Recovery of upstream cDNA sequences by a PCR-based biotin-capture method

BioTechniques

Volumn 27, Issue 3, 1999, Pages 488-498

  Graslund S ^a   Larsson, M ^a   Sterky, F ^a   Uhlen M ^a   Lundeberg, J ^a   Hoog C ^b   Stahl, Stefan ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN;

ANIMAL TISSUE; ARTICLE; DNA SEQUENCE; GENE AMPLIFICATION; GENE ISOLATION; GENE TARGETING; MOLECULAR CLONING; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION;

ANIMALIA;

EID: 18844481785     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Bertling, W.M., F. Beier and E. Reichenberger. 1993. Determination of 5′ ends of specific mRNAs by DNA ligase-dependent amplification. PCR Methods Appl. 3:95-99.
2. Carninci, P., A. Westover, Y. Nishiyama, T. Ohsumi, M. Itoh, S. Nagaoka, N. Sasaki, Y. Okazaki et al. 1997. High efficiency selection of full-length cDNA by improved biotinylated cap trapper. DNA Res. 4:61-66.
3. Chenchik, A., L. Diachenko, F. Moqadam, V. Tarabykin, S. Lukyanov and P.D. Seibert 1996. Full-length cDNA cloning and determination of mRNA 5′ and 3′ ends by amplification of adaptor-ligated cDNA. BioTechniques 21:526-534.
4. Edery, I., L.L. Chu, N. Sonenberg and J. Pelletier. 1995. An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture). Mol. Cell. Biol. 15:3363-3371.
5. Edwards, J.B., J. Delort and J. Mallet. 1991. Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5′ ends of mRNAs and for constructing cDNA libraries by in vitro amplification. Nucleic Acids Res. 19:5221-5232.
6. Fors, L., R.A. Saavedra and L. Hood. 1990. Cloning of the shark Po promoter using a genomic walking technique based on the polymerase chain reaction. Nucleic Acids Res. 18:2793-2799.
7. Frohman, M.A., M.K. Dush and G.R. Martin. 1988. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85:8998-9002.
8. Fromont-Racine, M., E. Bertrand, R. Pictet and T. Grange. 1993. A highly sensitive method for mapping the 5′ termini of mRNAs. Nucleic Acids Res. 21:1683-1684.
9. Hakvoort, T.B., J.A. Spijkers, J.L. Vermeulden and W.H. Lamers. 1996. Preparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand formation with subtractively enriched cDNA fragments. Nucleic Acids Res. 24:3478-3480.
10. Höög, C. 1991. Isolation of a large number of novel mammalian genes by a differential cDNA library screening strategy. Nucleic Acids Res. 19:6123-6127.
11. Lagerström, M., J. Parik, H. Malmgren, J. Stewart, U. Pettersson and U. Landegren. 1991. Capture PCR: efficient amplification of DNA fragments adjacent to a known sequence in human and YAC DNA. PCR Methods Appl. 1:111-119.
12. Larsson, M., E. Brundell, P.-M. Jörgensen, S. Ståhl and C. Höög. A novel nudeolar protein that associates with the condensed chromosomes in mitotic cells and with the centro-somes of haploid cells. Eur. J. Cell Biol. (In press).
13. Liu, X. and M.A. Gorovsky. 1993. Mapping the 5′ and 3′ ends of Tetrahymena thermophila mRNAs using RNA ligase mediated amplification of cDNA ends (RLM-RACE). Nucleic Acids Res. 21:4954-4960.
14. Loh, E.V., J.F. Elliott, S. Cwlrla, L.L. Lanler and M.M. Davis. 1989. Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain. Science 243:217-220.
15. Nguyen, T.N., P. Samuelson, F. Sterky, C. Merle-Poitte, A. Robert, T. Baussant, J.F. Haeuw, M. Uhlén, H. Binz and S. Ståhl. 1998. Chromosomal sequencing using a PCR-based biotin-capture method allowed isolation of the complete gene for the outer membrane protein A of Klebsiella pneumoniae. Gene 210:93-101.
16. Ochman, H., A.S. Gerber and D.L. Hartl. 1988. Genetic applications of an inverse polymerase chain reaction. Genetics 120:621-623.
17. Ohara, O., R.L. Dorit and W. Gilbert. 1989. One-sided polymerase chain reaction: the amplification of cDNA. Proc. Natl. Acad. Sci. USA 86:5673-5677.
18. Parker, J.D., P.S. Rabinovitch and G.C. Burmer. 1991. Targeted gene walking polymerase chain reaction. Nucleic Acids Res. 19:3055-3060.
19. Rosenthal, A., M. Platzer and D.S. Charnock-Jones. 1994. Capture PCR: an efficient method for walking along chromosomal DNA and cDNA, p. 222-229. In K.B. Mullis, F. Ferré and R.A. Gibbs (Eds.), The Polymerase Chain Reaction. Birkhauser, Boston.
20. Schaefer, B.C. 1995. Revolutions in rapid amplification of cDNA ends: new strategies for polymerase chain reaction cloning of full-length cDNA ends. Anal. Biochem. 227:255-273.
21. Shapiro, M.B. and P. Senapathy. 1987. RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression. Nucleic Acids Res. 15:7155-7174.
22. Sterky, F., A. Holmberg, G. Alexandersson, J. Lundeberg and M. Uhlén. 1998. Direct sequencing of bacterial artificial chromosomes (BACs) and prokaryotic genomes by biotin-capture PCR. J. Biotechnol. 60:119-129.
23. Troutt, A.B., M.G. McHeyzer-Williams, B. Pulendran and G.J. Nossal. 1992. Ligation-anchored PCR: a simple amplification technique with single-sided specificity. Proc. Natl. Acad. Sci. USA 89:9823-9825.
24. Uhlén, M., T. Hultman, J. Wahlberg, J. Lundeberg, S. Bergh, B. Pettersson, A. Holmberg, S. Ståhl and T. Moks. 1992. Semi-automated solid-phase DNA sequencing. Trends Biotechnol. 10:52-55.