DM. Advancing role of radiolabeled antibodies in the therapy of cancer. Cancer Immunol Immunother 2003;52:281-296
9. Trail PA, King HD, Dubowchik GM. Monoclonal antibody drug immunoconjugates for targeted treatment of cancer. Cancer Immunol Immunother 2003;52:328-337
10. Milenic DE. Monoclonal antibody-based therapy strategies: providing options for the cancer patient. Curr Pharm Des 2002;8:1749-1764
11. Chuntharapai A, Lai J, Huang X, Gibbs V, Kim KJ, Presta LG, Stewart TA. Characterization and humanization of a monoclonal antibody that neutralizes human leukocyte interferon: a candidate therapeutic for IDDM and SLE. Cytokine 2001;15:250-260
12. Hudson PJ, Souriau C. Engineered antibodies. Nat Med 2003;9:129-134
13. Caldas C, Coelho V, Kalil J, Moro AM, Maranhao AQ, Brigido MM. Humanization of the anti-CD18 antibody 6.7:an unexpected effect of a framework residue in binding to antigen. Mol Immunol 2003;39:941-952
14. Carter P. Improving the efficacy of antibody-based cancer therapies. Nat Rev Cancer 2001;1:118-129
15. Linenberger ML, Maloney DG, Bernstein ID. Antibody-directed therapies for hematological malignancies. Trends Mol Med 2002;8:69-76
16. Hellstrom HR. Platelet glycoprotein IIb/IIIa inhibitors. Circulation 2003;107:E39-49
17. Humphreys DP, Vetterlein OM, Chapman AP, King DJ, Antoniw P, Suitters AJ, Reeks DG, Parton TA, King LM, Smith BJ, Lang V, Stephens PE. F (ab′)2 molecules made from Escherichia coli produced Fab′ with hinge sequences conferring increased serum survival in an animal model. J Immunol Methods 1998;217:1-10
18. Maeda F, Nagatsuka Y, Ihara S, Aotsuka S, Ono Y, Inoko H, Takekoshi M. Bacterial expression of a human recombinant monoclonal antibody fab fragment against hepatitis B surface antigen. J Med Virol 1999;58:338-345
19. Raffai R, Vukmirica J, Weisgraber KH, Rassart E, Innerarity TL, Milne R. Bacterial expression and purification of the Fab fragment of a monoclonal antibody specific for the low-density lipoprotein receptor-binding site of human apolipoprotein E. Protein Expr Purif 1999;16:84-90