Detection of Enterocytozoon bieneusi (microsporidia) by polymerase chain reaction (PCR) using species-specific primer in stool samples of HIV patients

Indian Journal of Medical Research

Volumn 121, Issue 4, 2005, Pages 215-219

  Satheesh Kumar, S ^a   Ananthan, Sowanand ^a   Joyee, A G ^a  

^a UNIVERSITY OF MADRAS   (India)

Author keywords

Diarrhoea; Enterocytozoon bieneusi; HIV; Microsporidia; PCR

Indexed keywords

DNA FRAGMENT; SMALL SUBUNIT RIBOSOMAL RNA;

ARTICLE; CHRONIC DIARRHEA; CLINICAL OBSERVATION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; ENTEROCYTOZOON; FECES ANALYSIS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; MAJOR CLINICAL STUDY; MICROSCOPY; MICROSPORIDA; NUCLEIC ACID AMPLIFICATION; NUCLEOTIDE SEQUENCE; PROTOZOAN SPORE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPECIES DIFFERENCE; SPECIES IDENTIFICATION; STAINING;

BASE SEQUENCE; DIARRHEA; DNA PRIMERS; ENTEROCYTOZOON; FECES; HIV INFECTIONS; HUMANS; POLYMERASE CHAIN REACTION; RNA, PROTOZOAN;

EID: 18844383163     PISSN: 09715916     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Weber R, Bryan RT, Schwartz DA, Owen RL. Human microsporidia infections. Clin Microbiol Rev 1994; 7 : 426-61.
2. Muller A, Stellermann K, Hartmann P, Schrappe M, Fatkenheuer G, Salzberger B, et al. A powerful DNA extraction method and PCR for detection of microsporidia in clinical stool specimens. Clin Diag Lab Immunol 1999; 6 : 243-6.
3. Da silva AJ, Bornay-Llinares FJ, De la Puente CA, Moura H, Peralta JM, Sobottka I, et al. Diagnosis of Enterocytozoon bieneusi (Microsporidia) infections by polymerase chain reaction in stool samples using primers based on the region coding for small-subunit ribosomal RNA. Arch Pathol Lab Med 1997; 121 : 874-9.
4. Modigliani R, Bories C, Le Charpentier Y, Salmeron M, Messing B, Galian A, et al. Diarrhoea and malabsorption in acquired immume deficiency syndrome : a study of four cases with special emphasis on opportunistic protozoan infestations. Gut 1985; 26 : 179-87.
5. Coyle CM, Wittner M, Kotler DP, Noyer C, Orenstein JM, Tanowitz HB, et al. Prevalence of microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon (Septata) intestinalis among patients with AIDS-related diarrhoea: Determination by polymerase chain reaction to the microsporidian small-subunit rRNA gene. Clin Infect Dis 1996; 23 : 1002-6.
6. Weber R, Bryan RT, Owen RL, Wilcox CM, Gorelkin L, Visvesvara GS. Improved light-microscopical detection of microsporidia spores in stool and duodenal aspirates. N Engl J Med 1992; 326 : 161-6.
7. Da silva AJ, Slemenda SB, Visvesvara GS, Schwartz DA, Mel Wilcox C, Wallace S, et al. Detection of Septata intestinalis (Microsporidia) Cali et al. 1993 Using polymerase chain reaction primers targeting the small subunit ribosomal RNA coding region. Mol Diagn 1997; 2 : 47-52.
8. Velasquez JN, Carnevale S, Guarnera EA, Labbe JH, Chertcoff A, Cabrera MG, et al. Detection of the microsporidian parasite Enterocytozoon bieneusi in specimens from patients with AIDS by PCR. J Clin Microbiol 1996; 34 : 3230-2.
9. Ash LR, Orihel TC. Collection and preservation of faeces. In : Parasites : A guide to laboratory procedures and identification - Ist Edtion, Chicago: American Society of Clincal Pathologist Press; 1987 p. 5-53.
10. Van Gool T, Canning EU, Dankert J. An improved practical and sensitive technique for the detection of microsporidian in stool samples. Trans R Soc Trop Med Hyg 1994; 88 : 189-90.
11. Ma P, Soave R. Three-step stool examination for cryptosporidiosis in 10 homosexual men with protracted watery diarrhoea. J Infect Dis 1983; 147 : 824-8.
12. Da silva AJ, Schwartz DA, Visvesvara GS, Moura H, Slemenda SB, Pieniazek NJ. Sensitive PCR diagnosis of infections by Enterocytozoon bieneusi (Microsporidia) using primers based on the region coding for small-subunit rRNA. J Clin Microbiol 1996 ; 34 : 986-7.
13. Hartskeerl RA, Schuitema ARJ, Van Goof T, Terpstra WJ. Genetic evidence for the occurrence of extra-intestinal Enterocytozoon bieneusi infections. Nucleic Acid Res 1993; 21 : 4150.
14. Franzen C, Muller A. Molecular techniques for detection, species differentiation, and a phylogenetic analysis of microsporidia. Clin Microbiol Rev 1999; 12 : 243-85.
15. Katzwinkel-Wladarsch S, Deplazes P, Weber R, Loscher T, Rinder H. Comparison of polymerase chain reaction with light microscopy for detection of microsporidia in clinical specimens. Eur J Clin Microbiol Infect Dis 1997; 16 : 7-10.
16. Ombrouck C, Ciceron L, Biligui S, Brown S, Marechal P, Van Gool T, et al. Specific PCR assay for direct detection of intestinal microsporidia Enterocytozoon bieneusi and Encephalitozoon intestinalis in fecal specimens from human immunodeficiency virus-infected patients. J Clin Microbiol 1997; 35 : 652-5.
17. Blanshard C, Ellis Tovey DG, Dowell S, Gazzard BG. Treatment of intestinal microsporidiosis with albendazole in patients with AIDS. AIDS 1992; 6 : 311-3.