Creation and genetic restoration of Erwinia amylovora strains with low levan synthesis

Physiological and Molecular Plant Pathology

Volumn 65, Issue 3, 2004, Pages 115-122

  Du, Z ^a,c   Jakovljevic, V ^a   Salm, H ^a   Geider, K ^a,b  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b INSTITUTE FOR EPIDEMIOLOGY AND PATHOGEN DIAGNOSTICS   (Germany)

^c INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

Author keywords

Fire blight; Gene regulation; Levansucrase; Phenotypical restoration; rls genes

Indexed keywords

ERWINIA; ERWINIA AMYLOVORA; ERWINIA PYRIFOLIAE;

EID: 18844452429     PISSN: 08855765     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pmpp.2004.12.003     Document Type: Article

References (34)

1. P. Aldridge, M. Metzger, and K. Geider Genetics of sorbitol metabolism in Erwinia amylovora and its influence on bacterial virulence Mol Gen Genet 256 1997 611 619
2. J. Arrieta, L. Hernández, A. Coego, V. Suárez, E. Balmori, and C. Menéndez Molecular characterization of the levansucrase gene from the endophytic sugarcane bacterium Acetobacter diazotrophicus SRT4 Microbiology 142 1996 1077 1085
3. M.A. Barny, M.H. Guinebretiere, B. Marcais, E. Coissac, J.P. Paulin, and J. Laurent Cloning of a large gene cluster involved in Erwinia amylovora CFBF 1430 virulence Mol Microbiol 4 1990 777 786
4. P. Bellemann, and K. Geider Localization of transposon insertions in pathogenicity mutants of Erwinia amylovora and their biochemical characterization J Gen Microbiol 138 1992 931 940
5. P. Bellemann, S. Bereswill, S. Berger, and K. Geider Visualization of capsule formation by Erwinia amylovora and assays to determine amylovoran synthesis Int J Biol Macromol 16 1994 290 296
6. S. Bereswill, and K. Geider Characterization of the rcsB gene from Erwinia amylovora and its influence on exopolysaccharide synthesis and virulence of the fire blight pathogen J Bacteriol 179 1997 1354 1361
7. S. Bereswill, S. Jock, F. Aldridge, J.D. Janse, and K. Geider Molecular characterization of natural Erwinia amylovora strains deficient in levan synthesis Physiol Mol Plant Pathol 51 1997 215 225
8. F. Bernhard, K. Poetter, K. Geider, and D. Coplin The rcsA gene of Erwinia amylovora: identification, nucleotide sequence, and regulation of exopolysaccharide biosynthesis Mol Plant-Microbe Interact 3 1990 429 437
9. J. Bogs, and K. Geider Molecular analysis of sucrose metabolism of Erwinia amylovora and influence on bacterial virulence J Bacteriol 182 2000 5351 5358
10. A. Chatterjee, W. Chun, and A.K. Chatterjee Isolation and characterization of an rcsA-like gene of Erwinia amylovora that activates extracellular polysaccharide production in Erwinia species, Escherichia coli and Salmonella typhimurium Mol Plant-Microbe Interact 3 1990 144 148
11. l-homoserine lactone Appl Environ Microbiol 61 1995 1959 1967
12. C. Chong Study of seasonal and daily distribution of sorbitol and related carbohydrates within apple seedlings by analysis of selected tissue and organs Can J Plant Sci 51 1971 519 525
13. M. Coleman, R. Pearce, F. Hitchin, F. Busfield, J.W. Mansfield, and I.S. Roberts Molecular cloning, expression and nucleotide sequence of the rcsA gene of Erwinia amylovora encoding a positive regulator of capsule expression: evidence for a family of related capsule activator proteins J Gen Microbiol 136 1990 1799 1806
14. P. Dersch, K. Schmidt, and E. Bremer Synthesis of Escherichia coli K-12 nucleoid-associated DNA-binding protein H-NS is subjected to growth phase control and autoregulation Mol Microbiol 8 1993 875 889
15. Z. Du, and K. Geider Characterization of an activator gene upstream of lsc, involved in levan synthesis of Erwinia amylovora Physiol Mol Plant Pathol 60 2002 9 17
16. H. Falkenstein, P. Bellemann, S. Walter, W. Zeller, and K. Geider Identification of, the fire blight pathogen Erwinia amylovora, by colony hybridization with DNA from plasmid pEA29 Appl Environ Microbiol 54 1988 2798 2802
17. K. Geider, C. Hohmeyer, R. Haas, and T.F. Meyer A plasmid cloning system utilizing replication functions of the filamentous bacteriophage fd Gene 33 1985 341 349
18. G. Geier, and K. Geider Characterization and influence on virulence of the levansucrase gene from the fire blight pathogen Erwinia amylovora Physiol Mol Plant Pathol 42 1993 387 404
19. M. Gross, G. Geier, K. Rudolph, and K. Geider Levan and levansucrase synthesized by the fire blight pathogen Erwinia amylovora Physiol Mol Plant Pathol 40 1992 371 381
20. U. Hettwer, F.R. Jaeckel, J. Boch, M. Meyer, K. Rudolph, and M.S. Ullrich Cloning, nucleotide sequence, and expression in Escherichia coli of levansucrase genes from the plant pathogens Pseudomonas syringae pv. glycinea and P. syringae pv. phaseolicola Appl Environ Microbiol 64 1998 3180 3187
21. J.F. Kim, and S.V. Beer hrp genes and harpins of Erwinia amylovora: a decade of discovery J. Vanneste Fire blight: the disease and its causative agent Erwinia amylovora 2000 Cabi Publishing Wallingford 141 161
22. W.S. Kim, L. Gardan, S.L. Rhim, and K. Geider Erwinia pyrifoliae sp. nov., a novel pathogen that affects asian pear trees (Pyrus pyrifolia Nakai) Int J Syst Bacteriol 49 1999 899 906
23. W.-S. Kim, M. Hildebrand, S. Jock, and K. Geider Molecular comparison of pathogenic bacteria from pear trees in Japan and the fire blight pathogen Erwinia amylovora Microbiology/SGM 147 2001 2951 2959
24. J.A. Leigh, and D.L. Coplin Exopolysaccharides in plant-bacterial interactions Annu Rev Microbiol 46 1992 307 346
25. Y. Li, J.A. Triccas, and T. Ferenci A novel levansucrase-levanase gene cluster in Bacillus stearothermophilus ATCC12980 Biochim Biophys Acta 1353 1997 203 208
26. C. Manoil, and J. Beckwith TnphoA: a transposon probe for protein export signals Proc Natl Acad Sci USA 82 1985 8129 8133
27. M. Metzger, P. Bellmann, T. Schwartz, and K. Geider Site-directed and transposon-mediated mutagenesis with pfd-plasmids by electroporation of Erwinia amylovora and Escherichia coli cells Nucleic Acids Res 20 1992 2265 2270
28. K. Poetter, and D.L. Coplin Structural and functional analysis of the rcsA gene from Erwinia stewartii Mol Gen Genet 229 1991 155 160
29. J. Sambrook, and W. Rusell Molecular cloning: a laboratory manual 3rd ed. 2001 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
30. R. Simon, U. Priefer, and A. Pühler A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria Bio/Technology 1 1983 784 791
31. V. Stout, A. Torres-Cabassa, M.R. Maurizi, D. Gutznick, and S. Gottesman RcsA, an unstable positive regulator of capsular polysaccharide synthesis J Bacteriol 173 1991 1738 1747
32. L.B. Tang, R. Lenstra, T.V. Borchert, and V. Nagarajan Isolation and characterization of levansucrase-encoding gene from Bacillus amyloliquefaciens Gene 96 1990 89 93
33. T. Van der Zwet, and H.L. Keil Fire blight: a bacterial disease of rosaceous plants Agriculture handbook no. 510 1979 US Government Printing Office Washington p. 200
34. Y. Zhang, and K. Geider Molecular analysis of the rlsA gene regulating levan production by the fireblight pathogen Erwinia amylovora Physiol Mol Plant Pathol 54 1999 187 201