VceR regulates the vceCAB drug efflux pump operon of Vibrio cholerae by alternating between mutually exclusive conformations that bind either drugs or promoter DNA

Journal of Molecular Biology

Volumn 349, Issue 2, 2005, Pages 387-400

  Borges Walmsley, M Ines ^a   Du, Dijun ^a   McKeegan, Kenneth S ^a   Sharples, Gary J ^a   Walmsley, Adrian R ^a   Thomas, J O ^b  

^a DURHAM UNIVERSITY   (United Kingdom)

^b NONE

Author keywords

Antibiotics; Drug resistance; Efflux pump; Repressor; Transporter

Indexed keywords

CARBONYL CYANIDE 4 (TRIFLUOROMETHOXY)PHENYLHYDRAZONE; CARBONYL CYANIDE CHLOROPHENYLHYDRAZONE; CHLORAMPHENICOL; DIMER; DNA; ERYTHROMYCIN; MONOMER; RIFAMPICIN;

ARTICLE; BINDING AFFINITY; CONFORMATION; CONTROLLED STUDY; CORRELATION COEFFICIENT; DISSOCIATION; DNA BINDING; DRUG BINDING; EQUILIBRIUM CONSTANT; FLUORESCENCE SPECTROSCOPY; GEL PERMEATION CHROMATOGRAPHY; GENETIC CODE; GENETIC STRAIN; HILL REACTION; LIGHT SCATTERING; MOLECULAR DYNAMICS; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PURIFICATION; REGULATORY MECHANISM; REPRESSOR GENE; TRANSCRIPTION REGULATION; VIBRIO CHOLERAE;

VIBRIO CHOLERAE;

EID: 18844399841     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2005.03.045     Document Type: Article

References (32)

1. M.I. Borges-Walmsley, and A.R. Walmsley The structure and function of drug pumps Trends Microbiol. 9 2001 71 79
2. M.I. Borges-Walmsley, K.S. McKeegan, and A.R. Walmsley Structure and function of efflux pumps that confer resistance to drugs Biochem. J. 376 2003 313 338
3. K.S. McKeegan, M.I. Borges-Walmsley, and A.R. Walmsley The structure and function of drug pumps: an update Trends Microbiol. 11 2003 21 29
4. +-driven multidrug efflux pump VcmA from Vibrio cholerae non-O1, a non-halophilic bacterium FEMS Microbiol. Letters 203 2001 235 239
5. Y. Morita, A. Kataoka, S. Shiota, T. Mizushima, and T. Tsuchiya NorM of Vibrio parahaemolyticus is an Na(+)-driven multidrug efflux pump J. Bacteriol. 182 2000 6694 6697
6. H.W. van Veen, K. Venema, H. Bolhuis, I. Oussenko, J. Kok, B. Poolman, A.J. Driessen, and W.N. Konings Multidrug resistance mediated by a bacterial homolog of the human multidrug transporter MDR1 Proc. Natl Acad. Sci. USA 93 1996 10668 10672
7. N. Kobayashi, K. Nishino, and A. Yamaguchi Novel macrolide-specific ABC-type efflux transporter in Escherichia coli J. Bacteriol. 183 2001 5639 5644
8. O. Lomovskaya, and K. Lewis emr, an Escherichia coli locus for multidrug resistance Proc. Natl Acad. Sci. USA 89 1992 8938 8942
9. M.I. Borges-Walmsley Identification of oligomerization and drug-binding domains of the membrane fusion protein EmrA J. Biol. Chem. 278 2003 12903 12912
10. J.A. Colmer, J.A. Fralick, and A.N. Hamood Isolation and characterization of a putative multidrug resistance pump from Vibrio cholerae Mol. Microbiol. 27 1998 63 72
11. E.H. Lee, and W.M. Shafer The farAB-encoded efflux pump mediates resistance of gonococci to long-chained antibacterial fatty acids Mol. Microbiol. 33 1999 839 845
12. Federici, L., Du, D., Walas, F., Matsumura, H., Fernandez-Recio, J., McKeegan, K. S. et al. (2005) The crystal structure of the outer membrane protein VceC from the bacterial pathogen Vibrio cholerae at 1.8 Å resolution. J. Biol. Chem. 280, 15307-15314.
13. S. Grkovic, M.H. Brown, and R.A. Skurray Regulation of bacterial drug export systems Microbiol. Mol. Biol. Rev. 66 2002 671 701
14. M.A. Schumacher, and R.G. Brennan Structural mechanisms of multidrug recognition and regulation by bacterial multidrug transcription factors Mol. Microbiol. 45 2002 885 893
15. M.H. Godsey, E.E. Zheleznova Heldwein, and R.G. Brennan Structural biology of bacterial multidrug resistance gene regulators J. Biol. Chem. 277 2002 40169 40172
16. E.E. Zheleznova, P.N. Markham, A.A. Neyfakh, and R.G. Brennan Structural basis of multidrug recognition by BmrR, a transcription activator of a multidrug transporter Cell 96 1999 353 362
17. E.E. Heldwein, and R.G. Brennan Crystal structure of the transcription activator BmrR bound to DNA and a drug Nature 409 2001 378 382
18. M.A. Schumacher, M.C. Miller, S. Grkovic, M.H. Brown, R.A. Skurray, and R.G. Brennan Structural mechanisms of QacR induction and multidrug recognition Science 294 2001 2158 2163
19. M.A. Schumacher, M.C. Miller, S. Grkovic, M.H. Brown, R.A. Skurray, and R.G. Brennan Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR EMBO J. 21 2002 1210 1218
20. S. Grkovic, K.M. Hardie, M.H. Brown, and R.A. Skurray Interactions of the QacR multidrug-binding protein with structurally diverse ligands: implications for the evolution of the binding pocket Biochemistry 42 2003 15226 15236
21. H. Aramaki, N. Yagi, and M. Suzuki Residues important for the function of a multihelical DNA binding domain in the new transcription factor family of Cam and Tet repressors Protein Eng. 8 1995 361 398
22. P. Orth, D. Schnappinger, W. Hillen, W. Saenger, and W. Hinrichs Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system Nature Struct. Biol. 7 2000 215 219
23. S. Grkovic, M.H. Brown, M.A. Schumacher, R.G. Brennan, and R.A. Skurray The Staphylococcal QacR multidrug regulator binds a correctly spaced operator as a pair of dimers J. Bacteriol. 183 2001 7102 7109
24. J.F. Heidelberg, J.A. Eisen, W.C. Nelson, R.A. Clayton, M.L. Gwinn, and R.J. Dodson DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae Nature 406 2000 477 483
25. E.R. Schreiter, M.D. Sintchak, Y. Guo, P.T. Chivers, R.T. Sauer, and C.L. Drennan Crystal structure of the nickel-responsive transcription factor NikR Nature Struct. Biol. 10 2003 794 799
26. M.A. Schumacher, M.C. Miller, and R.G. Brennan Structural mechanism of the simultaneous binding of two drugs to a multidrug-binding protein EMBO J. 23 2004 2923 2930
27. C.C. Hase, L.S. Thai, M. Boesman-Finkelstein, V.L. Mar, W.N. Burnette, and H.R. Kaslow Construction and characterization of recombinant Vibrio cholerae strains producing inactive cholera toxin analogs Infect. Immun. 62 1994 3051 3057
28. B. Miroux, and J.E. Walker Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels J. Mol. Biol. 260 1996 289 298
29. H. Nakamura Novel acriflavine resistance genes, acrC and acrD, in Escherichia coli K-12 J. Bacteriol. 139 1979 8 12
30. NCCLS Document M7-A2, Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically vol. 10, No. 8.
31. D. Ma, D.N. Cook, M. Alberti, N.G. Pon, H. Nikaido, and J.E. Hearst Molecular cloning and characterization of acrA and acrE genes of Escherichia coli J. Bacteriol. 175 1993 6299 6313
32. H. Nikaido, and H.I. Zgurskaya AcrAB and related multidrug efflux pumps of Escherichia coli J. Mol. Microbiol. Biotechnol. 3 2001 215 218