Effect of dimethyl sulphoxide on the crystal structure of porcine pepsin

Biochemical and Biophysical Research Communications

Volumn 331, Issue 4, 2005, Pages 1510-1514

  Kesavulu, Muppuru Muni ^a   Ramasubramanian, Sundaramoorthy ^a   Suguna, Kaza ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

Dimethyl sulphoxide; Pepsin; Protein denaturation

Indexed keywords

DIMETHYL SULFOXIDE; PEPSIN A; WATER;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CIRCULAR DICHROISM; CRYSTAL STRUCTURE; CRYSTALLIZATION; DENSITY; ELECTRON; ENZYME ASSAY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN STABILITY; PROTEIN STRUCTURE; SWINE; TEMPERATURE DEPENDENCE; X RAY CRYSTALLOGRAPHY;

ANIMALS; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; DIMETHYL SULFOXIDE; MODELS, MOLECULAR; PEPSIN A; SWINE;

SUIDAE;

EID: 18844373053     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.03.247     Document Type: Article

References (16)

1. J. Berthou, and P. Jolles The influence of urea on crystallization and polymorphism of hen lysozyme FEBS Lett. 31 1973 189 192
2. K.W. Snape, R. Tjian, C.C.F. Blake, and D.E. Koshland Crystallographic study of the interaction of urea with lysozyme Nature 250 1974 295 298
3. A.C.W. Pike, and K.R. Acharya A structural basis for the interaction of urea with lysozyme Protein Sci. 3 1994 706 710
4. S.C. Mande, and M.E. Sobhia Structural characterization of protein-denaturant interactions: crystal structures of hen egg-white lysozyme in complex with DMSO and guanidinium chloride Protein Eng. 13 2000 133 141
5. L.S. Hibbard, and A. Tulinsky Expression of functionality of α-chymotrypsin. Effects of guanidine hydrochloride and urea in the onset of denaturation Biochemistry 17 1978 5460 5468
6. J. Dunbar, H.P. Yennawar, S. Banerjee, J. Luo, and G.K. Farber The effect of denaturants on protein structure Protein Sci. 6 1997 1727 1733
7. G.S. Ratnaparkhi, and R. Varadarajan X-ray crystallographic studies of the denaturation of ribonuclease S Proteins: Struct. Funct. Genet. 36 1999 282 294
8. J.B. Cooper, G. Khan, G. Taylor, I.J. Tickle, and T.L. Blundell X-ray analysis of aspartic proteinases. II. Three-dimensional structure of the hexagonal crystal form of porcine pepsin at 2.3 Å resolution J. Mol. Biol. 214 1990 199 222
9. Z. Otwinowski, and W. Minor Processing of X-ray diffraction data collected in oscillation mode Methods Enzymol. 276 1997 307 326
10. A.T. Brunger, P.D. Adams, G.M. Clore, W.L. DeLano, P. Gros, R.W. Grosse-Kunstleve, J.S. Jiang, J. Kuszewski, M. Nilges, N.S. Pannu, R.J. Read, L.M. Rice, T. Simonson, and G.L. Warren Crystallography and NMR system: a new software suite for macro molecular structure determination Acta Crystallogr. D 54 1998 905 921
11. T.A. Jones, J.Y. Zou, S.W. Cowan, and M. Kjeldgaard Improved methods for building protein models in electron density maps and the location of errors in these models Acta Crystallogr. A 47 1991 110 119
12. Available from: 〈http://www.worthington-biochem.com/PM/default. html〉
13. M. Anson The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin J. Gen. Physiol. 22 1938 79 89
14. G.E. Cohen ALIGN: a program to superimpose protein coordinates, accounting for insertions and deletions J. Appl. Crystallogr. 30 1997 1160 1161
15. R.M. Esnouf An extensively modified version of MolScript that includes greatly enhanced coloring capabilities J. Mol. Graph. 15 1997 132 134
16. E.A. Merritt, and D.J. Bacon Raster3D: photorealistic molecular graphics Methods Enzymol. 277 1997 505 524