The mutagenic effect of ultraviolet-A1 on human skin demonstrated by sequencing the p53 gene in single keratinocytes

Photodermatology Photoimmunology and Photomedicine

Volumn 18, Issue 6, 2002, Pages 287-293

  Persson, Åsa E ^a   Edström, Desiree W ^b   Bäckvall, Helena ^c   Lundeberg, Joakim ^a   Pontén, Fredrik ^c   Ros, Anne Marie ^b   Williams, Cecilia ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^c UPPSALA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Mutations; P53; Single cells; UV A1

Indexed keywords

PROTEIN P53;

ARTICLE; BUTTOCK; CELL CYCLE PHASE; CONTROLLED STUDY; EPIDERMIS; EXON; FEMALE; GENE MUTATION; GENE SEQUENCE; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; HUMAN TISSUE; IMMUNOCOMPETENT CELL; IMMUNOREACTIVITY; INTRON; KERATINOCYTE; MUTAGENIC ACTIVITY; NORMAL HUMAN; PRIORITY JOURNAL; PUNCH BIOPSY; RADIATION DOSE; RADIATION EXPOSURE; SKIN CANCER; TUMOR SUPPRESSOR GENE; ULTRAVIOLET A RADIATION;

DNA PRIMERS; GENES, P53; HUMANS; KERATINOCYTES; MUTATION; POLYMERASE CHAIN REACTION; ULTRAVIOLET RAYS;

EID: 18744405673     PISSN: 09054383     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0781.2002.02781.x     Document Type: Article

References (28)

1. Fitzpatrick TB. Ultraviolet-induced pigmentary changes: benefits and hazards. Curr Probl Dermatol 1986; 15: 25-38.
2. Diffey BJ. Phtodermatology, Arnold, London 1999.
3. Nishigori C, Yarosh DB, Donawho C, Kripke ML. The immune system in ultraviolet carcinogenesis. J Invest Dermatol Symp Proc 1996; 1: 143-146.
4. Tornaletti S, Pfeifer GP. UV damage and repair mechanisms in mammalian cells. Bioessays 1996; 18: 221-228.
5. Young AR, Potten CS, Nikaido O, et al. Human melanocytes and keratinocytes exposed to UVB or UVA in vivo show comparable levels of thymine dimers. J Invest Dermatol 1998; 111: 936-940.
6. Cadet J, Berger M, Douki T, et al. Effects of UV and visible radiation on DNA-final base damage. Biol Chem 1997; 378: 1275-1286.
7. Kielbassa C, Epe B. DNA damage induced by ultraviolet and visible light and its wavelength dependence. Meth Enzymol 2000; 319: 436-445.
8. Kawanishi S, Hiraku Y, Oikawa S. Mechanism of guanine-specific DNA damage by oxidative stress and its role in carcinogenesis and aging. Mutat Res 2001; 488: 65-76.
9. Runger TM. Role of UVA in the pathogenesis of melanoma and non-melanoma skin cancer: a short review. Photodermatol Photoimmunol Photomed 1999; 15: 212-216.
10. Epe B. Genotoxicity of singlet oxygen. Chem Biol Interact 1991; 80: 239-260.
11. Cheng KC, Cahill DS, Kasai H, Nishimura S, Loeb LA. 8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G-T and A-C substitutions. J Biol Chem 1992; 267: 166-172.
12. Drobetsky EA, Turcotte J, Chateauneuf A. A role for ultraviolet A in solar mutagenesis. Proc Natl Acad Sci USA 1995; 92: 2350-2354.
13. Kvam E, Tyrrell RM. Induction of oxidative DNA base damage in human skin cells by UV and near visible radiation. Carcinogenesis 1997; 18: 2379-2384.
14. Pontén F, Lindman H, Bostrom A, Berne B, Bergh J. Induction of p53 expression in skin by radiotherapy and UV radiation: a randomized study. J Natl Cancer Inst 2001; 93: 128-133.
15. Pontén F, Berne B, Ren ZP, Nistér M, Pontén J. Ultraviolet light induces expression of p53 and p21 in human skin: effect of sunscreen and constitutive p21 expression in skin appendages. J Invest Dermatol 1995; 105: 402-406.
16. Burren R, Scaletta C, Frenk E, Panizzon RG, Applegate LA. Sunlight and carcinogenesis: expression of p53 and pyrimidine dimers in human skin following UVA I, UVA I + II and solar simulating radiations. Int J Cancer 1998; 76: 201-206.
17. Edstrom DW, Porwit A, Ros AM. Effects on human skin of repetitive ultraviolet-A1 (UV-A1) irradiation and visible light. Photodermatol Photoimmunol Photomed 2001; 17: 66-70.
18. Seite S, Moyal D, Verdier MP, Hourseau C, Fourtanier A. Accumulated p53 protein and UVA protection level of sunscreens. Photodermatol Photoimmunol Photomed 2000; 16: 3-9.
19. Schutze K, Lahr G. Identification of expressed genes by laser-mediated manipulation of single cells. Nat Biotechnol 1998; 16: 737-742.
20. Berg C, Hedrum A, Holmberg A, Pontén F, Uhlén M, Lundeberg J. Direct solid-phase sequence analysis of the human p53 gene by use of multiplex polymerase chain reaction and alpha-thiotriphosphate nucleotides. Clin Chem 1995; 41: 1461-1466.
21. Pontén F, Williams C, Ling G, et al. Genomic analysis of single cells from human basal cell cancer using laser-assisted capture microscopy. Mut Res 1997; 382: 45-55.
22. Persson AE, Ling G, Williams C, et al. Analysis of p53 mutations in single cells obtained from histological tissue sections. Anal Biochem 2000; 287: 25-31.
23. Ren ZP, Hedrum A, Pontén F, et al. Human epidermial cancer and accompanying precursors have identical p53 mutations different from p53 mutations in adcent areas of clonally expanded non-neoplastic keratinocytes. Oncogene 1996; 12: 765-773.
24. Schutze K, Clement-Sengewald A. Catch and move-cut fuse. Nature 1994; 368: 667-669.
25. Ling G, Persson A, Berne B, Uhlen M, Lundeberg J, Ponten F. Persistent p53 mutations in single cells from normal human skin. Am J Pathol 2001; 159: 1247-1253.
26. Arbabi L, Gange RW, Parrish JA. Recovery of skin from a single suberythemal dose of ultraviolet radiation. J Invest Dermatol 1983; 81: 78-82.
27. Andre P, Kim A, Khrapko K, Thilly WG. Fidelity and mutational spectrum of Pfu DNA polymerase on a human mitochondrial DNA sequence. Genome Res 1997; 7: 843-852.
28. Oller AR, Rastogi P, Morgenthaler S, Thilly WG. A statistical model to estimate variance in long-term low-dose mutation assays: testing of the model in a human lymphoblastoid mutation assay. Mutat Res 1989; 216: 149-161.