Analysis of p53 mutations in single cells obtained from histological tissue sections

Analytical Biochemistry

Volumn 287, Issue 1, 2000, Pages 25-31

  Persson, Åsa E ^a   Ling, Gao ^b   Williams, Cecilia ^b   Bäckvall, Helena ^b   Pontén, Jan ^b   Pontén, Fredrik ^b   Lundeberg, Joakim ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b UPPSALA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLOGY; GENES; TISSUE;

AMPLIFICATION EFFICIENCIES; DIRECT SEQUENCE; GENE AMPLIFICATION; GENETIC ANALYSIS; HIGH FREQUENCY HF; HISTOLOGICAL TISSUE; SAMPLE PREPARATION; TISSUE SECTIONS;

TISSUE ENGINEERING;

PROTEIN P53;

ARTICLE; CARCINOGENESIS; DNA DEGRADATION; GENE AMPLIFICATION; GENE MUTATION; GENE SEQUENCE; GENETIC ANALYSIS; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SEQUENCE ANALYSIS; TUMOR SUPPRESSOR GENE;

EID: 0034544770     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4830     Document Type: Article

References (25)

1. Laser capture microdissection
2. Laser capture microdissection of single cells from complex tissues
3. Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer
4. PCR amplification of megabase DNA with tagged random primers (T-PCR)
5. Whole genome amplification from a single cell: Implications for genetic analysis
6. Detailed chromosomal and molecular genetic analysis of single cells by whole genome amplification and comparative genomic hybridisation
7. Genomic analysis of single cells from human basal cell cancer using laser-assisted capture microscopy
8. Molecular pathology in basal cell cancer with p53 as a genetic marker
9. Sequence-based analysis of the human p53 gene based on microdissection of tumor biopsy samples
10. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line
11. Catch and move - Cut or fuse: Review
12. Direct solid-phase sequence analysis of the human p53 gene by use of multiplex polymerase chain reaction and alpha-thiotriphosphate nucleotides
13. Fidelity and mutational spectrum of Pfu DNA polymerase on a human mitochondrial DNA sequence
14. Highly accurate analysis of heterozygous loci by single cell PCR
15. Multiple genetic diagnoses from single cells using multiplex PCR: Reliability and allele dropout
16. Allele drop-out in polar bodies and blastomeres
17. Efficiency of single-cell polymerase chain reaction from stained histologic slides and integrity of DNA in archival tissue
18. Molecular studies on single cells harvested by micromanipulation from archival tissue sections previously stained by immunohistochemistry or nonisotopic in situ hybridization
19. High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus
20. Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase
21. DNA polymerase fidelity and the polymerase chain reaction
22. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support
23. DNA damage promotes jumping between templates during enzymatic amplification
24. A high frequency of sequence alterations is due to formalin fixation of archival specimens
25. Context-dependent taq-polymerase-mediated nucleotide alterations, as revealed by direct sequencing of the ZNF189 gene: Implications for mutation detection