Dynamic tracking and mobility analysis of single GLUT4 storage vesicle in live 3T3-L1 cells

Cell Research

Volumn 14, Issue 6, 2004, Pages 480-486

  Li, Chen Hong ^a   Bai, Li ^a   Li, Dong Dong ^a   Xia, Sheng ^a   Xu, Tao ^a,b  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b INSTITUTE OF BIOPHYSICS   (China)

Author keywords

3T3 L1; Fluorescence microscopy; GLUT4; GLUT4 storage vesicle (GSV); Insulin; Single particle tracking; Total internal reflection

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GLUCOSE TRANSPORTER; GLUCOSE TRANSPORTER 4; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MUSCLE PROTEIN; SLC2A4 PROTEIN, MOUSE;

ANIMAL; ARTICLE; CELL STRAIN 3T3; CONFOCAL MICROSCOPY; CYTOPLASM VESICLE; DIFFUSION; GENETICS; METABOLISM; METHODOLOGY; MOUSE; MOVEMENT (PHYSIOLOGY); NORMAL DISTRIBUTION; PARTICLE SIZE; PHYSIOLOGY; PROTEIN TRANSPORT; SPECTROFLUOROMETRY; TIME;

3T3-L1 CELLS; ANIMALS; CYTOPLASMIC VESICLES; DIFFUSION; GLUCOSE TRANSPORTER TYPE 4; GREEN FLUORESCENT PROTEINS; MICE; MICROSCOPY, CONFOCAL; MONOSACCHARIDE TRANSPORT PROTEINS; MOVEMENT; MUSCLE PROTEINS; NORMAL DISTRIBUTION; PARTICLE SIZE; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; TIME FACTORS;

EID: 18844436364     PISSN: 10010602     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cr.7290251     Document Type: Article

References (37)

1. Bryant NJ, Govers R, James D. Regulated transport of the glucose transporter glut4. Nat Rev Mol Cell Biol 2002; 3: 267-77
2. Petersen KF, Shulman GI. Pathogenesis of skeletal muscle insulin resistance in type 2 diabetes mellitus. Am J Cardiol 2002; 90: 11G-18G..
3. Hirohito Sone, Hiroaki Suzuki, Akimitsu Takahashi and Nobuhiro Yamada Disease model: hyperinsulinemia and insulin resistance. TRENDS in Molecular Medicine 2001; 7:320-2.
4. Garvey WT. Glucose transport and NIDDM.Diabetes Care 1992; 15:396-417.
5. Chiang SH, Baumann CA, Kanzaki M, et al. Insulin-stimulated GLUT4 translocation requires the CAP-dependent activation of TC10. Nature 2001; 410:944-8
6. Bogan JS, Hendon N, McKee AE, Tsao TS, Lodish HF. Functional cloning of TUG as a regulator of GLUT4 glucose transporter trafficking. Nature 2003; 425:727-34
7. Lampson MA, Schmoranzer J, Zeigerer A, Simon SM, McGraw TE. Insulin-regulated Release from the Endosomal Recycling Compartment Is Regulated by Budding of Specialized Vesicles. Mol Biol Cell 2001; 12:3489-501
8. Zeigerer A, Lampson MA, Karylowski O, et al. GLUT4 retention in adipocytes requires two intracellular insulin-regulated transport steps. Mol Biol Cell 2002; 13:2421-35
9. Karylowski O, Zeigerer A, Cohen A, McGraw TE. GLUT4 is retained by an intracellular cycle of vesicle formation and fusion with endosomes. Mol Biol Cell 2004; 15:870-82
10. Molloy SS, Anderson ED, Jean F, Thomas G. Bi-cycling the furin pathway: from TGN localization to pathogen activation and embryogenesis. Trends Cell Biol 1999; 9:28-35
11. Xiang Y, Molloy SS, Thomas L, Thomas G. The PC6B cytoplasmic domain contains two acidic clusters that direct sorting to distinct trans-Golgi network/endosomal compartments. Mol Biol Cell 2000; 11:1257-73
12. Martin S, Ramm G, Lyttle CT, et al. Biogenesis of insulin-responsive GLUT4 vesicles is independent of brefeldin A-sensitive trafficking. Traffic 2000; 1:652-60
13. Palacios S, Lalioti V, Martinez-Arca S, Chattopadhyay S, Sandoval IV. Recycling of the insulin-sensitive glucose transporter GLUT4. Access of surface internalized GLUT4 molecules to the perinuclear storage compartment is mediated by the Phe5-Gln6-Gln7-Ile8 motif. J Biol Chem 2001; 276:3371-83
14. Jahn R, Lang T, Südhof TC. Membrane Fusion. Cell 2003; 112: 519-33.
15. Hashiramoto M, James DE. Characterization of insulin-responsive Glut4 vesicles isolated from 3T3-L1 adipocytes. Mol Cell Biol 2000; 20:416-427
16. Ramm G, Slot JW, James DE, Stoorvogel W. Insulin recruits GLUT4 from specialized VAMP2-carrying vesicles as well as from the dynamic endosomal/trans-Golgi network in rat adipocytes. Mol Biol Cell 2000; 11:4079-91
17. Cheezum MK, Walker WF, Guilford WH. Quantitative comparison of algorithms for tracking single fluorescent particles. Biophys J 2001; 81:2378-88
18. Kues T, Dickmanns A, Luhrmann R, Peters R, Kubitscheck U. High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking. Proc Natl Acad Sci U S A 2001; 98:12021-6
19. Goulian M, Simon SM. Tracking single proteins within cells. Biophys J 2000; 79:2188-98
20. Thompson RE, Larson DR, Webb WW. Precise nanometer localization analysis for individual fluorescent probes. Biophys J 2002; 82:2775-83
21. Ölveczky BP, Periasamy N, Verkman AS. Mapping fluorophore distributions in three dimensions by quantitative multiple angle-total internal reflection fluorescence microscopy. Biophys J 1997; 73:2836-47
22. Steyer JA, Almers W. A real-time view of life within 100 nm of the plasma membrane. Nat Rev Mol Cell Biol, 2001; 2:268-75
23. Oheim M, Stühmer M. Tracking chromaffin granules on their way through the actin cortex. Eur Biophys J, 2000; 29:67-89
24. Rizzuto R, Carrington W, Tuft RA. Digital imaging microscopy of living cells. Trends Cell Biol 1998; 8:288-92
25. Steyer JA, Almers W. Tracking single secretory granules in live chromaffin cells by evanescent-field fluorescence microscopy. Biophys J 1999; 76:2262-71
26. Becherer U, Moser T, Stuhmer W, Oheim M. Calcium regulates exocytosis at the level of single vesicles. Nat Neurosci 2003, 6: 846-53.
27. Lang T, Wacker I, Wunderlich I, et al. Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells. Biophys J 2000; 78:2863-77
28. Qian H, Sheetz MP, Elson EL. Single particle tracking. Analysis of diffusion and flow in two-dimensional systems. Biophys J 1991; 60:910-21
29. Saxton MJ, Jacobson K. Single-particle tracking: applications to membrane dynamics. Annu Rev Biophys Biomol Struct 1997; 26:373-99
30. Saxton MJ. Single-particle tracking: the distribution of diffusion coefficients. Biophys J 1997; 72:1744-53
31. Ng YK, Lu X, Gulacsi A, et al. Unexpected mobility variation among individual secretory vesicles produces an apparent refractory neuropeptide pool. Biophys J 2003; 84:4127-34
32. Liu LB, Omata W, Kojima I, Shibata H. Insulin recruits GLUT4 from distinct compartments via distinct traffic pathways with differential microtubule dependence in rat adipocytes. J Biol Chem 2003; 278:30157-69
33. Johns LM, Levitan ES, Shelden EA, Holz RW, Axelrod D. Restriction of secretory granule motion near the plasma membrane of chromaffin cells. J Cell Biol 2001; 153(4):177-90
34. Hirokawa N. Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science 1998; 279:519-26
35. Kamal A, Goldstein LS. Principles of cargo attachment to cytoplasmic motor proteins. Curr Opin Cell Biol 2002; 14:63-8
36. Thorn, NA. In vitro studies of the release mechanism for vasopressin in rats. Acta Endocrinol 1966; 53:644-54.
37. Burke NV, Han W, Li D, et al. Neuronal peptide release is limited by secretory granule mobility. Neuron 1997;19:1095-102.