Identification of Actinobacillus actinomycetemcomitans using species-specific 16S rDNA primers

Journal of Microbiology

Volumn 43, Issue 2, 2005, Pages 209-212

  Kim, Su Gwan ^a   Kim, Soo Heung ^a   Kim, Mi Kwang ^a   Kim, Hwa Sook ^a   Kook, Joong Ki ^a  

^a Chosun University   (South Korea)

Author keywords

16s rdna; Actinobacillus actinomycetemcomitans; Species specific pcr primer

Indexed keywords

GENOMIC DNA; PRIMER DNA; RIBOSOME DNA; RIBOSOME RNA; RNA 16S;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; AMPLICON; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DILUTION; GENETICS; HAEMOPHILUS; ISOLATION AND PURIFICATION; METHODOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; DNA PRIMERS; DNA, RIBOSOMAL; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; HAEMOPHILUS;

EID: 19344369871     PISSN: 12258873     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Ashimoto, A., C. Chen, I. Bakker, and J. Slots. 1996. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol. Immunol. 11, 266-273.
2. Ashkenazi, M., R.R. White, and O.K. Dennison. 1992. Neutrophil modulation by Actinobacillus actinomycetemcomitans. I. Chemotaxis, surface receptor expression and F-actin polymerization. J. Periodontal Res. 27, 264-273.
3. Asikainen, S. 1986. Occurrence of Actinobacillus actinomycetemcomitans and spirochetes in relation to age in localized juvenile periodontitis. J. Periodontol. 57, 537-541.
4. Conrads, G., T.F. Flemmig, I. Seyfarth, F. Lampert, and R. Lutticken. 1999. Simultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR. J. Clin. Microbiol. 37, 1621-1624.
5. Darveau, R.P., A. Tanner, and R.C. Page. 1997. The microbial challenge in periodontitis. Periodontol. 2000 14, 12-32.
6. Genco, R.J., J.J. Zambon, and P.A. Murray. 1985. Serum and gingival fluid antibodies as adjuncts in the diagnosis of Actinobacillus actinomycetemcomitans-associated periodontal disease. J. Periodontol. 56, 1-50.
7. Kiley, P. and S.C. Holt. 1980. Characterization of the lipopolysaccharide from Actinobacillus actinomycetemcomitans Y4 and N27. Infect. Immun. 30, 862-873.
8. Kook, J.-K., M.-K. Kim, J.-H. Seong, D.-K. Kim, B.-O. Kim, J.-C. Park, K.-K. Kim, S.-J. Choe, and B.-M. Min. 2003. A new method for rapid screening of bacterial species- or subspecies-specific DNA probes. FEMS Microbiol. Lett. 219, 121-127.
9. Lally, E.T., I.R. Kieba, D.R. Demuth, J. Rosenbloom, E.E. Golub, N.S. Taichman, and C.W. Gibson. 1989. Identification and expression of the Actinobacillus actinomycetemcomitans leukotoxin gene. Biochem. Biophys. Res. Commun. 159, 256-262.
10. Lippke, J.A., M.N. Strzempko, F.F. Raia, S.L. Simon, and C.K. French. 1987. Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate. Appl. Environ. Microbiol. 53, 2588-2589.
11. Mandell, R.L. 1984. A longitudinal microbiological investigation of Actinobacillus actinomycetemcomitans and Eikenella corrodens in juvenile periodontitis. Infect. Immun. 45, 778-780.
12. Robertson, P.B., M. Lantz, P.T. Marucha, K.S. Kornman, C.L. Trammel, and S.C. Holt. 1982. Collagenolytic activity associated with Bacteroides species and Actinobacillus actinomycetemcomitans. J. Periodontal Res. 17, 275-283.
13. Seong, J.H., D.K. Kim, K.Y. Shin, B.O. Kim, M.S. Kim, M.K. Kim, and J.K. Kook. 2003. Simultaneous detection of Prevotella intermedia and Prevotella nigrescens by 16S rDNA-directed multiplex PCR. J. Korean Acad. Dent. Health 26, 523-533.
14. Slots, J., A. Ashimoto, M.J. Flynn, G. Li, and C. Chen. 1995. Detection of putative periodontal pathogens in subgingival specimens by 16S ribosomal DNA amplification with the polymerase chain reaction. Clin. Infect. Dis. 20(Suppl 2), S304-307.
15. Sugai, M., T. Kawamoto, S.Y. Peres, Y. Ueno, H. Komatsuzawa, T. Fujiwara, H. Kurihara, H. Suginaka, and E. Oswald. 1998. The cell cycle-specific growth-inhibitory factor produced by Actinobacillus actinomycetemcomitans is a cytolethal distending toxin. Infect. Immun. 66, 5008-5019.
16. Tonjum, T., and R. Haas. 1993. Identification of Actinobacillus actinomycetemcomitans by leukotoxin gene-specific hybridization and polymerase chain reaction assays. J. Clin. Microbiol. 31, 1856-1859.
17. Tran, S.D. and J.D. Rudney. 1996. Multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. J. Clin. Microbiol. 34, 2674-2678.
18. Tran, S.D. and J.D. Rudney. 1999. Improved multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis. J. Clin. Microbiol. 37, 3504-3508.
19. van Winkelhoff, A.J. and J. Slots. 1999. Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in nonoral infections. Periodontol. 2000 20, 122-135.
20. Woese, C.R. 1987. Bacterial evolution. Microbiol. Rev. 51, 221-271.
21. Zambon, J.J. 1985. Actinobacillus actinomycetemcomitans in human periodontal disease. J. Clin. Periodontol. 12, 1-20.