Genetics of sorbitol metabolism in Erwinia amylovora and its influence on bacterial virulence

Molecular and General Genetics

Volumn 256, Issue 6, 1997, Pages 611-619

  Aldridge, P ^a   Metzger, M ^a   Geider, K ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

Carbohydrate uptake; Erwinia amylovora; Fire blight; srl gut operon; Virulence

Indexed keywords

DNA FRAGMENT; SORBITOL;

APPLE; ARTICLE; BACTERIAL GENETICS; BACTERIAL METABOLISM; BACTERIAL VIRULENCE; CARBOHYDRATE TRANSPORT; CULTURE MEDIUM; ERWINIA; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; NONHUMAN; OPEN READING FRAME; OPERON; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; DNA, BACTERIAL; ERWINIA; ESCHERICHIA COLI; FRUCTOSE; FRUIT; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENETIC COMPLEMENTATION TEST; GLUCOSE; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; OPERON; PLANT DISEASES; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SORBITOL; SPECIES SPECIFICITY; TRANSCRIPTION, GENETIC; VIRULENCE;

EID: 0030712282     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050609     Document Type: Article

References (47)

1. Bachman B (1990) Linkage map of Escherichia coli K-12. Microbiol Rev 54:130 197
2. Barny MA, Guinebretiere MH, Marcais B, Coissac E, Paulin JP, Laurent J (1990) Cloning of a large gene cluster involved in Erwinia amylovora CFBP1430 virulence. Mol Microbiol 4:777-786
3. Bauer DW, Beer SV (1991) Further characterization of an hrp gene cluster of Erwinia amylovora. Mol Plant-Microbe Interact 4:493-499
4. Bellemann P, Geider K (1992) Localization of transposon insertions in pathogenicity mutants of Erwinia amylovora and their biochemical characterization. J Gen Microbiol 138:931-940
5. Bellemann P, Bereswill S, Berger S, Geider K (1994) Visualization of capsule formation by Erwinia amylovora and assays to determine amylovoran synthesis. Int J Biol Macromol 16:290-296
6. Bennett RA, Billing E (1978) Capsulation and virulence in Erwinia amylovora. Annals Appl Biol 89:41-45
7. Bereswill S, Pahl A, Bellemann P, Zeller W, Geider K (1992) Sensitive and species-specific detection of Erwinia amylovora by polymerase chain reaction analysis. Appl Environ Microbiol 58:3522-3526
8. Berg DE (1977) Insertion and excision of the transposable kanamycin resistance determinant Tn5. pp 205-212. In: Bukhari AI, Shapiro JA, Adhya SL (eds). DNA insertion elements, plasmids, and episomes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
9. Berg DE, Weiss A, Crossland L (1980) Polarity of Tn5 insertion mutations in Escherichia coli. J Bacteriol 142:439-446
10. Bernhard F, Coplin DL, Geider K (1993) A gene cluster for amylovoran synthesis in Erwinia amylovora: characterization and relationship to cps genes in Erwinia stewartii. Mol Gen Genet 239:158 168
11. Bieleski RL, Redgwell RJ (1980) Sorbitol metabolism in nectraries from flowers of Rosaceae. Austral J Plant Physiol 7:15 25
12. Bugert P, Geider K (1995) Molecular analysis of the ams operon required for exopolysaccharide synthesis of Erwinia amylovora. Mol Microbiol 15:917-933
13. Chong C, Tapper CD (1971) Daily variation of sorbitol and related carbohydrates in Malux leaves. Can J Bot 49:173 177
14. Csonka LN, Clark AJ (1979) Deletions generated by the transposon Tn10 in the srl-recA region of the Escherichia coli K-12 chromosome. Genetics 93:321-343
15. Falkenstein H, Bellemann P, Walter S, Zeller W, Geider K (1988) Identification of Erwinia amylovora, the fireblight pathogen, by colony hybridization with DNA from plasmid pEA29. Appl Environ Microbiol 54: 2798 2802
16. Geider K, Hohmeyer C, Haas R, Meyer TF (1985) A plasmid cloning system utilizing replication functions of the filamentous bacteriophage fd. Gene 33:341 349
17. Hirsch PR, Beringer JE (1984) A physical map of pPHlJI and pJB4JI. Plasmid 12:139-141
18. Knauf VC, Nester EW (1982) Wide host-range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid. Plasmid 8:45 54
19. Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105-132
20. Lengeler J (1975) Nature and properties of hexitol transport systems in E. coli. J Bacteriol 124:39-47
21. Lengeler J, Lin ECCC (1972) Reversal of the mannitol-sorbitol diauxie in Escherichia coli. J Bacteriol 112:840-848
22. Lengeler JW, Jahreis K, Wehmeier (1994) Enzymes II of the phosphoenolpyruvate-dependent phosphotransferase systems: their structure and function in carbohydrate transport. Biochem Biophys Acta 1188:1-28
23. Metzger M, Bellemann P, Schwartz T, Geider K (1992) Site-directed and transposon-mediated mutagenesis with pfd-plasmids by electroporation of Erwinia amylovora and Escherichia coli cells. Nucleic Acids Research. 20:2265-2270
24. Metzger M, Bellemann P, Bugert P, Geider K (1994) Genetics of galactose metabolism of Erwinia amylovora and its influence on polysaccharide-synthesis and virulence of the fire blight pathogen. J Bacteriol 176:450-459
25. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
26. Nimtz M, Mort A, Domke T, Wray V, Zhang Y, Qiu F, Coplin D, Geider K (1996) Structure of amylovoran, the capsular exopolysaccharide from the fire blight pathogen Erwinia amylovora. Carbohydr Res 287: 59-76
27. Postma PW, Lengeler JW, Jacobson GR (1993) Phosphoenolpyruvate: carbohydrate phosphotransferase systems of bacteria. Microbiol Rev 57:543 594
28. Raese JT, Williams MW, Billingsley HD (1978) Cold hardiness, sorbitol and sugar levels of apple shoots as influenced by controlled temperature and season. J Amer Soc Hortic Sci 103:796-801
29. Saier MH Jr (1985) Mechanism and regulation of carbohydrate transport in bacteria. Academic Press, New York
30. Saier MH Jr, Chauvaux S, Deutscher J, Reizer J, Ye JJ (1995) Protein phosphorylation and regulation of carbon metabolism in Gram-negative versus Gram-positive bacteria. Trends Biochem Sci 20:267-271
31. Sancar A, Rupp WD (1979) Physical map of the recA. gene. Proc Natl Acad Sci USA 76:3144-3148
32. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
33. Sakai A (1966) Seasonal variations in the amount of polyhydric alcohol and sugar in fruit trees. J Hortic Sci 41:207-213
34. Simon R, Priefer U, Pühler A (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio/Technology 1:784-791
35. Simon R, Quandt J, Klipp W (1989) New derivatives of transposon Tn5 for mobilization of replicons, generation of operon fusions and induction of genes in Gram-negative bacteria. Gene 80:161-169
36. Spratt BG, Hedge PJ, te Heesen S, Edelman A, Broome-Smith JK (1986) Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9. Gene 41:337-342
37. Steinberger EM, Beer SV (1988) Creation and complementation of pathogenicity mutants of Erwinia amylovora. Mol Plant-Microbe Interact 1:135-144
38. Suleman P, Steiner PW (1994) Relationship between sorbitol and solute potential in apple shoots relative to fire blight symptom development after infection by Erwinia amylovora. Phytopathology 84:1244-1250
39. Wallaart RAM (1980) Distribution of sorbitol in Rosaceae. Phytochemistry 19:2603-2610
40. Wierenga RK, Terpstra P, Hol WGJ (1986) Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprint. J Mol Biol 187:101-107
41. Willis DK, Uhlin BE, Amini KS, Clark AJ (1981) Physical mapping of the srl recA region of Escherichia coli: analysis of Tn10 generated insertions and deletions. Mol Gen Genet 183:497-504
42. Winkler HH (1971) Efflux and steady state in α-methylglucoside transport in E. coli. J Bacteriol 106:362-368
43. Yamada M, Saier MH Jr (1987a) Physical and genetic characterization of the glucitol operon in Escherichia coli. Nucleotide sequence of the gut operon. J Bacteriol 169:2990-2994
44. Yamada M, Saier MH Jr (1987b) Glucitol-specific enzymes of the phosphotransferase system in Escherichia coli. J Biol Chem 262:5455-5463
45. Yamada M, Feucht BU, Saier Jr MH (1987) Regulation of gluconeogenesis by the glucitol enzyme III of the phosphotransferase system in Escherichia coli. J Bacteriol 169:5416-5422
46. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119
47. Zimmermann MH, Ziegler H (1975) List of sugars and sugar alcohols in sieve-tube exudates. In: Pirson A. Zimmermann MH (eds). Encyclopedia of plant physiology New series, vol 1. Springer Verlag, Berlin-Heidelberg-New York, pp 480-503