Development of a Nonradioactive, Time-Resolved Fluorescence Assay for the Measurement of Jun N-Terminal Kinase Activity

Journal of Biomolecular Screening

Volumn 2, Issue 4, 1997, Pages 213-223

  Gaarde, William A ^a,b   Hunter, Tony ^c   Brady, Helen ^a   Murray, Brion W ^a   Goldman, Mark E ^a,d  

^a CELGENE CORPORATION   (United States)

^b ISIS PHARMACEUTICALS   (United States)

^c SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^d Axiom Biotechnologies Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 18844420062     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/108705719700200406     Document Type: Article

References (19)

1. Karin, M., Hunter, T. (1995). Transcriptional control by protein phosphorylation: Signal transmission from the cell surface to the nucleus. Curr. Biol. 5: 747-757.
2. Stein, B., Anderson, D. (1996). The MAP kinase family: New “MAPs” for signal transduction pathways and novel targets for drug discovery. In: Annual Reports in Medicinal Chemistry. J.A. Bristol (ed), 289-298. Academic Press, San Diego, CA.
3. Hibi, M., Lin, A., Smeal, T., Minden, A., Karin, M. (1993). Identification of an oncoprotein- and UV-responsive protein kinase that binds and potentiates the c-Jun activation domain. Genes Dev. 7: 2135-2148.
4. Derijard, B., Hibi, M., Wu, I.H., Barrett, T., Su, B., Deng, T., Karin, M., Davis, R.J. (1994). A protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell 76: 1025-1037.
5. Adler, J., Schaffer, A., Kim, J., Dolan, L., Ronai, Z. (1995). UV irradiation and heat shock mediate JNK activation via alternate pathways. J. Biol. Chem. 270: 26071-26077.
6. Hambleton, J., Weinstein, S.L., Lem, L., DeFranco, A.L. (1996). Activation of c-Jun N-terminal kinase in bacterial lipopolysac-charide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778.
7. Kallunki, T., Su, B., Tsigelny, I., Sluss, H.K., Derijard, B., Moore, G., Davis, R., Karin, M. (1994). JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation. Genes Dev. 8: 2996-3007.
8. Gupta, S., Barrett, T., Whitmarsh, A.J., Cavanagh, J., Sluss, H.K., Derijard, B., Davis, R.J. (1996). Selective interaction of JNK protein kinase isoforms with transcription factors. EMBO J. 15: 2760-2770.
9. Lange-Carter, C.A., Pleiman, C.M., Gardner, A.M., Blumer, K.J., Johnson, G.L. (1993). A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf. Science 260: 315-319.
10. Braunwalder, A.F., Yarwood, D.R., Sills, M.A., Lipson, K.E. (1996). Measurement of the protein tyrosine kinase activity of c-src using time-resolved fluorometry of europium chelates. Anal. Biochem. 238: 159-164.
11. Goldman, M.E., Walton, T.D., Ransone, L.J., Suto, C.M. (1995). An integrated laboratory automation system for quantitative high throughput screening (QHTS) with cell-based assays. ISLAR ‘95 Proceedings 125-137.
12. Smith, S.B., Johnson, K.S. (1988). Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67: 31-40.
13. Vaughn, J.M., Rivier, J., Corrigan, A.Z., McClintock, R., Campen, C.A., Jolley, D., Voglmayer, J.K., Bardin, C.W., Rivier, C., Vale, W. (1989). Detection and purification of inhibin using antisera generated against synthetic peptide fragments. In: Methods in Enzymology, P.M. Conn (ed.) 168:588-617. Academic Press, Orlando, FL.
14. Ginty, D.D., Kornhauser, J.M., Thompson, M.A., Bading, H., Mayo, K.E., Takahashi, J.S., Greenberg, M.E. (1993). Regulation of CREB phosphorylation in the superchiasmatic nuclus by light and circadian clock. Science 260: 238-241.
15. Miranti, C.K., Ginty, D.D., Huang, G., Chatila, T., Greenberg, M.E. (1995). Calcium activates serum response factor-dependent transcription by a ras- and Elk-l-independent mechanism that involves a Ca2+/calmodulin-dependent kinase. Mol. Cell. Biol. 15: 3672-3684.
16. Levitzki, A., Gazit, A. (1995). Tyrosine kinase inhibition: An approach to drug development. Science 267: 1782-1788.
17. Kovalinko, M., Ronnstrand, L., Heldin, C.H., Loubtchenkov, M., Gazit, A., Levitzki, A., Bohmer, F.D. (1997). Phosphorylation site-specific inhibition of platelet-derived growth factor-receptor autophosphorylation by the receptor blocking tyrphostin AG1296. Biochem. 36: 6260-6269.
18. Hemmila, I.A. (1991). Fluorescence energy-transfer immunoassays. In: Application of Fluorescence in Immunoassays. J.D. Wineforder, I.M. Kolthoff (eds.), 117:199-201. Wiley, Inc., New York.
19. Mathis, G. (1995). Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer. Clin. Chem. 41: 1391-1397.